Reconstitution and biochemical assays of an active human histone pre-mRNA 3′-end processing machinery

Reconstitution and biochemical assays of an active human histone pre-mRNA 3'-end processing machinery
In animal cells, replication-dependent histone pre-mRNAs are processed on the 3′-end by an endonucleolytic cleavage carried out by the U7 snRNP, a machinery that comprises the U7 snRNA and many protein subunits. Studies on the composition of this machinery and understanding of its position in 3′-end processing had been enormously facilitated by the event of an in vitro system using nuclear extracts from mammalian cells 35 years in the past and later from Drosophila cells.
Most lately, recombinant expression and purification of the elements of the machinery have enabled the total reconstitution of an active machinery and its complicated with a mannequin pre-mRNA substrate, utilizing 13 proteins and 2 RNAs, and the willpower of the construction of this active machinery. This chapter presents protocols for making ready nuclear extracts containing endogenous processing machinery, for assembling semi-recombinant and totally reconstituted machineries, and for histone pre-mRNA 3′-end processing assays with these samples.

Optimization of biochemical sulfide potential (BSP) assay for anaerobic biodegradability evaluation

The anaerobic biodegradability evaluation (biodegradation extent and kinetics) of natural wastes is vital for optimum design and evaluating therapy efficiencies for anaerobic therapy applied sciences. The biochemical sulfide potential (BSP) assay has beforehand demonstrated the benefits of its time effectivity and measurement accuracy for biologically assessing substrate degradability, whereas its software is proscribed by undefined operational parameters. In this research, the BSP assay was additional optimized via a scientific investigation of a vital parameter, inoculum-to-substrate ratio (ISR), and the relevant kinetic mannequin to unravel the potential use of BSP assays for anaerobic waste therapy.
Under two collection of experimental eventualities, the widespread ISR ranges of 0.5-4.0 (based mostly on the standard BMP assay) and excessive ISRs (as little as 0.1) had been studied, by which the benefit of a BSP assay on excessive ISRs was highlighted. Meanwhile, the underlying trigger and mechanism for biodegradability discrepancies beneath totally different ISRs (0.1-6.0) had been additional investigated.
Reconstitution and biochemical assays of an active human histone pre-mRNA 3'-end processing machinery
The extracellular polymeric substance (EPS) characterization of residual organics and the two-substrate first-order hydrolysis mannequin analyses revealed that the hydrolysis course of of slowly-biodegradable organics fraction was hindered beneath improper ISR circumstances. Furthermore, the Cone mannequin was evaluated as extra acceptable for biodegradation kinetics evaluation in BSP assays among the many 5 widespread kinetic fashions (i.e., Exponential, Fitzhugh, Cone, Transference, and modified Gompertz fashions). Overall, the outcomes present basic steerage on designing constant BSP assays and put a step ahead in standardizing the BSP assay for anaerobic biodegradability assessments.

Assessment of structural protein expression by FTIR and biochemical assays as biomarkers of metabolites response in gastric and colon most cancers

Colon and gastric cancers are the widespread benign varieties of cancers that are synchronous and metachronous neoplasms. In phrases of the development and progress of the illness, metabolic processes and differentiation in protein buildings have an vital position in for therapy of the illness. In this research we proposed to analyze the metabolic course of and the differentiation of protein secondary construction amongst colon and gastric most cancers in addition to wholesome controls utilizing biochemistry and Fourier Transform InfraRed spectroscopy (FTIR) strategies. For this goal, we measured blood serum of 133 sufferers, which had been performed upon oncology division (45 colon most cancers, 45 gastric most cancers and 43 management people).
The obtained spectroscopic outcomes and biochemical assays confirmed vital discount within the quantity of useful teams in most cancers teams opposite with whole protein measurements and construction of protein variations between colon and gastric cancers. Differentiations had been seen in serum ranges of CEA, CA-125, CA-15-3, CA-19-9 AFP (Alpha fetoprotein) of gastric and colon most cancers sufferers in addition to in amide III and secondly described amide I areas. Our findings recommend that amide I bonds in colon most cancers cells will be useful in prognosis of colon most cancers.
Indeed, our outcomes confirmed that metabolic processes had been greater in gastric most cancers group than in colon most cancers. Hence, FTIR spectroscopy and curve-fitting evaluation of amide I profile will be efficiently utilized as instruments for figuring out quantitative and qualitative adjustments of proteins in human cancerous blood serum. However, what is essential, in PCA evaluation we see, that the scatter plot of PC1 (variability 80%) and PC2 (variability 15%) present that the information associated to the management and two most cancers teams are clustered along with totally different magnitudes and instructions.

High-Throughput Label-Free Biochemical Assays Using Infrared Matrix-Assisted Desorption Electrospray Ionization Mass Spectrometry

Mass spectrometry (MS) can present excessive sensitivity and specificity for biochemical assays with out the requirement of labels, eliminating the danger of assay interference. However, its use had been restricted to lower-throughput assays because of the want for chromatography to beat ion suppression from the pattern matrix.
Direct evaluation with out chromatography has the potential for top throughput if sensitivity is ample regardless of the presence of a matrix. Here, we report and show a novel direct evaluation high-throughput MS system based mostly on infrared matrix-assisted desorption electrospray ionization (IR-MALDESI) that has a possible acquisition price of 33 spectra/s.

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RD-ADAM10-Hu-48Tests Reddot Biotech 48 Tests 573.6 EUR

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RD-ADAM10-Hu-96Tests Reddot Biotech 96 Tests 794.4 EUR

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RD-ADAM10-Ra-48Tests Reddot Biotech 48 Tests 613.2 EUR

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RD-ADAM10-Ra-96Tests Reddot Biotech 96 Tests 850.8 EUR

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Hu-48Tests Reddot Biotech 48 Tests 600 EUR

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Hu-96Tests Reddot Biotech 96 Tests 830.4 EUR

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Ra-48Tests Reddot Biotech 48 Tests 640.8 EUR

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Ra-96Tests Reddot Biotech 96 Tests 890.4 EUR

Adam10/ Rat Adam10 ELISA Kit

ELI-02616r Lifescience Market 96 Tests 1063.2 EUR

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 Alpha Diagnostics 1 242.4 EUR

Anti-ADAM10/ADAM10 Antibody

PA1535 BosterBio 100ug/vial 352.8 EUR

ADAM10

PR27192 Neuromics 2 ug 229.2 EUR

Rabbit ADAM10 ELISA Kit

ERTA0734 Abclonal 96Tests 625.2 EUR

Rat ADAM10 ELISA Kit

ERA0734 Abclonal 96Tests 625.2 EUR

Mouse ADAM10 ELISA Kit

EMA0734 Abclonal 96Tests 625.2 EUR

Monkey ADAM10 ELISA Kit

EMKA0734 Abclonal 96Tests 625.2 EUR

Porcine ADAM10 ELISA Kit

EPA0734 Abclonal 96Tests 625.2 EUR

Human ADAM10 ELISA Kit

EHA0734 Abclonal 96Tests 625.2 EUR

Goat ADAM10 ELISA Kit

EGTA0734 Abclonal 96Tests 625.2 EUR

Canine ADAM10 ELISA Kit

ECA0734 Abclonal 96Tests 625.2 EUR

Chicken ADAM10 ELISA Kit

ECKA0734 Abclonal 96Tests 625.2 EUR

Anserini ADAM10 ELISA Kit

EAA0734 Abclonal 96Tests 625.2 EUR

Bovine ADAM10 ELISA Kit

EBA0734 Abclonal 96Tests 625.2 EUR

ADAM10 ELISA KIT|Human

EF001085 Lifescience Market 96 Tests 826.8 EUR

Sheep ADAM10 ELISA Kit

ESA0734 Abclonal 96Tests 625.2 EUR

Mouse Adam10 ELISA KIT

ELI-02614m Lifescience Market 96 Tests 1038 EUR

Bovine ADAM10 ELISA KIT

ELI-02617b Lifescience Market 96 Tests 1113.6 EUR

Human ADAM10 ELISA KIT

ELI-02618h Lifescience Market 96 Tests 988.8 EUR

Human ADAM10 ELISA Kit

ELA-E0766h Lifescience Market 96 Tests 988.8 EUR

ADAM10 antibody

70R-11689 Fitzgerald 100 ug 536.4 EUR
Description: Rabbit polyclonal ADAM10 antibody

ADAM10 antibody

70R-14074 Fitzgerald 100 ug 386.4 EUR
Description: Affinity purified Rabbit polyclonal ADAM10 antibody

ADAM10 Antibody

3201-100 Biovision 405.6 EUR

ADAM10 Antibody

49473-100ul SAB 100ul 399.6 EUR

ADAM10 Antibody

49473-50ul SAB 50ul 286.8 EUR

ADAM10 Antibody

2051-002mg ProSci 0.02 mg 206.18 EUR
Description: ADAM10 Antibody: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

ADAM10 Antibody

2051-01mg ProSci 0.1 mg 523.7 EUR
Description: ADAM10 Antibody: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

ADAM10 Peptide

2051P ProSci 0.05 mg 197.7 EUR
Description: (CT) ADAM10 peptide

ADAM10 Antibody

36038-100ul SAB 100ul 302.4 EUR

ADAM10 antibody

10R-1576 Fitzgerald 100 ug 614.4 EUR
Description: Mouse monoclonal ADAM10 antibody

ADAM10 Antibody

24044-100ul SAB 100ul 468 EUR

ADAM10 Antibody

ABF5294 Lifescience Market 100 ug 525.6 EUR

ADAM10 siRNA

20-abx906699 Abbexa
  • 661.20 EUR
  • 878.40 EUR
  • 15 nmol
  • 30 nmol

ADAM10 siRNA

20-abx906700 Abbexa
  • 661.20 EUR
  • 878.40 EUR
  • 15 nmol
  • 30 nmol

ADAM10 Antibody

1-CSB-PA000013 Cusabio
  • 266.40 EUR
  • 234.00 EUR
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

ADAM10 Antibody

1-CSB-PA001273LA01HU Cusabio
  • 380.40 EUR
  • 402.00 EUR
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

ADAM10 Antibody

1-CSB-PA909378 Cusabio
  • 380.40 EUR
  • 292.80 EUR
  • 100ul
  • 50ul
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

ADAM10 Antibody

1-CSB-PA069457 Cusabio
  • 380.40 EUR
  • 292.80 EUR
  • 100ul
  • 50ul
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

ADAM10 Antibody

AF5294 Affbiotech 200ul 420 EUR

ADAM10 (Human)

GT15078 Neuromics 100 ug 631.2 EUR

ADAM10 (Mouse)

GT15079 Neuromics 100 ug 631.2 EUR

ADAM10 Antibody

R30611 NSJ Bioreagents 100 ug 419 EUR

ADAM10 Antibody

RQ4035 NSJ Bioreagents 100 ug 419 EUR

ADAM10 Antibody

RQ4703 NSJ Bioreagents 100ul 419 EUR

ADAM10 ELISA Kit (Rat) (OKAN05931)

OKAN05931 Aviva Systems Biology 96 Wells 950.4 EUR
Description: Description of target: may act as a disintegrin-metalloprotease related to the reprolysin family [RGD, Feb 2006];Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 27 pg/mL

ADAM10 ELISA Kit (Rat) (OKEH06386)

OKEH06386 Aviva Systems Biology 96 Wells 794.4 EUR
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.082 ng/mL

ADAM10 ELISA Kit (Mouse) (OKEH06827)

OKEH06827 Aviva Systems Biology 96 Wells 934.8 EUR
Description: Description of target: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero with multiple defects of the developing central nervous system, somites, and cardiovascular system.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.081 ng/mL

ADAM10 ELISA Kit (Pig) (OKEH07468)

OKEH07468 Aviva Systems Biology 96 Wells 1310.4 EUR
Description: Description of target: ;Species reactivity: Pig;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity:

ADAM10 ELISA Kit (Human) (OKAN05106)

OKAN05106 Aviva Systems Biology 96 Wells 950.4 EUR
Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 28 pg/mL

ADAM10 ELISA Kit (Human) (OKCD06783)

OKCD06783 Aviva Systems Biology 96 Wells 903.6 EUR
Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 28pg/mL

ADAM10 ELISA Kit (Rat) (OKCD06785)

OKCD06785 Aviva Systems Biology 96 Wells 981.6 EUR
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ELISA;Assay info: ;Sensitivity: < 27pg/mL

Guinea Pig ADAM10 ELISA Kit

EGA0734 Abclonal 96Tests 625.2 EUR

ADAM10 Rabbit pAb

A10438-100ul Abclonal 100 ul 369.6 EUR

ADAM10 Rabbit pAb

A10438-200ul Abclonal 200 ul 550.8 EUR

ADAM10 Rabbit pAb

A10438-20ul Abclonal 20 ul 219.6 EUR

ADAM10 Rabbit pAb

A10438-50ul Abclonal 50 ul 267.6 EUR

Anti-ADAM10 Antibody

A00566-1 BosterBio 100ug/vial 352.8 EUR

ADAM10 Polyclonal Antibody

ABP50011-003ml Abbkine 0.03ml 189.6 EUR
Description: A polyclonal antibody for detection of ADAM10 from Human. This ADAM10 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ADAM10 at AA range: 170-250

ADAM10 Polyclonal Antibody

ABP50011-01ml Abbkine 0.1ml 346.8 EUR
Description: A polyclonal antibody for detection of ADAM10 from Human. This ADAM10 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ADAM10 at AA range: 170-250

ADAM10 Polyclonal Antibody

ABP50011-02ml Abbkine 0.2ml 496.8 EUR
Description: A polyclonal antibody for detection of ADAM10 from Human. This ADAM10 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ADAM10 at AA range: 170-250

Human ADAM10 Protein

20-abx262305 Abbexa
  • 393.60 EUR
  • 7108.80 EUR
  • 276.00 EUR
  • 10 ug
  • 1 mg
  • 2 µg

ADAM10 Conjugated Antibody

C36038 SAB 100ul 476.4 EUR

ADAM10 Conjugated Antibody

C49473 SAB 100ul 476.4 EUR

ADAM10 Blocking Peptide

AF5294-BP Affbiotech 1mg 234 EUR

ADAM10 cloning plasmid

CSB-CL001273HU-10ug Cusabio 10ug 451.2 EUR
Description: A cloning plasmid for the ADAM10 gene.

ADAM10 Polyclonal Antibody

ES1010-100ul ELK Biotech 100ul 334.8 EUR
Description: A Rabbit Polyclonal antibody against ADAM10 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

ADAM10 Polyclonal Antibody

ES1010-50ul ELK Biotech 50ul 248.4 EUR
Description: A Rabbit Polyclonal antibody against ADAM10 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

ADAM10 (Human), CF

PR15036CF Neuromics 20 ug 631.2 EUR

Anti-ADAM10 antibody

STJ112469 St John's Laboratory 100 µl 332.4 EUR
Description: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.

Anti-ADAM10 antibody

STJ90014 St John's Laboratory 200 µl 236.4 EUR
Description: Rabbit polyclonal to ADAM10.

ADAM10 Polyclonal Antibody

A58010 EpiGentek
  • 684.66 EUR
  • Ask for price
  • Ask for price
  • 423.50 EUR
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

ADAM10 Polyclonal Antibody

A71895 EpiGentek
  • 302.50 EUR
  • 423.50 EUR
  • 50 ul
  • 100 ul

ADAM10 Polyclonal Antibody

A58010-020 EpiGentek 20 ul Ask for price

ADAM10 Polyclonal Antibody

A58010-050 EpiGentek 50 ul Ask for price

ADAM10 Polyclonal Antibody

A58010-100 EpiGentek 100 ul 423.5 EUR

ADAM10 Polyclonal Antibody

A71895-050 EpiGentek 50 ul 302.5 EUR

ADAM10 Polyclonal Antibody

A71895-100 EpiGentek 100 ul 423.5 EUR

Basic Cytotoxicity Test Kits

CT17001 Neuromics 125 Tests 490.8 EUR

Basic Cytotoxicity Test Kits

CT17002 Neuromics 250 Tests 780 EUR

Total Cytotoxicity Test Kits

CT17003 Neuromics 125 Tests 615.6 EUR

Total Cytotoxicity Test Kits

CT17004 Neuromics 250 Tests 975.6 EUR

Necrosis vs Apoptosis Kits

KF17371 Neuromics 50-100 Tests 522 EUR

Necrosis vs Apoptosis Kits

KF17372 Neuromics 100-200 Tests 921.6 EUR

T&A Cloning Kits

FYC001-20P Yeastern Biotech 1 vial Ask for price

Pipette Stand Starter Kits

P7700-S1 Benchmark Scientific 1 PC 609.4 EUR

Pipette Stand Starter Kits

P7700-S2 Benchmark Scientific 1 PC 631.32 EUR

Sealed Vials Calibration Kits

2659505 Scientific Laboratory Supplies EACH 814.6 EUR

Carcass Sponge Sampling Kits

SWA1038 Scientific Laboratory Supplies PK95 332.88 EUR

ADAM10 recombinant monoclonal antibody

A5298 Bimake 100ul X 3 714 EUR
Description: A recombinant monoclonal antibody from rabbit against human ADAM10 for WB,ELISA

Human ADAM10 shRNA Plasmid

20-abx950066 Abbexa
  • 961.20 EUR
  • 1345.20 EUR
  • 150 µg
  • 300 µg

ADAM10 Antibody, HRP conjugated

1-CSB-PA001273LB01HU Cusabio
  • 380.40 EUR
  • 402.00 EUR
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ADAM10 Antibody, FITC conjugated

1-CSB-PA001273LC01HU Cusabio
  • 380.40 EUR
  • 402.00 EUR
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ADAM10 Antibody, Biotin conjugated

1-CSB-PA001273LD01HU Cusabio
  • 380.40 EUR
  • 402.00 EUR
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Mouse ADAM10 shRNA Plasmid

20-abx969028 Abbexa
  • 961.20 EUR
  • 1345.20 EUR
  • 150 µg
  • 300 µg

ADAM10 Recombinant Protein (Human)

RP036424 ABM 100 ug Ask for price

ADAM10 Recombinant Protein (Mouse)

RP114161 ABM 100 ug Ask for price

HISTAR DETECTION SYSTEM Kits (OKSA11259)

OKSA11259 Aviva Systems Biology 50 Tests 678 EUR
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11260)

OKSA11260 Aviva Systems Biology 150 Tests 1602 EUR
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11261)

OKSA11261 Aviva Systems Biology 500 Tests 2422.8 EUR
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

MitoPT™ TMRE & TMRM Kits

KF17358 Neuromics 500 Test 574.8 EUR

MitoPT™ TMRE & TMRM Kits

KF17359 Neuromics 500 Test 574.8 EUR

Rat Neural Stem Cell Kits

PC37123 Neuromics 1 X 10(6) *minimum order 6 vials* 1148.4 EUR

ADAM10 ELISA Kit (Human) : 96 Wells (OKEH02627)

OKEH02627 Aviva Systems Biology 96 Wells 888 EUR
Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.045 ng/mL

ADAM10 Chemi-Luminescent ELISA Kit (Rat) (OKCD03691)

OKCD03691 Aviva Systems Biology 96 Wells 1279.2 EUR
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.078 ng/mL

Adam10 High Sensitivity ELISA Kit (Mouse) (OKCD01334)

OKCD01334 Aviva Systems Biology 96 Wells 1012.8 EUR
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) at '687-Lys-|-Leu-688'. Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 3.7 pg/mL

Nori® Porcine 3-Plex ELISA Kits-Interferons

GR224009 Genorise Scientific 96-well 603.6 EUR

Nori® Porcine 4-Plex ELISA Kits-Immunoglobulins

GR224017 Genorise Scientific 96-well 654 EUR

Nori® Porcine 6-Plex ELISA Kits-BMP

GR224025 Genorise Scientific 96-well 754.8 EUR

Nori® Porcine 6-Plex ELISA Kits-CCL

GR224026 Genorise Scientific 96-well 754.8 EUR

Nori® Porcine 6-Plex ELISA Kits-MMP

GR224027 Genorise Scientific 96-well 754.8 EUR

Nori® Porcine 6-Plex ELISA Kits-TLR

GR224028 Genorise Scientific 96-well 754.8 EUR

Nori® Porcine 6-Plex ELISA Kits-Hormones

GR224029 Genorise Scientific 96-well 754.8 EUR

Nori® Porcine 6-Plex ELISA Kits-SAA

GR224030 Genorise Scientific 96-well 754.8 EUR

Nori® Porcine 6-Plex ELISA Kits-S100

GR224031 Genorise Scientific 96-well 754.8 EUR

Nori® Porcine 6-Plex ELISA Kits-CXCL

GR224032 Genorise Scientific 96-well 754.8 EUR

Nori® Equine 3-Plex ELISA Kits-Interferons

GR225009 Genorise Scientific 96-well 603.6 EUR

Nori® Equine 4-Plex ELISA Kits-Immunoglobulins

GR225017 Genorise Scientific 96-well 654 EUR

Nori® Equine 6-Plex ELISA Kits-BMP

GR225025 Genorise Scientific 96-well 754.8 EUR

Nori® Equine 6-Plex ELISA Kits-CCL

GR225026 Genorise Scientific 96-well 754.8 EUR

Nori® Equine 6-Plex ELISA Kits-MMP

GR225027 Genorise Scientific 96-well 754.8 EUR

Nori® Equine 6-Plex ELISA Kits-TLR

GR225028 Genorise Scientific 96-well 754.8 EUR

Nori® Equine 6-Plex ELISA Kits-Hormones

GR225029 Genorise Scientific 96-well 754.8 EUR

Nori® Equine 6-Plex ELISA Kits-SAA

GR225030 Genorise Scientific 96-well 754.8 EUR

Nori® Equine 6-Plex ELISA Kits-S100

GR225031 Genorise Scientific 96-well 754.8 EUR

Nori® Equine 6-Plex ELISA Kits-CXCL

GR225032 Genorise Scientific 96-well 754.8 EUR

Nori® Canine 3-Plex ELISA Kits-Interferons

GR226009 Genorise Scientific 96-well 603.6 EUR

Nori® Canine 4-Plex ELISA Kits-Immunoglobulins

GR226017 Genorise Scientific 96-well 654 EUR

Nori® Canine 6-Plex ELISA Kits-BMP

GR226025 Genorise Scientific 96-well 754.8 EUR

Nori® Canine 6-Plex ELISA Kits-CCL

GR226026 Genorise Scientific 96-well 754.8 EUR

Nori® Canine 6-Plex ELISA Kits-MMP

GR226027 Genorise Scientific 96-well 754.8 EUR

Nori® Canine 6-Plex ELISA Kits-TLR

GR226028 Genorise Scientific 96-well 754.8 EUR

Nori® Canine 6-Plex ELISA Kits-Hormones

GR226029 Genorise Scientific 96-well 754.8 EUR

Nori® Canine 6-Plex ELISA Kits-SAA

GR226030 Genorise Scientific 96-well 754.8 EUR

Nori® Canine 6-Plex ELISA Kits-S100

GR226031 Genorise Scientific 96-well 754.8 EUR

Nori® Canine 6-Plex ELISA Kits-CXCL

GR226032 Genorise Scientific 96-well 754.8 EUR

Nori® Bovine 3-Plex ELISA Kits-Interferons

GR228009 Genorise Scientific 96-well 603.6 EUR

Nori® Bovine 4-Plex ELISA Kits-Immunoglobulins

GR228017 Genorise Scientific 96-well 654 EUR

Nori® Bovine 6-Plex ELISA Kits-BMP

GR228025 Genorise Scientific 96-well 754.8 EUR

Nori® Bovine 6-Plex ELISA Kits-CCL

GR228026 Genorise Scientific 96-well 754.8 EUR

Nori® Bovine 6-Plex ELISA Kits-MMP

GR228027 Genorise Scientific 96-well 754.8 EUR

Nori® Bovine 6-Plex ELISA Kits-TLR

GR228028 Genorise Scientific 96-well 754.8 EUR

Nori® Bovine 6-Plex ELISA Kits-Hormones

GR228029 Genorise Scientific 96-well 754.8 EUR

Nori® Bovine 6-Plex ELISA Kits-SAA

GR228030 Genorise Scientific 96-well 754.8 EUR

Nori® Bovine 6-Plex ELISA Kits-S100

GR228031 Genorise Scientific 96-well 754.8 EUR

Nori® Bovine 6-Plex ELISA Kits-CXCL

GR228032 Genorise Scientific 96-well 754.8 EUR

ADAM10, Human Recombinant Protein, E.Coli

CG1002-002 GenDepot 2 ug 271.2 EUR

ADAM10, Human Recombinant Protein, E.Coli

CG1002-010 GenDepot 10 ug 360 EUR

Anti-ADAM10 Rabbit Monoclonal Antibody

M00566 BosterBio 100ug/vial 476.4 EUR
Description: Rabbit Monoclonal ADAM10 Antibody. Validated in IP, WB and tested in Human, Mouse, Rat.

ADAM10 ORF Vector (Human) (pORF)

ORF012142 ABM 1.0 ug DNA 424.8 EUR

Adam10 ORF Vector (Mouse) (pORF)

ORF038055 ABM 1.0 ug DNA 607.2 EUR

ADAM10 Polyclonal Antibody, Biotin Conjugated

A58011 EpiGentek
  • 684.66 EUR
  • 302.50 EUR
  • 423.50 EUR
  • 100 µg
  • 50 ul
  • 100 ul

ADAM10 Polyclonal Antibody, FITC Conjugated

A58012 EpiGentek
  • 684.66 EUR
  • 302.50 EUR
  • 423.50 EUR
  • 100 µg
  • 50 ul
  • 100 ul

ADAM10 Polyclonal Antibody, HRP Conjugated

A58013 EpiGentek
  • 684.66 EUR
  • 302.50 EUR
  • 423.50 EUR
  • 100 µg
  • 50 ul
  • 100 ul

ADAM10 Polyclonal Antibody, Biotin Conjugated

A58011-050 EpiGentek 50 ul 302.5 EUR

ADAM10 Polyclonal Antibody, Biotin Conjugated

A58011-100 EpiGentek 100 ul 423.5 EUR

ADAM10 Polyclonal Antibody, FITC Conjugated

A58012-050 EpiGentek 50 ul 302.5 EUR

ADAM10 Polyclonal Antibody, FITC Conjugated

A58012-100 EpiGentek 100 ul 423.5 EUR

ADAM10 Polyclonal Antibody, HRP Conjugated

A58013-050 EpiGentek 50 ul 302.5 EUR

ADAM10 Polyclonal Antibody, HRP Conjugated

A58013-100 EpiGentek 100 ul 423.5 EUR

CellQuanti-Blue Cell Viability Assay Kits

CQBL-05K BioAssay Systems 5000 265.2 EUR
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 5000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-Blue Cell Viability Assay Kits

CQBL-10K BioAssay Systems 10000 408 EUR
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 10000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.
We present the event of biochemical assays in normal buffers for wild-type isocitrate dehydrogenase 1 (IDH1), diacylglycerol kinase zeta (DGKζ), and p300 histone acetyltransferase (P300) to show the suitability of this technique for a broad vary of high-throughput lead discovery assays. A proof-of-concept pilot display of ∼3k compounds can be proven for IDH1 and in comparison with a beforehand reported fluorescence-based assay. We had been capable of get hold of dependable knowledge at a velocity amenable for high-throughput screening of large-scale compound libraries.

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