Reconstitution and biochemical assays of an active human histone pre-mRNA 3′-end processing machinery
In animal cells, replication-dependent histone pre-mRNAs are processed on the 3′-end by an endonucleolytic cleavage carried out by the U7 snRNP, a machinery that comprises the U7 snRNA and many protein subunits. Studies on the composition of this machinery and understanding of its position in 3′-end processing had been enormously facilitated by the event of an in vitro system using nuclear extracts from mammalian cells 35 years in the past and later from Drosophila cells.
Most lately, recombinant expression and purification of the elements of the machinery have enabled the total reconstitution of an active machinery and its complicated with a mannequin pre-mRNA substrate, utilizing 13 proteins and 2 RNAs, and the willpower of the construction of this active machinery. This chapter presents protocols for making ready nuclear extracts containing endogenous processing machinery, for assembling semi-recombinant and totally reconstituted machineries, and for histone pre-mRNA 3′-end processing assays with these samples.
Optimization of biochemical sulfide potential (BSP) assay for anaerobic biodegradability evaluation
The anaerobic biodegradability evaluation (biodegradation extent and kinetics) of natural wastes is vital for optimum design and evaluating therapy efficiencies for anaerobic therapy applied sciences. The biochemical sulfide potential (BSP) assay has beforehand demonstrated the benefits of its time effectivity and measurement accuracy for biologically assessing substrate degradability, whereas its software is proscribed by undefined operational parameters. In this research, the BSP assay was additional optimized via a scientific investigation of a vital parameter, inoculum-to-substrate ratio (ISR), and the relevant kinetic mannequin to unravel the potential use of BSP assays for anaerobic waste therapy.
Under two collection of experimental eventualities, the widespread ISR ranges of 0.5-4.0 (based mostly on the standard BMP assay) and excessive ISRs (as little as 0.1) had been studied, by which the benefit of a BSP assay on excessive ISRs was highlighted. Meanwhile, the underlying trigger and mechanism for biodegradability discrepancies beneath totally different ISRs (0.1-6.0) had been additional investigated.
The extracellular polymeric substance (EPS) characterization of residual organics and the two-substrate first-order hydrolysis mannequin analyses revealed that the hydrolysis course of of slowly-biodegradable organics fraction was hindered beneath improper ISR circumstances. Furthermore, the Cone mannequin was evaluated as extra acceptable for biodegradation kinetics evaluation in BSP assays among the many 5 widespread kinetic fashions (i.e., Exponential, Fitzhugh, Cone, Transference, and modified Gompertz fashions). Overall, the outcomes present basic steerage on designing constant BSP assays and put a step ahead in standardizing the BSP assay for anaerobic biodegradability assessments.
Assessment of structural protein expression by FTIR and biochemical assays as biomarkers of metabolites response in gastric and colon most cancers
Colon and gastric cancers are the widespread benign varieties of cancers that are synchronous and metachronous neoplasms. In phrases of the development and progress of the illness, metabolic processes and differentiation in protein buildings have an vital position in for therapy of the illness. In this research we proposed to analyze the metabolic course of and the differentiation of protein secondary construction amongst colon and gastric most cancers in addition to wholesome controls utilizing biochemistry and Fourier Transform InfraRed spectroscopy (FTIR) strategies. For this goal, we measured blood serum of 133 sufferers, which had been performed upon oncology division (45 colon most cancers, 45 gastric most cancers and 43 management people).
The obtained spectroscopic outcomes and biochemical assays confirmed vital discount within the quantity of useful teams in most cancers teams opposite with whole protein measurements and construction of protein variations between colon and gastric cancers. Differentiations had been seen in serum ranges of CEA, CA-125, CA-15-3, CA-19-9 AFP (Alpha fetoprotein) of gastric and colon most cancers sufferers in addition to in amide III and secondly described amide I areas. Our findings recommend that amide I bonds in colon most cancers cells will be useful in prognosis of colon most cancers.
Indeed, our outcomes confirmed that metabolic processes had been greater in gastric most cancers group than in colon most cancers. Hence, FTIR spectroscopy and curve-fitting evaluation of amide I profile will be efficiently utilized as instruments for figuring out quantitative and qualitative adjustments of proteins in human cancerous blood serum. However, what is essential, in PCA evaluation we see, that the scatter plot of PC1 (variability 80%) and PC2 (variability 15%) present that the information associated to the management and two most cancers teams are clustered along with totally different magnitudes and instructions.
High-Throughput Label-Free Biochemical Assays Using Infrared Matrix-Assisted Desorption Electrospray Ionization Mass Spectrometry
Mass spectrometry (MS) can present excessive sensitivity and specificity for biochemical assays with out the requirement of labels, eliminating the danger of assay interference. However, its use had been restricted to lower-throughput assays because of the want for chromatography to beat ion suppression from the pattern matrix.
Direct evaluation with out chromatography has the potential for top throughput if sensitivity is ample regardless of the presence of a matrix. Here, we report and show a novel direct evaluation high-throughput MS system based mostly on infrared matrix-assisted desorption electrospray ionization (IR-MALDESI) that has a possible acquisition price of 33 spectra/s.
 Mouse ADAM10 ELISA Kit |
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| EMA0734 | Abclonal | 96Tests | 625.2 EUR |
 Sheep ADAM10 ELISA Kit |
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| ESA0734 | Abclonal | 96Tests | 625.2 EUR |
 Mouse Adam10 ELISA KIT |
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| ELI-02614m | Lifescience Market | 96 Tests | 1038 EUR |
 Human ADAM10 ELISA KIT |
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| ELI-02618h | Lifescience Market | 96 Tests | 988.8 EUR |
 Human ADAM10 ELISA Kit |
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| ELA-E0766h | Lifescience Market | 96 Tests | 988.8 EUR |
 ADAM10 ELISA KIT|Human |
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| EF001085 | Lifescience Market | 96 Tests | 826.8 EUR |
Human ADAM10 ELISA Kit |
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| EHA0734 | Abclonal | 96Tests | 625.2 EUR |
 Bovine ADAM10 ELISA Kit |
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| EBA0734 | Abclonal | 96Tests | 625.2 EUR |
 Canine ADAM10 ELISA Kit |
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| ECA0734 | Abclonal | 96Tests | 625.2 EUR |
 Bovine ADAM10 ELISA KIT |
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| ELI-02617b | Lifescience Market | 96 Tests | 1113.6 EUR |
 Monkey ADAM10 ELISA Kit |
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| EMKA0734 | Abclonal | 96Tests | 625.2 EUR |
 Rabbit ADAM10 ELISA Kit |
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| ERTA0734 | Abclonal | 96Tests | 625.2 EUR |
 Chicken ADAM10 ELISA Kit |
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| ECKA0734 | Abclonal | 96Tests | 625.2 EUR |
 Porcine ADAM10 ELISA Kit |
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| EPA0734 | Abclonal | 96Tests | 625.2 EUR |
 Anserini ADAM10 ELISA Kit |
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| EAA0734 | Abclonal | 96Tests | 625.2 EUR |
 Guinea Pig ADAM10 ELISA Kit |
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| EGA0734 | Abclonal | 96Tests | 625.2 EUR |
 (OKAN05931)) ADAM10 ELISA Kit (Rat) (OKAN05931) |
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| OKAN05931 | Aviva Systems Biology | 96 Wells | 950.4 EUR |
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Description: Description of target: may act as a disintegrin-metalloprotease related to the reprolysin family [RGD, Feb 2006];Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 27 pg/mL |
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 (OKEH06386)) ADAM10 ELISA Kit (Rat) (OKEH06386) |
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| OKEH06386 | Aviva Systems Biology | 96 Wells | 794.4 EUR |
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Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.082 ng/mL |
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 (OKEH07468)) ADAM10 ELISA Kit (Pig) (OKEH07468) |
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| OKEH07468 | Aviva Systems Biology | 96 Wells | 1310.4 EUR |
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Description: Description of target: ;Species reactivity: Pig;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: |
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 (OKCD06785)) ADAM10 ELISA Kit (Rat) (OKCD06785) |
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| OKCD06785 | Aviva Systems Biology | 96 Wells | 981.6 EUR |
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Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ELISA;Assay info: ;Sensitivity: < 27pg/mL |
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 (OKAN05106)) ADAM10 ELISA Kit (Human) (OKAN05106) |
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| OKAN05106 | Aviva Systems Biology | 96 Wells | 950.4 EUR |
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Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 28 pg/mL |
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 (OKEH06827)) ADAM10 ELISA Kit (Mouse) (OKEH06827) |
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| OKEH06827 | Aviva Systems Biology | 96 Wells | 934.8 EUR |
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Description: Description of target: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero with multiple defects of the developing central nervous system, somites, and cardiovascular system.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.081 ng/mL |
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 (OKCD06783)) ADAM10 ELISA Kit (Human) (OKCD06783) |
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| OKCD06783 | Aviva Systems Biology | 96 Wells | 903.6 EUR |
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Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 28pg/mL |
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 Human ADAM10 GENLISA ELISA |
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| KBH0768 | Krishgen | 1 x 96 wells | 286 EUR |
) OKEH06386-96W - ADAM10 ELISA Kit (Rat) |
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| OKEH06386-96W | Aviva Systems Biology | 96Wells | 525 EUR |
) OKEH07468-96W - ADAM10 ELISA Kit (Pig) |
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| OKEH07468-96W | Aviva Systems Biology | 96Wells | 800 EUR |
) OKCD06785-96W - ADAM10 ELISA Kit (Rat) |
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| OKCD06785-96W | Aviva Systems Biology | 96Wells | 650 EUR |
) OKEH02627-96W - ADAM10 ELISA Kit (Human) |
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| OKEH02627-96W | Aviva Systems Biology | 96Wells | 600 EUR |
) OKEH06827-96W - ADAM10 ELISA Kit (Mouse) |
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| OKEH06827-96W | Aviva Systems Biology | 96Wells | 625 EUR |
) OKCD06783-96W - ADAM10 ELISA Kit (Human) |
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| OKCD06783-96W | Aviva Systems Biology | 96Wells | 600 EUR |
 : 96 Wells (OKEH02627)) ADAM10 ELISA Kit (Human) : 96 Wells (OKEH02627) |
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| OKEH02627 | Aviva Systems Biology | 96 Wells | 888 EUR |
|
Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.045 ng/mL |
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 ADAM10 |
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| CSB-CL001273HU | Cusabio | 10 μg plasmid + 200μl Glycerol | Ask for price |
ADAM10 |
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| E8ET1703-60 | EnoGene | 100ul | 275 EUR |
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Description: Available in various conjugation types. |
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ADAM10 |
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| E8ER1706-97 | EnoGene | 100ul | 275 EUR |
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Description: Available in various conjugation types. |
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ADAM10 |
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| MBS8534462-01mL | MyBiosource | 0.1mL | 345 EUR |
ADAM10 |
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| MBS8534462-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
ADAM10 |
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| MBS8534462-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
ADAM10 |
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| MBS8534462-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
ADAM10 |
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| MBS8534462-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
ADAM10 |
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| MBS8534927-01mL | MyBiosource | 0.1mL | 305 EUR |
ADAM10 |
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| MBS8534927-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
ADAM10 |
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| MBS8534927-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
ADAM10 |
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| MBS8534927-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
ADAM10 |
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| MBS8534927-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
ADAM10 |
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| MBS8535382-01mL | MyBiosource | 0.1mL | 325 EUR |
ADAM10 |
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| MBS8535382-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
ADAM10 |
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| MBS8535382-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
ADAM10 |
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| MBS8535382-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
ADAM10 |
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| MBS8535382-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
ADAM10 |
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| MBS8565293-01mL | MyBiosource | 0.1mL | 345 EUR |
ADAM10 |
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| MBS8565293-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
ADAM10 |
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| MBS8565293-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
ADAM10 |
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| MBS8565293-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
ADAM10 |
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| MBS8565293-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
ADAM10 |
|||
| PR27192 | Neuromics | 2 ug | 97.2 EUR |
ADAM10 |
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| PR27192-B | Neuromics | each | 184.5 EUR |
ADAM10 |
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| pro-476 | ProSpec Tany | 2µg | 60 EUR |
|
Description: Recombinant Human A Disintegrin and Metalloproteinase Domain 10 |
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 Anti-ADAM10/ADAM10 Antibody |
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| PA1535 | BosterBio | 100ug/vial | 154 EUR |
|
Description: Western blot, 0.1-0.5μg/ml, Human |
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 ELISA Kit) Cow ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx513940-96tests | Abbexa | 96 tests | 687.5 EUR |
 ELISA Kit) Pig ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx361975-100g | Abbexa | 100 µg | 687.5 EUR |
Pig ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx361975-96tests | Abbexa | 96 tests | 990 EUR |
 ELISA Kit) Rat ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx576388-96tests | Abbexa | 96 tests | 801.6 EUR |
Rat ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx155141-100g | Abbexa | 100 µg | 2950 EUR |
Rat ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| 20-abx155141 | Abbexa |
|
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Rat ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx155141-50g | Abbexa | 50 µg | 618.75 EUR |
 ELISA Kit) Human ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx250281-96tests | Abbexa | 96 tests | 525 EUR |
 ELISA Kit) Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx513942-96tests | Abbexa | 96 tests | 801.6 EUR |
Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| 20-abx153594 | Abbexa |
|
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Human ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx575998-96tests | Abbexa | 96 tests | 848.4 EUR |
Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| 20-abx258362 | Abbexa |
|
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Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx258362-1mg | Abbexa | 1 mg | 6187.5 EUR |
Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx258362-20g | Abbexa | 20 µg | 3275 EUR |
Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx258362-5g | Abbexa | 5 µg | 687.5 EUR |
Human ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| 20-abx150542 | Abbexa |
|
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 ELISA Kit) Rabbit ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx363169-96tests | Abbexa | 96 tests | 990 EUR |
 ELISA Kit) Monkey ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx360194-96tests | Abbexa | 96 tests | 990 EUR |
 ELISA Kit) Chicken ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx356774-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Chicken ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx356774-596tests | Abbexa | 5 × 96 tests | 662.5 EUR |
Chicken ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit |
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| abx356774-96tests | Abbexa | 96 tests | 990 EUR |
 (OKCD01334)) Adam10 High Sensitivity ELISA Kit (Mouse) (OKCD01334) |
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| OKCD01334 | Aviva Systems Biology | 96 Wells | 1012.8 EUR |
|
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) at '687-Lys-|-Leu-688'. Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 3.7 pg/mL |
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 (OKCD03691)) ADAM10 Chemi-Luminescent ELISA Kit (Rat) (OKCD03691) |
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| OKCD03691 | Aviva Systems Biology | 96 Wells | 1279.2 EUR |
|
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.078 ng/mL |
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 ADAM10 Sf9 |
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| pro-2359 | ProSpec Tany | 1µg | 60 EUR |
|
Description: Recombinant Human A Disintegrin and Metalloproteinase Domain 10, Sf9 |
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) ADAM10 (Human) |
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| GT15078 | Neuromics | 100 ug | 631.2 EUR |
) ADAM10 (Mouse) |
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| GT15079 | Neuromics | 100 ug | 631.2 EUR |
) OKCD01334-96W - Adam10 High Sensitivity ELISA Kit (Mouse) |
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| OKCD01334-96W | Aviva Systems Biology | 96Wells | 675 EUR |
 ADAM10 Peptide |
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| 2051P | ProSci | 0.05 mg | 197.7 EUR |
|
Description: (CT) ADAM10 peptide |
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ADAM10 Peptide |
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| MBS153063-005mg | MyBiosource | 0.05mg | 175 EUR |
ADAM10 Peptide |
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| MBS153063-5x005mg | MyBiosource | 5x0.05mg | 770 EUR |
) OKCD03691-96W - ADAM10 Chemi-Luminescent ELISA Kit (Rat) |
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| OKCD03691-96W | Aviva Systems Biology | 96Wells | 875 EUR |
 ADAM10 Antibody |
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| 36038 | SAB | 100ul | 319 EUR |
ADAM10 Antibody |
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| 36038-100ul | SAB | 100ul | 302.4 EUR |
ADAM10 Antibody |
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| 24044 | SAB | 100ul | 479 EUR |
ADAM10 Antibody |
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| 24044-100ul | SAB | 100ul | 468 EUR |
 ADAM10 Antibody |
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| 3201-100 | Biovision | each | 405.6 EUR |
ADAM10 Antibody |
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| 49473-100ul | SAB | 100ul | 399.6 EUR |
ADAM10 Antibody |
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| 49473-50ul | SAB | 50ul | 286.8 EUR |
 ADAM10 antibody |
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| 70R-11689 | Fitzgerald | 100 ug | 375 EUR |
|
Description: Rabbit polyclonal ADAM10 antibody |
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ADAM10 antibody |
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| 70R-14074 | Fitzgerald | 100 ug | 519 EUR |
|
Description: Affinity purified Rabbit polyclonal ADAM10 antibody |
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ADAM10 Antibody |
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| ABF5294 | Lifescience Market | 100 ug | 525.6 EUR |
ADAM10 Antibody |
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| 2051-002mg | ProSci | 0.02 mg | 206.18 EUR |
|
Description: ADAM10 Antibody: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues. |
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ADAM10 Antibody |
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| 2051-01mg | ProSci | 0.1 mg | 523.7 EUR |
|
Description: ADAM10 Antibody: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues. |
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ADAM10 antibody |
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| 10R-1576 | Fitzgerald | 100 ug | 614.4 EUR |
|
Description: Mouse monoclonal ADAM10 antibody |
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ADAM10 Antibody |
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| 1-CSB-PA000013 | Cusabio |
|
|
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Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000 |
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ADAM10 Antibody |
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| 1-CSB-PA001273LA01HU | Cusabio |
|
|
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Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
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ADAM10 Antibody |
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| AF5294 | Affbiotech | 200ul | 420 EUR |
ADAM10 Antibody |
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| AF5294-100ul | Affinity Biosciences | 100ul | 168 EUR |
|
Description: WB,IHC,IF/ICC,ELISA(peptide) |
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ADAM10 Antibody |
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| AF5294-200ul | Affinity Biosciences | 200ul | 210 EUR |
|
Description: WB,IHC,IF/ICC,ELISA(peptide) |
|||
ADAM10 Antibody |
|||
| CAC07337-100ug | Biomatik Corporation | 100ug | 314 EUR |
ADAM10 Antibody |
|||
| CAC07337-50ug | Biomatik Corporation | 50ug | 199.2 EUR |
ADAM10 Antibody |
|||
| 1-CSB-PA069457 | Cusabio |
|
|
|
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100 |
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ADAM10 Antibody |
|||
| 1-CSB-PA909378 | Cusabio |
|
|
|
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
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ADAM10 Antibody |
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| GWB-ADFF01 | GenWay Biotech | 0.025 mg | Ask for price |
ADAM10 Antibody |
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| E18-5294-1 | EnoGene | 50μg/50μl | 145 EUR |
|
Description: Available in various conjugation types. |
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ADAM10 Antibody |
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| E18-5294-2 | EnoGene | 100μg/100μl | 225 EUR |
|
Description: Available in various conjugation types. |
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ADAM10 Antibody |
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| E036038 | EnoGene | 100μg/100μl | 255 EUR |
|
Description: Available in various conjugation types. |
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ADAM10 Antibody |
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| E300349 | EnoGene | 100ug/200ul | 275 EUR |
|
Description: Available in various conjugation types. |
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ADAM10 Antibody |
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| E38PA5687 | EnoGene | 100ul | 225 EUR |
|
Description: Available in various conjugation types. |
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ADAM10 Antibody |
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| E8RT1018 | EnoGene | 100ul | 225 EUR |
|
Description: Available in various conjugation types. |
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ADAM10 Antibody |
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| E910438 | EnoGene | 100ul | 255 EUR |
|
Description: Available in various conjugation types. |
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ADAM10 Antibody |
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| L0127-100ul | Assay Biotech | 100μl | 217 EUR |
|
Description: ADAM10 Rabbit Polyclonal Antibody |
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ADAM10 Antibody |
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| L0127-50ul | Assay Biotech | 50μl | 143.5 EUR |
|
Description: ADAM10 Rabbit Polyclonal Antibody |
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ADAM10 Antibody |
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| MBS150303-01mg | MyBiosource | 0.1mg | 445 EUR |
ADAM10 Antibody |
|||
| MBS150303-5x01mg | MyBiosource | 5x0.1mg | 1965 EUR |
ADAM10 Antibody |
|||
| MBS5312843-01mg | MyBiosource | 0.1mg | 975 EUR |
ADAM10 Antibody |
|||
| MBS5312843-5x01mg | MyBiosource | 5x0.1mg | 4245 EUR |
ADAM10 Antibody |
|||
| MBS5311216-01mg | MyBiosource | 0.1mg | 505 EUR |
ADAM10 Antibody |
|||
| MBS5311216-5x01mg | MyBiosource | 5x0.1mg | 2235 EUR |
ADAM10 Antibody |
|||
| MBS7115537-005mg | MyBiosource | 0.05mg | 150 EUR |
ADAM10 Antibody |
|||
| MBS7115537-01mg | MyBiosource | 0.1mg | 190 EUR |
ADAM10 Antibody |
|||
| MBS7115537-5x01mg | MyBiosource | 5x0.1mg | 845 EUR |
ADAM10 Antibody |
|||
| MBS7001358-005mg | MyBiosource | 0.05mg | 190 EUR |
ADAM10 Antibody |
|||
| MBS7001358-01mg | MyBiosource | 0.1mg | 270 EUR |
ADAM10 Antibody |
|||
| MBS7001358-5x01mg | MyBiosource | 5x0.1mg | 1205 EUR |
ADAM10 Antibody |
|||
| MBS7124573-005mL | MyBiosource | 0.05mL | 190 EUR |
ADAM10 Antibody |
|||
| MBS7124573-01mL | MyBiosource | 0.1mL | 270 EUR |
ADAM10 Antibody |
|||
| MBS7124573-5x01mL | MyBiosource | 5x0.1mL | 1205 EUR |
ADAM10 Antibody |
|||
| MBS7124574-005mL | MyBiosource | 0.05mL | 190 EUR |
ADAM10 Antibody |
|||
| MBS7124574-01mL | MyBiosource | 0.1mL | 270 EUR |
ADAM10 Antibody |
|||
| MBS7124574-5x01mL | MyBiosource | 5x0.1mL | 1205 EUR |
ADAM10 Antibody |
|||
| MBS9601466-01mL | MyBiosource | 0.1mL | 260 EUR |
ADAM10 Antibody |
|||
| MBS9601466-02mL | MyBiosource | 0.2mL | 305 EUR |
ADAM10 Antibody |
|||
| MBS9601466-5x02mL | MyBiosource | 5x0.2mL | 1220 EUR |
ADAM10 Antibody |
|||
| MBS8583383-01mL | MyBiosource | 0.1mL | 305 EUR |
ADAM10 Antibody |
|||
| MBS8583383-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 465 EUR |
ADAM10 Antibody |
|||
| MBS8583383-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 465 EUR |
ADAM10 Antibody |
|||
| MBS8583383-01mLAF610 | MyBiosource | 0.1mL(AF610) | 465 EUR |
ADAM10 Antibody |
|||
| MBS8583383-01mLAF635 | MyBiosource | 0.1mL(AF635) | 465 EUR |
ADAM10 Antibody |
|||
| MBS851249-01mg | MyBiosource | 0.1mg | 345 EUR |
ADAM10 Antibody |
|||
| MBS851249-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
ADAM10 Antibody |
|||
| MBS851249-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
ADAM10 Antibody |
|||
| MBS851249-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
ADAM10 Antibody |
|||
| MBS851249-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
ADAM10 Antibody |
|||
| MBS8511925-005mg | MyBiosource | 0.05mg | 235 EUR |
ADAM10 Antibody |
|||
| MBS8511925-01mg | MyBiosource | 0.1mg | 305 EUR |
ADAM10 Antibody |
|||
| MBS8511925-01mLAF405M | MyBiosource | 0.1mL(AF405M) | 465 EUR |
ADAM10 Antibody |
|||
| MBS8511925-01mLAF546 | MyBiosource | 0.1mL(AF546) | 465 EUR |
ADAM10 Antibody |
|||
| MBS8511925-01mLAF750 | MyBiosource | 0.1mL(AF750) | 465 EUR |
ADAM10 Antibody |
|||
| MBS9401887-01mL | MyBiosource | 0.1mL | 305 EUR |
ADAM10 Antibody |
|||
| MBS9401887-5x01mL | MyBiosource | 5x0.1mL | 1230 EUR |
ADAM10 Antibody |
|||
| MBS9436218-005mL | MyBiosource | 0.05mL | 325 EUR |
ADAM10 Antibody |
|||
| MBS9436218-01mL | MyBiosource | 0.1mL | 435 EUR |
ADAM10 Antibody |
|||
| MBS9436218-5x01mL | MyBiosource | 5x0.1mL | 1810 EUR |
ADAM10 Antibody |
|||
| MBS9414660-01mL | MyBiosource | 0.1mL | 495 EUR |
ADAM10 Antibody |
|||
| MBS9414660-5x01mL | MyBiosource | 5x0.1mL | 2075 EUR |
 ELISA Kit) Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit |
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| DLR-ADAM10-Ra | DL Develop | 96T | 454 EUR |
|
Description: serum, plasma, tissue homogenates or other biological fluids. |
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Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit |
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| DLR-ADAM10-Ra-48T | DL Develop | 48T | 609.6 EUR |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat A Disintegrin And Metalloprotease 10 (ADAM10) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit |
|||
| DLR-ADAM10-Ra-96T | DL Develop | 96T | 793.2 EUR |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat A Disintegrin And Metalloprotease 10 (ADAM10) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit |
|||
| DL-ADAM10-Ra | DL Develop | 96T | 432 EUR |
|
Description: serum, plasma, tissue homogenates or other biological fluids. |
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Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit |
|||
| EK12430 | SAB | 96Т | 768 EUR |
Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit |
|||
| EKN43153-48T | Biomatik Corporation | 48T | 370.02 EUR |
We present the event of biochemical assays in normal buffers for wild-type isocitrate dehydrogenase 1 (IDH1), diacylglycerol kinase zeta (DGKζ), and p300 histone acetyltransferase (P300) to show the suitability of this technique for a broad vary of high-throughput lead discovery assays. A proof-of-concept pilot display of ∼3k compounds can be proven for IDH1 and in comparison with a beforehand reported fluorescence-based assay. We had been capable of get hold of dependable knowledge at a velocity amenable for high-throughput screening of large-scale compound libraries.
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