Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging
Glycolysis is the metabolic pathway that converts glucose into pyruvate, whereas fermentation can then produce lactate from pyruvate. Here, we developed single fluorescent protein (FP)-based lactate and pyruvate indicators with low EC50 for hint detection of metabolic molecules and live cell imaging and named them “Green Lindoblum” and “Green Pegassos,” respectively.
Green Lindoblum (EC50 of 30 µM for lactate) and Green Pegassos (EC50 of 70 µM for pyruvate) produced a 5.2- and 3.3-fold change in fluorescence depth in response to lactate and pyruvate, respectively. Green Lindoblum measured lactate ranges in mouse plasma, and Green Pegassos together with D-serine dehydratase efficiently estimated D-serine ranges launched from mouse main cultured neurons and astrocytes by measuring pyruvate degree.
Furthermore, live cell imaging evaluation revealed their utility for dual-colour imaging, and the interaction between lactate, pyruvate, and Ca2+ in human induced pluripotent stem cell-derived cardiomyocytes. Therefore, Green Lindoblum and Green Pegassos might be helpful instruments that detect particular molecules in scientific use and monitor the interaction of metabolites and different associated molecules in various cell varieties.
Promoter Pull-Down Assay: A Biochemical Screen for DNA-Binding Proteins
Transcription components are ubiquitous proteins that affiliate with promoter DNA and regulate gene expression by way of quite a lot of mechanisms. Understanding transcriptional management mechanisms requires in-depth investigation of the binding of transcription components to the promoters they regulate.
There are many in vivo and in vitro strategies for testing the binding of a identified protein to a promoter, reminiscent of chromatin immunoprecipitation and electrophoretic mobility shift assays. However, for these experiments, one will need to have a protein candidate to check and is just not capable of determine unknown proteins sure to a selected promoter.
Thus, the promoter pull-down assay was developed to fill this void. This technique makes use of DNA as bait to seize proteins that bind to a particular promoter, reminiscent of transcription components, from mobile lysates. Coupled with different experiments, the promoter pull-down assay vastly improves the repertoire of strategies obtainable for defining regulatory complexes that affect transcription.
Target-Based Identification and Optimization of 5-Indazol-5-yl Pyridones as Toll-like Receptor 7 and 8 Antagonists Using a Biochemical TLR8 Antagonist Competition Assay
Inappropriate activation of endosomal TLR7 and TLR8 happens in a number of autoimmune illnesses, particularly systemic lupus erythematosus (SLE). Herein, the event of a TLR8 antagonist competitors assay and its software for hit era of twin TLR7/Eight antagonists are reported.
The structure-guided optimization of the pyridone hit <b>3</b> utilizing this biochemical assay together with mobile and TLR8 cocrystal structural information resulted within the identification of a extremely potent and selective TLR7/Eight antagonist (<b>27</b>) with <i>in vivo</i> efficacy.
The two key steps for optimization have been (i) a core morph guided by a TLR7 sequence alignment to attain a twin TLR7/Eight antagonism profile and (ii) introduction of a fluorine within the piperidine ring to scale back its basicity, leading to enticing oral pharmacokinetic (PK) properties and improved TLR8 binding affinity.
Hunting monolignol transporters: membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce
Monolignol transport throughout lignification is {a partially} solved puzzle: each the mechanism(s) and the transported type of monolignols are unknown in creating xylem of timber. We examined a speculation of an energetic, plasma membrane (PM)-localized transport of monolignol monomers, dimers, and/or glucosidic types with membrane vesicles ready of creating xylem and lignin-forming tissue-cultured cells of Norway spruce (Picea abies L. Karst.), in addition to of management supplies, non-lignifying Norway spruce phloem and tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported each coniferin and p-coumaryl alcohol glucoside, however inhibitor assays prompt this transport being over the tonoplast.
Also membrane vesicles ready from the lignin-forming spruce cells confirmed coniferin transport, however the Km for coniferin was a lot increased than these of xylem and BY-2 cells. Liquid chromatography-mass spectrometry (LC-MS/MS) evaluation of membrane proteins remoted from spruce creating xylem, phloem and lignin-forming cultured cells revealed a number of transporters.
These have been in comparison with a transporter gene set that was gained by a correlation evaluation with a particular set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays confirmed no help for the ABC-transporter-mediated monolignol transport however level to secondary energetic transporters (reminiscent of MFS or MATE transporters). In distinction, proteomic and co-expression analyses counsel a job for ABC transporters and MFS transporters.
Protective Mechanism of Acacia saligna Butanol Extract and Its Nano-Formulations in opposition to Ulcerative Colitis in Rats as Revealed by way of Biochemical and Metabolomic Assays
Ulcerative colitis (UC) is a relapsing inflammatory illness of unknown etiology. The elevated danger of most cancers in UC sufferers warrants for the event of novel drug remedies. Herein, this work issues with the investigation of the protecting results of Acacia saligna butanol extract (ASBE) and its nanoformulations on UC in a rat mannequin and its underlying mechanism. Colitis was induced by gradual intrarectal infusion of two mL of 4% (v/v in 0.9% saline) acetic acid. Colon samples have been evaluated macroscopically, microscopically, and assayed for pro-inflammatory cytokine ranges.
To monitor related metabolic adjustments in acetic acid-induced UC mannequin, serum samples have been analyzed for main metabolites utilizing GC-MS adopted by multivariate information analyses. Treatment with ASBE attenuated acetic acid-induced UC as revealed by discount of colon weight, ulcer space, and ulcer index.
ASBE remedy additionally decreased Cyclooxygenase-2 (COX-2), Prostaglandin E2 (PGE2) & Interleukin-1β (IL-1β) ranges within the infected colon. The nano-formulation of ASBE confirmed higher safety than the crude extract in opposition to ulcer indices, elevated PGE2 manufacturing, and histopathological alterations reminiscent of intestinal mucosal lesions and inflammatory infiltration.
Distinct metabolite adjustments have been recorded in colitis rats together with a lower in oleamide and arachidonic acid together with elevated ranges of lactic acid, fructose, and pyroglutamic acid.
 ELISA Kit) Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| DLR-bACE1-Mu-48T | DL Develop | 48T | 586.8 EUR |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) in samples from serum, plasma, tissue homogenates, cerebrospinal fluid or other biological fluids. |
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Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| DLR-bACE1-Mu-96T | DL Develop | 96T | 762 EUR |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) in samples from serum, plasma, tissue homogenates, cerebrospinal fluid or other biological fluids. |
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 ELISA Kit) Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Hu-48Tests | Reddot Biotech | 48 Tests | 600 EUR |
Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Hu-96Tests | Reddot Biotech | 96 Tests | 830.4 EUR |
Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Mu-48Tests | Reddot Biotech | 48 Tests | 613.2 EUR |
Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Mu-96Tests | Reddot Biotech | 96 Tests | 850.8 EUR |
 ELISA Kit) Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Ra-48Tests | Reddot Biotech | 48 Tests | 640.8 EUR |
Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Ra-96Tests | Reddot Biotech | 96 Tests | 890.4 EUR |
Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RD-bACE1-Hu-48Tests | Reddot Biotech | 48 Tests | 573.6 EUR |
Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RD-bACE1-Hu-96Tests | Reddot Biotech | 96 Tests | 794.4 EUR |
Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RD-bACE1-Mu-48Tests | Reddot Biotech | 48 Tests | 586.8 EUR |
Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RD-bACE1-Mu-96Tests | Reddot Biotech | 96 Tests | 812.4 EUR |
Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RD-bACE1-Ra-48Tests | Reddot Biotech | 48 Tests | 613.2 EUR |
Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RD-bACE1-Ra-96Tests | Reddot Biotech | 96 Tests | 850.8 EUR |
 Bace1/ Rat Bace1 ELISA Kit |
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| ELI-02505r | Lifescience Market | 96 Tests | 1063.2 EUR |
 Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed |
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| ELISA-1 | Alpha Diagnostics | 1 | 242.4 EUR |
 BACE1 |
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| RA21010 | Neuromics | 50 ug | 522 EUR |
 Mouse bACE1 ELISA Kit |
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| EMB0322 | Abclonal | 96Tests | 625.2 EUR |
 Sheep BACE1 ELISA Kit |
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| ESB0322 | Abclonal | 96Tests | 625.2 EUR |
 Human BACE1 ELISA Kit |
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| EHB0322 | Abclonal | 96Tests | 625.2 EUR |
 Rat BACE1 ELISA Kit |
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| ERB0322 | Abclonal | 96Tests | 625.2 EUR |
 Monkey BACE1 ELISA Kit |
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| EMKB0322 | Abclonal | 96Tests | 625.2 EUR |
 Porcine BACE1 ELISA Kit |
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| EPB0322 | Abclonal | 96Tests | 625.2 EUR |
 Rabbit BACE1 ELISA Kit |
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| ERTB0322 | Abclonal | 96Tests | 625.2 EUR |
 Goat BACE1 ELISA Kit |
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| EGTB0322 | Abclonal | 96Tests | 625.2 EUR |
 Bovine BACE1 ELISA Kit |
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| EBB0322 | Abclonal | 96Tests | 625.2 EUR |
 Canine BACE1 ELISA Kit |
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| ECB0322 | Abclonal | 96Tests | 625.2 EUR |
 Chicken BACE1 ELISA Kit |
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| ECKB0322 | Abclonal | 96Tests | 625.2 EUR |
Human BACE1 ELISA Kit |
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| ELA-E0718h | Lifescience Market | 96 Tests | 988.8 EUR |
 BACE1 ELISA KIT|Human |
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| EF006629 | Lifescience Market | 96 Tests | 826.8 EUR |
 Anserini bACE1 ELISA Kit |
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| EAB0322 | Abclonal | 96Tests | 625.2 EUR |
 BACE1 Antibody |
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| 1-CSB-PA173556 | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:15-1:50 |
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BACE1 Antibody |
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| 1-CSB-PA002524GA01HU | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
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BACE1 Antibody |
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| 1-CSB-PA002524LA01HU | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
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BACE1 Antibody |
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| 1-CSB-PA150463 | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000 |
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BACE1 Antibody |
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| 1-CSB-PA008418 | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000 |
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BACE1 Antibody |
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| DF7939 | Affbiotech | 200ul | 420 EUR |
BACE1 Antibody |
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| AF6650 | Affbiotech | 100ul | 420 EUR |
 bACE1 siRNA |
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| 20-abx908826 | Abbexa |
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bACE1 siRNA |
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| 20-abx908827 | Abbexa |
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BACE1 Antibody |
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| 6651-100 | Biovision | 418.8 EUR | |
BACE1 Antibody |
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| 6651-30T | Biovision | 175.2 EUR | |
 BACE1 antibody |
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| 70R-7276 | Fitzgerald | 50 ug | 560.4 EUR |
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Description: Rabbit polyclonal BACE1 antibody raised against the N terminal of BACE1 |
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 Bace1 antibody |
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| 70R-8662 | Fitzgerald | 50 ug | 560.4 EUR |
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Description: Affinity purified rabbit polyclonal Bace1 antibody |
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BACE1 antibody |
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| 70R-15953 | Fitzgerald | 50 ul | 522 EUR |
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Description: Rabbit polyclonal BACE1 antibody |
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BACE1 antibody |
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| 20R-BR014 | Fitzgerald | 50 ug | 787.2 EUR |
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Description: Rabbit polyclonal BACE1 antibody |
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BACE1 antibody |
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| 20R-BR015 | Fitzgerald | 50 ug | 787.2 EUR |
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Description: Rabbit polyclonal BACE1 antibody |
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BACE1 Antibody |
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| 35650-100ul | SAB | 100ul | 302.4 EUR |
BACE1 Antibody |
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| ABD7939 | Lifescience Market | 100 ug | 525.6 EUR |
BACE1 Antibody |
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| ABD7999 | Lifescience Market | 100 ug | 525.6 EUR |
bACE1 siRNA |
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| 20-abx900582 | Abbexa |
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 BACE1 protein |
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| E62B011 | EnoGene | 20ug | 411.6 EUR |
 anti-BACE1 |
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| YF-PA17994 | Abfrontier | 100 ug | 483.6 EUR |
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Description: Rabbit polyclonal to BACE1 |
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BACE1 Antibody |
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| F50757-0.08ML | NSJ Bioreagents | 0.08 ml | 165 EUR |
BACE1 Antibody |
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| F50758-0.08ML | NSJ Bioreagents | 0.08 ml | 165 EUR |
BACE1 Antibody |
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| F50758-0.4ML | NSJ Bioreagents | 0.4 ml | 379 EUR |
 (OKAN05558)) BACE1 ELISA Kit (Human) (OKAN05558) |
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| OKAN05558 | Aviva Systems Biology | 96 Wells | 950.4 EUR |
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Description: Description of target: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.11 ng/mL |
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 (OKAN05559)) BACE1 ELISA Kit (Mouse) (OKAN05559) |
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| OKAN05559 | Aviva Systems Biology | 96 Wells | 950.4 EUR |
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Description: Description of target: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. Homozygous knockout mice for this gene exhibit a wide range of nervous system defects, growth retardation, metabolic abnormalities, and increased neonatal lethality.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 14.1 pg/mL |
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 (OKCD06723)) BACE1 ELISA Kit (Human) (OKCD06723) |
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| OKCD06723 | Aviva Systems Biology | 96 Wells | 903.6 EUR |
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Description: Description of target: Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein. BACE1, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi.Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Four transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.11ng/mL |
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 (OKCD06724)) BACE1 ELISA Kit (Mouse) (OKCD06724) |
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| OKCD06724 | Aviva Systems Biology | 96 Wells | 934.8 EUR |
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Description: Description of target: Bace1 is responsible for the proteolytic processing of the amyloid precursor protein (APP). Bace1 cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 12.1pg/mL |
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 (OKEH03206)) BACE1 ELISA Kit (Mouse) (OKEH03206) |
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| OKEH03206 | Aviva Systems Biology | 96 Wells | 794.4 EUR |
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Description: Description of target: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.39 ng/mL |
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 (OKCD02313)) BACE1 ELISA Kit (Rat) (OKCD02313) |
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| OKCD02313 | Aviva Systems Biology | 96 Wells | 981.6 EUR |
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Description: Description of target: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. ;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 6.7 pg/mL |
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 Guinea Pig BACE1 ELISA Kit |
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| EGB0322 | Abclonal | 96Tests | 625.2 EUR |
 BACE1 Blocking Peptide |
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| DF7939-BP | Affbiotech | 1mg | 234 EUR |
 BACE1 Conjugated Antibody |
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| C35650 | SAB | 100ul | 476.4 EUR |
 BACE1 cloning plasmid |
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| CSB-CL002524HU-10ug | Cusabio | 10ug | 637.2 EUR |
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Description: A cloning plasmid for the BACE1 gene. |
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 anti- BACE1 antibody |
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| FNab00781 | FN Test | 100µg | 658.5 EUR |
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Description: Antibody raised against BACE1 |
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BACE1 Blocking Peptide |
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| 33R-3496 | Fitzgerald | 100 ug | 216 EUR |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of BACE1 antibody, catalog no. 70R-7276 |
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 Antibody) bACE1 (pS498) Antibody |
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| abx148520-100ug | Abbexa | 100 ug | 526.8 EUR |
 BACE1 Polyclonal Antibody |
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| ABP-PAB-31005 | Allele Biotech | 50 ug | Ask for price |
BACE1 Polyclonal Antibody |
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| ABP-PAB-31006 | Allele Biotech | 50 ug | Ask for price |
 BACE1 Rabbit pAb |
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| A5266-100ul | Abclonal | 100 ul | 369.6 EUR |
BACE1 Rabbit pAb |
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| A5266-200ul | Abclonal | 200 ul | 550.8 EUR |
BACE1 Rabbit pAb |
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| A5266-20ul | Abclonal | 20 ul | 219.6 EUR |
BACE1 Rabbit pAb |
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| A5266-50ul | Abclonal | 50 ul | 267.6 EUR |
 BACE1 Recombinant Protein |
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| 96-050 | ProSci | 0.1 mg | 619.8 EUR |
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Description: Beta-secretase 1 (BACE1) is also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. |
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BACE1 Recombinant Protein |
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| 96-051 | ProSci | 0.1 mg | 556.8 EUR |
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Description: Beta-secretase 1 (BACE1) is also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. |
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BACE1 Recombinant Protein |
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| 96-052 | ProSci | 0.2 mg | 556.8 EUR |
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Description: Beta-secretase 1 (BACE1) is also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. |
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 Polyclonal BACE1 Antibody |
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| APG03188G | Leading Biology | 0.1mg | 580.8 EUR |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BACE1 . This antibody is tested and proven to work in the following applications: |
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 Recombinant Human BACE1 |
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| P0562 | FN Test | 100ug | 626.83 EUR |
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Description: Recombinant Human protein for BACE1 |
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 BACE1-IN-1 |
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| HY-100182 | MedChemExpress | 5mg | 2958 EUR |
 Anti-BACE1 antibody |
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| PAab00781 | Lifescience Market | 100 ug | 463.2 EUR |
Anti-BACE1 antibody |
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| STJ29911 | St John's Laboratory | 100 µl | 332.4 EUR |
|
Description: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. |
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 Anti-BACE1 Antibody |
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| STJ193186 | St John's Laboratory | 200 µl | 236.4 EUR |
 Basic Cytotoxicity Test Kits |
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| CT17001 | Neuromics | 125 Tests | 490.8 EUR |
Basic Cytotoxicity Test Kits |
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| CT17002 | Neuromics | 250 Tests | 780 EUR |
 Total Cytotoxicity Test Kits |
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| CT17003 | Neuromics | 125 Tests | 615.6 EUR |
Total Cytotoxicity Test Kits |
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| CT17004 | Neuromics | 250 Tests | 975.6 EUR |
 Necrosis vs Apoptosis Kits |
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| KF17371 | Neuromics | 50-100 Tests | 522 EUR |
Necrosis vs Apoptosis Kits |
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| KF17372 | Neuromics | 100-200 Tests | 921.6 EUR |
 Pipette Stand Starter Kits |
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| P7700-S1 | Benchmark Scientific | 1 PC | 609.4 EUR |
Pipette Stand Starter Kits |
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| P7700-S2 | Benchmark Scientific | 1 PC | 631.32 EUR |
 T&A Cloning Kits |
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| FYC001-20P | Yeastern Biotech | 1 vial | Ask for price |
 Sealed Vials Calibration Kits |
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| 2659505 | Scientific Laboratory Supplies | EACH | 814.6 EUR |
 Carcass Sponge Sampling Kits |
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| SWA1038 | Scientific Laboratory Supplies | PK95 | 332.88 EUR |
 BACE1 Antibody, HRP conjugated |
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| 1-CSB-PA002524LB01HU | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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 BACE1 Antibody, FITC conjugated |
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| 1-CSB-PA002524LC01HU | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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 BACE1 Antibody, Biotin conjugated |
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| 1-CSB-PA002524LD01HU | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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 Rat BACE1 shRNA Plasmid |
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| 20-abx985469 | Abbexa |
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 Antibody) Phospho-BACE1 (Thr252) Antibody |
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| AF3650 | Affbiotech | 100ul | 540 EUR |
 Human BACE1 shRNA Plasmid |
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| 20-abx958402 | Abbexa |
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 Mouse BACE1 shRNA Plasmid |
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| 20-abx973511 | Abbexa |
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 BACE1 recombinant monoclonal antibody |
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| A5095 | Bimake | 100ul X 3 | 714 EUR |
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Description: A recombinant monoclonal antibody from rabbit against human BACE1 for WB,ELISA |
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) BACE1 Recombinant Protein (Human) |
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| RP002572 | ABM | 100 ug | Ask for price |
) BACE1 Recombinant Protein (Mouse) |
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| RP118622 | ABM | 100 ug | Ask for price |
BACE1 Recombinant Protein (Mouse) |
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| RP118625 | ABM | 100 ug | Ask for price |
) BACE1 Recombinant Protein (Rat) |
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| RP191804 | ABM | 100 ug | Ask for price |
 MitoPT™ TMRE & TMRM Kits |
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| KF17358 | Neuromics | 500 Test | 574.8 EUR |
MitoPT™ TMRE & TMRM Kits |
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| KF17359 | Neuromics | 500 Test | 574.8 EUR |
) HISTAR DETECTION SYSTEM Kits (OKSA11259) |
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| OKSA11259 | Aviva Systems Biology | 50 Tests | 678 EUR |
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Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
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) HISTAR DETECTION SYSTEM Kits (OKSA11260) |
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| OKSA11260 | Aviva Systems Biology | 150 Tests | 1602 EUR |
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Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
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) HISTAR DETECTION SYSTEM Kits (OKSA11261) |
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| OKSA11261 | Aviva Systems Biology | 500 Tests | 2422.8 EUR |
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Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
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 Rat Neural Stem Cell Kits |
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| PC37123 | Neuromics | 1 X 10(6) *minimum order 6 vials* | 1148.4 EUR |
 Rat Bace1/ Beta-secretase 1 ELISA Kit |
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| E0115Ra | Sunlong | 1 Kit | 685.2 EUR |
 Mouse Bace1/ Beta-secretase 1 ELISA Kit |
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| E0159Mo | Sunlong | 1 Kit | 685.2 EUR |
 Human BACE1/ Beta-secretase 1 ELISA Kit |
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| E0249Hu | Sunlong | 1 Kit | 685.2 EUR |
 ELISA Kit) Mouse Bace1(Beta-secretase 1) ELISA Kit |
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| EM0448 | FN Test | 96T | 628.92 EUR |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml |
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 : 96 Wells (OKEH00554)) BACE1 ELISA Kit (Human) : 96 Wells (OKEH00554) |
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| OKEH00554 | Aviva Systems Biology | 96 Wells | 794.4 EUR |
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Description: Description of target: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015];Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.31 ng/mL |
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 Mouse Beta- secretase 1, Bace1 ELISA KIT |
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| ELI-02502m | Lifescience Market | 96 Tests | 1038 EUR |
 Human Beta- secretase 1, BACE1 ELISA KIT |
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| ELI-02503h | Lifescience Market | 96 Tests | 988.8 EUR |
 Bovine Beta- secretase 1, BACE1 ELISA KIT |
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| ELI-02504b | Lifescience Market | 96 Tests | 1113.6 EUR |
 Nori® Porcine 3-Plex ELISA Kits-Interferons |
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| GR224009 | Genorise Scientific | 96-well | 603.6 EUR |
 Nori® Porcine 4-Plex ELISA Kits-Immunoglobulins |
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| GR224017 | Genorise Scientific | 96-well | 654 EUR |
 Nori® Porcine 6-Plex ELISA Kits-BMP |
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| GR224025 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Porcine 6-Plex ELISA Kits-CCL |
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| GR224026 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Porcine 6-Plex ELISA Kits-MMP |
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| GR224027 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Porcine 6-Plex ELISA Kits-TLR |
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| GR224028 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Porcine 6-Plex ELISA Kits-Hormones |
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| GR224029 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Porcine 6-Plex ELISA Kits-SAA |
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| GR224030 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Porcine 6-Plex ELISA Kits-S100 |
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| GR224031 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Porcine 6-Plex ELISA Kits-CXCL |
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| GR224032 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Equine 3-Plex ELISA Kits-Interferons |
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| GR225009 | Genorise Scientific | 96-well | 603.6 EUR |
 Nori® Equine 4-Plex ELISA Kits-Immunoglobulins |
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| GR225017 | Genorise Scientific | 96-well | 654 EUR |
 Nori® Equine 6-Plex ELISA Kits-BMP |
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| GR225025 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Equine 6-Plex ELISA Kits-CCL |
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| GR225026 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Equine 6-Plex ELISA Kits-MMP |
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| GR225027 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Equine 6-Plex ELISA Kits-TLR |
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| GR225028 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Equine 6-Plex ELISA Kits-Hormones |
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| GR225029 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Equine 6-Plex ELISA Kits-SAA |
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| GR225030 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Equine 6-Plex ELISA Kits-S100 |
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| GR225031 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Equine 6-Plex ELISA Kits-CXCL |
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| GR225032 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Canine 3-Plex ELISA Kits-Interferons |
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| GR226009 | Genorise Scientific | 96-well | 603.6 EUR |
 Nori® Canine 4-Plex ELISA Kits-Immunoglobulins |
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| GR226017 | Genorise Scientific | 96-well | 654 EUR |
 Nori® Canine 6-Plex ELISA Kits-BMP |
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| GR226025 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Canine 6-Plex ELISA Kits-CCL |
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| GR226026 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Canine 6-Plex ELISA Kits-MMP |
|||
| GR226027 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Canine 6-Plex ELISA Kits-TLR |
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| GR226028 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Canine 6-Plex ELISA Kits-Hormones |
|||
| GR226029 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Canine 6-Plex ELISA Kits-SAA |
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| GR226030 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Canine 6-Plex ELISA Kits-S100 |
|||
| GR226031 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Canine 6-Plex ELISA Kits-CXCL |
|||
| GR226032 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Bovine 3-Plex ELISA Kits-Interferons |
|||
| GR228009 | Genorise Scientific | 96-well | 603.6 EUR |
 Nori® Bovine 4-Plex ELISA Kits-Immunoglobulins |
|||
| GR228017 | Genorise Scientific | 96-well | 654 EUR |
 Nori® Bovine 6-Plex ELISA Kits-BMP |
|||
| GR228025 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Bovine 6-Plex ELISA Kits-CCL |
|||
| GR228026 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Bovine 6-Plex ELISA Kits-MMP |
|||
| GR228027 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Bovine 6-Plex ELISA Kits-TLR |
|||
| GR228028 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Bovine 6-Plex ELISA Kits-Hormones |
|||
| GR228029 | Genorise Scientific | 96-well | 754.8 EUR |
 Nori® Bovine 6-Plex ELISA Kits-SAA |
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| GR228030 | Genorise Scientific | 96-well | 754.8 EUR |
Treatment with nano extract restored metabolite ranges to regular and means that cytokine ranges have been regulated by nano extract in UC. Conclusion: ASBE nano extract mitigated in opposition to acetic acid-induced colitis in rats, and the underlying mechanism might be attributed to the modulatory results of ASBE on the inflammatory cascades. The applicability of metabolomics developed on this rat mannequin appears to be essential for evaluating the anti-inflammatory mechanisms of latest therapeutics for acute colitis.
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