Biochemical assay and spectroscopic analysis of oxidative/antioxidative parameters in the blood and serum of substance use disorders patients. A methodological comparison study
Substance abuse reminiscent of opioids, hashish, and alcohol causes activation on the immune system and the launch of reactive oxygen species (ROS) into the blood and serum. These substances trigger an impact on oxidant and antioxidant standing in sufferers with substance abuse. Mainly, wide-open to the ROS are lipids and proteins included blood, which suffers peroxidation. In this study, for the first-time differentiation of the results of hashish, alcohol and different artificial substances on blood and serum samples, have been carried out.
For this goal, the stage of the malondialdehyde (MDA) and glutathione (GSH) in serum and pink blood cells, was measured utilizing biochemical assay strategies and Fourier Transform InfraRed spectroscopy (FTIR). The outcomes confirmed, that peroxidation which is dignified as the manufacturing of MDA was elevated for substance use dysfunction (SUD) sufferers (18.01 ± 2.97) in comparison with the management group (10.75 ± 2.28) (p < 0.001) and for antioxidant capability, GSH stage have been considerably elevated for SUD sufferers (p < 0.001).
For the discrimination of protein and lipid area obtained from FTIR spectroscopy, we extracted options by principal part analyze (PCA) of protein (1800 cm-1 to 900 cm-1) and lipid (3200 cm-1 to 2800 cm-1) areas for blood and serum samples collected from sufferers with differing types of SUD and wholesome management (HC) contributors.
For the consideration of lipid oxidation, lipid saturation, lipid desaturation and protein aggregation the peak heights at 1740 cm-1 to 2960 cm-1, 2920 cm-1 to 2960 cm-1, 3012 cm-1 to 2960 cm-1, and 1630 cm-1 to 1650 cm-1 areas have been studied for SUD and HC. Moreover, extra seen modifications have been seen for proteins area, than for lipids. The most discover structural modifications have been noticed in amide II in serum spectra.
Then we categorized protein and lipid area’s PCA outcomes of blood and serum by Linear discriminant analysis (LDA) and Support vector machine (SVM). Confidence of the specificity, sensitivity and accuracy of blood and serum have been obtained as 100%, 100% and 100% individually. This is the first comparative study on the spectrochemical instrument and biochemical assay on SUD. Our outcomes offered 100% discrimination of dysfunction area in comparison with wholesome topics.
Development of a cell-free split-luciferase biochemical assay as a instrument for screening for inhibitors of difficult protein-protein interplay targets
Targeting the interplay of proteins with weak binding affinities or low solubility represents a selected problem for drug screening. The NanoLuc â ® Binary Technology (NanoBiT â ®) was initially developed to detect protein-protein interactions in stay mammalian cells. Here we report the profitable translation of the NanoBit mobile assay right into a biochemical, cell-free format utilizing mammalian cell lysates.
We present that the assay is appropriate for the detection of each robust and weak protein interactions reminiscent of these involving the binding of RAS oncoproteins to both RAF or phosphoinositide 3-kinase (PI3K) effectors respectively, and that it’s also efficient for the study of poorly soluble protein domains reminiscent of the RAS binding area of PI3K. Furthermore, the RAS interplay assay is delicate and responds to each robust and weak RAS inhibitors.
Our information present that the assay is strong, reproducible, cost-effective, and may be tailored for small and large-scale screening approaches. The NanoBit Biochemical Assay provides a horny instrument for drug screening in opposition to difficult protein-protein interplay targets, together with the interplay of RAS with PI3K.
Biochemical characterization of G protein coupling to calcitonin gene-related peptide and adrenomedullin receptors utilizing a local PAGE assay
Calcitonin gene-related peptide (CGRP), adrenomedullin (AM), and adrenomedullin 2/intermedin (AM2/IMD) have overlapping and distinctive features in the nervous and circulatory techniques together with vasodilation, cardioprotection, and ache transmission. Their actions are mediated by the class B calcitonin-like G protein-coupled receptor (CLR), which heterodimerizes with three receptor activity-modifying proteins (RAMP1-3) that decide its peptide ligand selectivity.
How the three agonists and RAMPs modulate CLR binding to transducer proteins stays poorly understood. Here, we biochemically characterised agonist-promoted G protein coupling to every CLR·RAMP complicated. We tailored a local PAGE technique to evaluate the formation and thermostabilities of detergent-solubilized fluorescent protein-tagged CLR·RAMP complexes expressed in mammalian cells.
Read More Here: Human Tumor Necrosis Factor Alpha (TNFA) ELISA Kit. Cat# abx050218. Supplier: abbexa
Addition of agonist and the purified Gs protein surrogate mini-Gs (mGs) yielded a mobility-shifted agonist·CLR·RAMP·mGs quaternary complicated gel band that was delicate to antagonists. Measuring the obvious affinities of the agonists for the mGs-coupled receptors and of mGs for the agonist-occupied receptors revealed that each ligand and RAMP management mGs coupling and outlined how agonist engagement of the CLR extracellular and transmembrane domains impacts transducer recruitment.
 Rat Beta Synuclein ELISA Kit |
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| MBS084873-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Fish Beta Synuclein ELISA Kit |
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| MBS088482-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 Cavy Beta Synuclein ELISA Kit |
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| MBS062248-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 Duck Beta Synuclein ELISA Kit |
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| MBS067810-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 Goat Beta Synuclein ELISA Kit |
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| MBS073084-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 Mouse Beta Synuclein ELISA Kit |
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| MBS105686-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Mouse Beta Synuclein ELISA Kit |
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| MBS105686-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Mouse Beta Synuclein ELISA Kit |
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| MBS105686-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Mouse Beta Synuclein ELISA Kit |
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| MBS105686-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Sheep Beta Synuclein ELISA Kit |
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| MBS048620-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Sheep Beta Synuclein ELISA Kit |
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| MBS048620-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Sheep Beta Synuclein ELISA Kit |
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| MBS048620-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Sheep Beta Synuclein ELISA Kit |
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| MBS048620-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Human Beta Synuclein ELISA Kit |
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| MBS063389-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Human Beta Synuclein ELISA Kit |
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| MBS063389-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Human Beta Synuclein ELISA Kit |
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| MBS063389-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Human Beta Synuclein ELISA Kit |
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| MBS063389-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Camel Beta Synuclein ELISA Kit |
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| MBS049837-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Camel Beta Synuclein ELISA Kit |
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| MBS049837-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Camel Beta Synuclein ELISA Kit |
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| MBS049837-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Camel Beta Synuclein ELISA Kit |
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| MBS049837-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Horse Beta Synuclein ELISA Kit |
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| MBS066789-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Horse Beta Synuclein ELISA Kit |
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| MBS066789-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Horse Beta Synuclein ELISA Kit |
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| MBS066789-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Horse Beta Synuclein ELISA Kit |
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| MBS066789-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Goose Beta Synuclein ELISA Kit |
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| MBS087243-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 Rabbit Beta Synuclein ELISA Kit |
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| MBS091057-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 Canine Beta Synuclein ELISA Kit |
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| MBS060324-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Canine Beta Synuclein ELISA Kit |
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| MBS060324-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Canine Beta Synuclein ELISA Kit |
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| MBS060324-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Canine Beta Synuclein ELISA Kit |
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| MBS060324-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Donkey Beta Synuclein ELISA Kit |
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| MBS062968-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 Bovine Beta Synuclein ELISA Kit |
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| MBS074338-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Bovine Beta Synuclein ELISA Kit |
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| MBS074338-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Bovine Beta Synuclein ELISA Kit |
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| MBS074338-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Bovine Beta Synuclein ELISA Kit |
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| MBS074338-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Pigeon Beta Synuclein ELISA Kit |
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| MBS068095-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 Monkey Beta Synuclein ELISA Kit |
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| MBS080186-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Monkey Beta Synuclein ELISA Kit |
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| MBS080186-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Monkey Beta Synuclein ELISA Kit |
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| MBS080186-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Monkey Beta Synuclein ELISA Kit |
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| MBS080186-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Porcine Beta Synuclein ELISA Kit |
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| MBS097540-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Porcine Beta Synuclein ELISA Kit |
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| MBS097540-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Porcine Beta Synuclein ELISA Kit |
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| MBS097540-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Porcine Beta Synuclein ELISA Kit |
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| MBS097540-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Chicken Beta Synuclein ELISA Kit |
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| MBS052897-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 Hamster Beta Synuclein ELISA Kit |
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| MBS073582-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Hamster Beta Synuclein ELISA Kit |
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| MBS073582-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Hamster Beta Synuclein ELISA Kit |
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| MBS073582-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Hamster Beta Synuclein ELISA Kit |
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| MBS073582-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
 Mouse Beta- synuclein, Sncb ELISA KIT |
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| ELI-29337m | Nova Lifetech | 96tests | 736 EUR |
 Human Beta- synuclein, SNCB ELISA KIT |
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| ELI-13688h | Nova Lifetech | 96tests | 696 EUR |
 ELISA Kit) Human beta synuclein (SNCB) ELISA Kit |
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| MBS7206599-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 5685 EUR |
Human beta synuclein (SNCB) ELISA Kit |
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| MBS7206599-48StripWells | MyBiosource | 48-Strip-Wells | 485 EUR |
Human beta synuclein (SNCB) ELISA Kit |
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| MBS7206599-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3020 EUR |
Human beta synuclein (SNCB) ELISA Kit |
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| MBS7206599-96StripWells | MyBiosource | 96-Strip-Wells | 690 EUR |
 ELISA Kit) Plant Beta Synuclein (SNCB) ELISA Kit |
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| MBS9378862-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 Bovine Beta- synuclein, SNCB ELISA KIT |
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| ELI-53307b | Nova Lifetech | 96tests | 796 EUR |
 ELISA Kit) Rat SNCb(Synuclein beta) ELISA Kit |
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| ELK8982-48T | ELK Biotech | 48T | Ask for price |
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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SNCb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SNCb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SNCb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SNCb in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Rat SNCb(Synuclein beta) ELISA Kit |
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| ELK8982-96T | ELK Biotech | 96T | Ask for price |
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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SNCb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SNCb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SNCb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SNCb in the samples is then determined by comparing the OD of the samples to the standard curve. |
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 ELISA Kit) Rat SNCb (Synuclein beta) ELISA Kit |
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| MBS8807905-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 3130 EUR |
Rat SNCb (Synuclein beta) ELISA Kit |
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| MBS8807905-48StripWells | MyBiosource | 48-Strip-Wells | 350 EUR |
Rat SNCb (Synuclein beta) ELISA Kit |
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| MBS8807905-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 1710 EUR |
Rat SNCb (Synuclein beta) ELISA Kit |
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| MBS8807905-96StripWells | MyBiosource | 96-Strip-Wells | 445 EUR |
 ELISA Kit) Human SNCb(Synuclein beta) ELISA Kit |
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| ELK8964-48T | ELK Biotech | 48T | Ask for price |
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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SNCb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SNCb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SNCb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SNCb in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Human SNCb(Synuclein beta) ELISA Kit |
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| ELK8964-96T | ELK Biotech | 96T | Ask for price |
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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SNCb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SNCb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SNCb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SNCb in the samples is then determined by comparing the OD of the samples to the standard curve. |
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ELISA Kit) Human Synuclein Beta(SNCb)ELISA Kit |
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| QY-E01201 | Qayee Biotechnology | 96T | 433.2 EUR |
 ELISA Kit) Human SNCb (Synuclein beta) ELISA Kit |
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| MBS8807887-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 3130 EUR |
Human SNCb (Synuclein beta) ELISA Kit |
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| MBS8807887-48StripWells | MyBiosource | 48-Strip-Wells | 350 EUR |
Human SNCb (Synuclein beta) ELISA Kit |
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| MBS8807887-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 1710 EUR |
Human SNCb (Synuclein beta) ELISA Kit |
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| MBS8807887-96StripWells | MyBiosource | 96-Strip-Wells | 445 EUR |
 alpha+beta Synuclein |
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| E8ET1609-66 | EnoGene | 100ul | 275 EUR |
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Description: Available in various conjugation types. |
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alpha+beta Synuclein |
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| MBS8533904-01mL | MyBiosource | 0.1mL | 345 EUR |
alpha+beta Synuclein |
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| MBS8533904-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
alpha+beta Synuclein |
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| MBS8533904-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
alpha+beta Synuclein |
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| MBS8533904-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
alpha+beta Synuclein |
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| MBS8533904-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
 beta Synuclein protein |
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| 30R-1010 | Fitzgerald | 100 ug | 219 EUR |
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Description: Purified recombinant Human beta Synuclein protein |
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beta Synuclein protein |
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| 30R-3034 | Fitzgerald | 200 ug | 424.8 EUR |
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Description: Purified recombinant beta Synuclein protein |
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 beta Synuclein Protein |
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| 20-abx260493 | Abbexa |
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 Beta Synuclein Protein |
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| SPR-405B | Stressmarq | 0.1 mg | 382 EUR |
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Description: Human Recombinant Beta Synuclein Protein Monomers |
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Beta Synuclein Protein |
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| SPR-405C | Stressmarq | 2x0.1 mg | 611 EUR |
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Description: Human Recombinant Beta Synuclein Protein Monomers |
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Beta Synuclein Protein |
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| SPR-405E | Stressmarq | 5x0.1 mg | 1240 EUR |
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Description: Human Recombinant Beta Synuclein Protein Monomers |
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Beta Synuclein Protein |
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| SPR-406B | Stressmarq | 0.1 mg | 382 EUR |
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Description: Mouse Recombinant Beta Synuclein Protein Monomers |
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Beta Synuclein Protein |
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| SPR-406C | Stressmarq | 2x0.1 mg | 611 EUR |
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Description: Mouse Recombinant Beta Synuclein Protein Monomers |
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Beta Synuclein Protein |
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| SPR-406E | Stressmarq | 5x0.1 mg | 1240 EUR |
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Description: Mouse Recombinant Beta Synuclein Protein Monomers |
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Beta Synuclein Protein |
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| SPR-457B | Stressmarq | 0.1 mg | 382 EUR |
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Description: Human Recombinant Beta Synuclein Protein Pre-formed Fibrils (Type 1) |
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Beta Synuclein Protein |
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| SPR-457C | Stressmarq | 2x0.1 mg | 611 EUR |
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Description: Human Recombinant Beta Synuclein Protein Pre-formed Fibrils (Type 1) |
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Beta Synuclein Protein |
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| SPR-457E | Stressmarq | 5x0.1 mg | 1240 EUR |
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Description: Human Recombinant Beta Synuclein Protein Pre-formed Fibrils (Type 1) |
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 Beta synuclein antibody |
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| 70R-50407 | Fitzgerald | 100 ul | 242 EUR |
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Description: Purified Polyclonal Beta synuclein antibody |
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 beta Synuclein Antibody |
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| GWB-409C5B | GenWay Biotech | 0.5 ml | Ask for price |
 Beta Synuclein Antibody |
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| MBS5315026-01mL | MyBiosource | 0.1mL | 470 EUR |
Beta Synuclein Antibody |
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| MBS5315026-5x01mL | MyBiosource | 5x0.1mL | 1955 EUR |
 Synuclein, alpha, beta |
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| MBS609677-01mg | MyBiosource | 0.1mg | 875 EUR |
Synuclein, alpha, beta |
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| MBS609677-5x01mg | MyBiosource | 5x0.1mg | 3785 EUR |
Synuclein, alpha, beta |
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| MBS632095-01mL | MyBiosource | 0.1mL | 545 EUR |
Synuclein, alpha, beta |
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| MBS632095-5x01mL | MyBiosource | 5x0.1mL | 2300 EUR |
 Synuclein beta antibody |
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| 70R-30765 | Fitzgerald | 100 ug | 294 EUR |
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Description: Rabbit polyclonal Synuclein beta antibody |
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 Synuclein beta Antibody |
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| AF5324 | Affbiotech | 200ul | 420 EUR |
Synuclein beta Antibody |
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| AF5324-100ul | Affinity Biosciences | 100ul | 280 EUR |
Synuclein beta Antibody |
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| AF5324-200ul | Affinity Biosciences | 200ul | 350 EUR |
Synuclein beta Antibody |
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| MBS9411566-005mL | MyBiosource | 0.05mL | 245 EUR |
Synuclein beta Antibody |
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| MBS9411566-01mL | MyBiosource | 0.1mL | 305 EUR |
Synuclein beta Antibody |
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| MBS9411566-5x01mL | MyBiosource | 5x0.1mL | 1230 EUR |
Synuclein beta Antibody |
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| MBS8511955-005mg | MyBiosource | 0.05mg | 235 EUR |
Synuclein beta Antibody |
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| MBS8511955-01mg | MyBiosource | 0.1mg | 305 EUR |
Synuclein beta Antibody |
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| MBS8511955-01mLAF405M | MyBiosource | 0.1mL(AF405M) | 465 EUR |
Synuclein beta Antibody |
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| MBS8511955-01mLAF546 | MyBiosource | 0.1mL(AF546) | 465 EUR |
Synuclein beta Antibody |
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| MBS8511955-01mLAF750 | MyBiosource | 0.1mL(AF750) | 465 EUR |
Synuclein beta Antibody |
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| MBS851760-01mg | MyBiosource | 0.1mg | 305 EUR |
Synuclein beta Antibody |
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| MBS851760-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 465 EUR |
Synuclein beta Antibody |
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| MBS851760-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 465 EUR |
Synuclein beta Antibody |
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| MBS851760-01mLAF610 | MyBiosource | 0.1mL(AF610) | 465 EUR |
Synuclein beta Antibody |
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| MBS851760-01mLAF635 | MyBiosource | 0.1mL(AF635) | 465 EUR |
Synuclein beta antibody |
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| MBS5303119-01mg | MyBiosource | 0.1mg | 465 EUR |
Using mini-Gsq and -Gsi chimeras, we noticed a coupling rank order of mGs > mGsq > mGsi for every receptor. Last, we demonstrated the physiological relevance of the native gel assays by exhibiting that they’ll predict the cAMP-signaling potencies of AM and AM2/IMD chimeras. These outcomes spotlight the energy of the native PAGE assay for membrane protein biochemistry and present a biochemical basis for understanding the molecular foundation of shared and distinct signaling properties of CGRP, AM, and AM2/IMD.
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