Reconstitution and biochemical assays of an active human histone pre-mRNA 3′-end processing machinery

Reconstitution and biochemical assays of an active human histone pre-mRNA 3'-end processing machinery
In animal cells, replication-dependent histone pre-mRNAs are processed on the 3′-end by an endonucleolytic cleavage carried out by the U7 snRNP, a machinery that comprises the U7 snRNA and many protein subunits. Studies on the composition of this machinery and understanding of its position in 3′-end processing had been enormously facilitated by the event of an in vitro system using nuclear extracts from mammalian cells 35 years in the past and later from Drosophila cells.
Most lately, recombinant expression and purification of the elements of the machinery have enabled the total reconstitution of an active machinery and its complicated with a mannequin pre-mRNA substrate, utilizing 13 proteins and 2 RNAs, and the willpower of the construction of this active machinery. This chapter presents protocols for making ready nuclear extracts containing endogenous processing machinery, for assembling semi-recombinant and totally reconstituted machineries, and for histone pre-mRNA 3′-end processing assays with these samples.

Optimization of biochemical sulfide potential (BSP) assay for anaerobic biodegradability evaluation

The anaerobic biodegradability evaluation (biodegradation extent and kinetics) of natural wastes is vital for optimum design and evaluating therapy efficiencies for anaerobic therapy applied sciences. The biochemical sulfide potential (BSP) assay has beforehand demonstrated the benefits of its time effectivity and measurement accuracy for biologically assessing substrate degradability, whereas its software is proscribed by undefined operational parameters. In this research, the BSP assay was additional optimized via a scientific investigation of a vital parameter, inoculum-to-substrate ratio (ISR), and the relevant kinetic mannequin to unravel the potential use of BSP assays for anaerobic waste therapy.
Under two collection of experimental eventualities, the widespread ISR ranges of 0.5-4.0 (based mostly on the standard BMP assay) and excessive ISRs (as little as 0.1) had been studied, by which the benefit of a BSP assay on excessive ISRs was highlighted. Meanwhile, the underlying trigger and mechanism for biodegradability discrepancies beneath totally different ISRs (0.1-6.0) had been additional investigated.
Reconstitution and biochemical assays of an active human histone pre-mRNA 3'-end processing machinery
The extracellular polymeric substance (EPS) characterization of residual organics and the two-substrate first-order hydrolysis mannequin analyses revealed that the hydrolysis course of of slowly-biodegradable organics fraction was hindered beneath improper ISR circumstances. Furthermore, the Cone mannequin was evaluated as extra acceptable for biodegradation kinetics evaluation in BSP assays among the many 5 widespread kinetic fashions (i.e., Exponential, Fitzhugh, Cone, Transference, and modified Gompertz fashions). Overall, the outcomes present basic steerage on designing constant BSP assays and put a step ahead in standardizing the BSP assay for anaerobic biodegradability assessments.

Assessment of structural protein expression by FTIR and biochemical assays as biomarkers of metabolites response in gastric and colon most cancers

Colon and gastric cancers are the widespread benign varieties of cancers that are synchronous and metachronous neoplasms. In phrases of the development and progress of the illness, metabolic processes and differentiation in protein buildings have an vital position in for therapy of the illness. In this research we proposed to analyze the metabolic course of and the differentiation of protein secondary construction amongst colon and gastric most cancers in addition to wholesome controls utilizing biochemistry and Fourier Transform InfraRed spectroscopy (FTIR) strategies. For this goal, we measured blood serum of 133 sufferers, which had been performed upon oncology division (45 colon most cancers, 45 gastric most cancers and 43 management people).
The obtained spectroscopic outcomes and biochemical assays confirmed vital discount within the quantity of useful teams in most cancers teams opposite with whole protein measurements and construction of protein variations between colon and gastric cancers. Differentiations had been seen in serum ranges of CEA, CA-125, CA-15-3, CA-19-9 AFP (Alpha fetoprotein) of gastric and colon most cancers sufferers in addition to in amide III and secondly described amide I areas. Our findings recommend that amide I bonds in colon most cancers cells will be useful in prognosis of colon most cancers.
Indeed, our outcomes confirmed that metabolic processes had been greater in gastric most cancers group than in colon most cancers. Hence, FTIR spectroscopy and curve-fitting evaluation of amide I profile will be efficiently utilized as instruments for figuring out quantitative and qualitative adjustments of proteins in human cancerous blood serum. However, what is essential, in PCA evaluation we see, that the scatter plot of PC1 (variability 80%) and PC2 (variability 15%) present that the information associated to the management and two most cancers teams are clustered along with totally different magnitudes and instructions.

High-Throughput Label-Free Biochemical Assays Using Infrared Matrix-Assisted Desorption Electrospray Ionization Mass Spectrometry

Mass spectrometry (MS) can present excessive sensitivity and specificity for biochemical assays with out the requirement of labels, eliminating the danger of assay interference. However, its use had been restricted to lower-throughput assays because of the want for chromatography to beat ion suppression from the pattern matrix.
Direct evaluation with out chromatography has the potential for top throughput if sensitivity is ample regardless of the presence of a matrix. Here, we report and show a novel direct evaluation high-throughput MS system based mostly on infrared matrix-assisted desorption electrospray ionization (IR-MALDESI) that has a possible acquisition price of 33 spectra/s.

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Hu-48Tests 48 Tests
EUR 500

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Hu-96Tests 96 Tests
EUR 692

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Ra-48Tests 48 Tests
EUR 534

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Ra-96Tests 96 Tests
EUR 742

Adam10/ Rat Adam10 ELISA Kit

ELI-02616r 96 Tests
EUR 886

Anti-ADAM10/ADAM10 Antibody

PA1535 100ug/vial
EUR 294

ADAM10

PR27192 2 ug
EUR 191

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 1
EUR 202

Pipette Stand Starter Kits

P7700-S1 1 PC
EUR 507.83

Pipette Stand Starter Kits

P7700-S2 1 PC
EUR 526.1

T&A Cloning Kits

FYC001-20P 1 vial Ask for price

Basic Cytotoxicity Test Kits

CT17001 125 Tests
EUR 409

Basic Cytotoxicity Test Kits

CT17002 250 Tests
EUR 650

Total Cytotoxicity Test Kits

CT17003 125 Tests
EUR 513

Total Cytotoxicity Test Kits

CT17004 250 Tests
EUR 813

Necrosis vs Apoptosis Kits

KF17371 50-100 Tests
EUR 435

Necrosis vs Apoptosis Kits

KF17372 100-200 Tests
EUR 768

Human ADAM10 ELISA Kit

ELA-E0766h 96 Tests
EUR 824

Mouse Adam10 ELISA KIT

ELI-02614m 96 Tests
EUR 865

Bovine ADAM10 ELISA KIT

ELI-02617b 96 Tests
EUR 928

Human ADAM10 ELISA KIT

ELI-02618h 96 Tests
EUR 824

ADAM10 ELISA KIT|Human

EF001085 96 Tests
EUR 689

Sheep ADAM10 ELISA Kit

ESA0734 96Tests
EUR 521

Mouse ADAM10 ELISA Kit

EMA0734 96Tests
EUR 521

Monkey ADAM10 ELISA Kit

EMKA0734 96Tests
EUR 521

Porcine ADAM10 ELISA Kit

EPA0734 96Tests
EUR 521

Rat ADAM10 ELISA Kit

ERA0734 96Tests
EUR 521

Rabbit ADAM10 ELISA Kit

ERTA0734 96Tests
EUR 521

Anserini ADAM10 ELISA Kit

EAA0734 96Tests
EUR 521

Goat ADAM10 ELISA Kit

EGTA0734 96Tests
EUR 521

Canine ADAM10 ELISA Kit

ECA0734 96Tests
EUR 521

Chicken ADAM10 ELISA Kit

ECKA0734 96Tests
EUR 521

Human ADAM10 ELISA Kit

EHA0734 96Tests
EUR 521

Bovine ADAM10 ELISA Kit

EBA0734 96Tests
EUR 521

ADAM10 Antibody

AF5294 200ul
EUR 304
Description: ADAM10 Antibody detects endogenous levels of total ADAM10.

ADAM10 Antibody

ABF5294 100 ug
EUR 438

ADAM10 antibody

70R-11689 100 ug
EUR 447
Description: Rabbit polyclonal ADAM10 antibody

ADAM10 antibody

70R-14074 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal ADAM10 antibody

ADAM10 Antibody

3201-100
EUR 338

ADAM10 antibody

10R-1576 100 ug
EUR 512
Description: Mouse monoclonal ADAM10 antibody

ADAM10 Antibody

1-CSB-PA069457
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

ADAM10 Antibody

1-CSB-PA909378
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

ADAM10 Antibody

1-CSB-PA001273LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

ADAM10 Antibody

1-CSB-PA000013
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

ADAM10 Antibody

24044-100ul 100ul
EUR 390

ADAM10 Antibody

36038-100ul 100ul
EUR 252

ADAM10 siRNA

20-abx906699
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

ADAM10 siRNA

20-abx906700
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

ADAM10 (Human)

GT15078 100 ug
EUR 526

ADAM10 (Mouse)

GT15079 100 ug
EUR 526

ADAM10 Antibody

49473-100ul 100ul
EUR 333

ADAM10 Antibody

49473-50ul 50ul
EUR 239

Rat Neural Stem Cell Kits

PC37123 1 X 10(6) *minimum order 6 vials*
EUR 957

MitoPT™ TMRE & TMRM Kits

KF17358 500 Test
EUR 479

MitoPT™ TMRE & TMRM Kits

KF17359 500 Test
EUR 479

HISTAR DETECTION SYSTEM Kits (OKSA11259)

OKSA11259 50 Tests
EUR 565
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11260)

OKSA11260 150 Tests
EUR 1335
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11261)

OKSA11261 500 Tests
EUR 2019
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

Guinea Pig ADAM10 ELISA Kit

EGA0734 96Tests
EUR 521

ADAM10 ELISA Kit (Human) (OKCD06783)

OKCD06783 96 Wells
EUR 753
Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 28pg/mL

ADAM10 ELISA Kit (Rat) (OKCD06785)

OKCD06785 96 Wells
EUR 818
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ELISA;Assay info: ;Sensitivity: < 27pg/mL

ADAM10 ELISA Kit (Human) (OKAN05106)

OKAN05106 96 Wells
EUR 792
Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 28 pg/mL

ADAM10 ELISA Kit (Rat) (OKAN05931)

OKAN05931 96 Wells
EUR 792
Description: Description of target: may act as a disintegrin-metalloprotease related to the reprolysin family [RGD, Feb 2006];Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 27 pg/mL

ADAM10 ELISA Kit (Rat) (OKEH06386)

OKEH06386 96 Wells
EUR 662
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.082 ng/mL

ADAM10 ELISA Kit (Mouse) (OKEH06827)

OKEH06827 96 Wells
EUR 779
Description: Description of target: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero with multiple defects of the developing central nervous system, somites, and cardiovascular system.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.081 ng/mL

ADAM10 ELISA Kit (Pig) (OKEH07468)

OKEH07468 96 Wells
EUR 1092
Description: Description of target: ;Species reactivity: Pig;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity:

ADAM10 Blocking Peptide

AF5294-BP 1mg
EUR 195

ADAM10 cloning plasmid

CSB-CL001273HU-10ug 10ug
EUR 376
Description: A cloning plasmid for the ADAM10 gene.

ADAM10 Polyclonal Antibody

ES1010-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ADAM10 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

ADAM10 Polyclonal Antibody

ES1010-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ADAM10 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

ADAM10 Rabbit pAb

A10438-100ul 100 ul
EUR 308

ADAM10 Rabbit pAb

A10438-200ul 200 ul
EUR 459

ADAM10 Rabbit pAb

A10438-20ul 20 ul
EUR 183

ADAM10 Rabbit pAb

A10438-50ul 50 ul
EUR 223

Anti-ADAM10 Antibody

A00566-1 100ug/vial
EUR 294

ADAM10 Polyclonal Antibody

A58010 100 µg
EUR 570.55
Description: reagents widely cited

ADAM10 Polyclonal Antibody

ABP50011-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of ADAM10 from Human. This ADAM10 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ADAM10 at AA range: 170-250

ADAM10 Polyclonal Antibody

ABP50011-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of ADAM10 from Human. This ADAM10 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ADAM10 at AA range: 170-250

ADAM10 Polyclonal Antibody

ABP50011-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of ADAM10 from Human. This ADAM10 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ADAM10 at AA range: 170-250

Human ADAM10 Protein

20-abx262305
  • EUR 328.00
  • EUR 5924.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

Anti-ADAM10 antibody

STJ112469 100 µl
EUR 277
Description: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.

Anti-ADAM10 antibody

STJ90014 200 µl
EUR 197
Description: Rabbit polyclonal to ADAM10.

ADAM10 Conjugated Antibody

C49473 100ul
EUR 397

ADAM10 (Human), CF

PR15036CF 20 ug
EUR 526

ADAM10 Conjugated Antibody

C36038 100ul
EUR 397

CellQuanti-Blue Cell Viability Assay Kits

CQBL-05K 5000
EUR 221
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 5000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-Blue Cell Viability Assay Kits

CQBL-10K 10000
EUR 340
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 10000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-MTT Cell Viability Assay Kits

CQMT-500 500
EUR 210
Description: Colorimetric (570nm) assay for cell viability, proliferation, cytotoxcity, HTS for anticancer agents. Kit size: 500 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11298)

OKSA11298 500 Tests
EUR 956
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11300)

OKSA11300 500 Tests
EUR 956
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

ADAM10 recombinant monoclonal antibody

A5298 100ul X 3
EUR 595
Description: A recombinant monoclonal antibody from rabbit against human ADAM10 for WB,ELISA

ADAM10 Antibody, HRP conjugated

1-CSB-PA001273LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ADAM10 Antibody, FITC conjugated

1-CSB-PA001273LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ADAM10 Antibody, Biotin conjugated

1-CSB-PA001273LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

ADAM10 Recombinant Protein (Human)

RP036424 100 ug Ask for price

ADAM10 Recombinant Protein (Mouse)

RP114161 100 ug Ask for price

Human ADAM10 shRNA Plasmid

20-abx950066
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse ADAM10 shRNA Plasmid

20-abx969028
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Adam10 High Sensitivity ELISA Kit (Mouse) (OKCD01334)

OKCD01334 96 Wells
EUR 844
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) at '687-Lys-|-Leu-688'. Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 3.7 pg/mL

ADAM10 ELISA Kit (Human) : 96 Wells (OKEH02627)

OKEH02627 96 Wells
EUR 740
Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.045 ng/mL

ADAM10 Chemi-Luminescent ELISA Kit (Rat) (OKCD03691)

OKCD03691 96 Wells
EUR 1066
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.078 ng/mL

ADAM10 ORF Vector (Human) (pORF)

ORF012142 1.0 ug DNA
EUR 354

Adam10 ORF Vector (Mouse) (pORF)

ORF038055 1.0 ug DNA
EUR 506

Anti-ADAM10 Rabbit Monoclonal Antibody

M00566 100ug/vial
EUR 397
Description: Rabbit Monoclonal ADAM10 Antibody. Validated in IP, WB and tested in Human, Mouse, Rat.

ADAM10 Polyclonal Antibody, Biotin Conjugated

A58011 100 µg
EUR 570.55
Description: Ask the seller for details

ADAM10 Polyclonal Antibody, FITC Conjugated

A58012 100 µg
EUR 570.55
Description: The best epigenetics products

ADAM10 Polyclonal Antibody, HRP Conjugated

A58013 100 µg
EUR 570.55
Description: kits suitable for this type of research

ADAM10, Human Recombinant Protein, E.Coli

CG1002-002 2 ug
EUR 226

ADAM10, Human Recombinant Protein, E.Coli

CG1002-010 10 ug
EUR 300

Human ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

20-abx150542
  • EUR 6642.00
  • EUR 3542.00
  • EUR 825.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

20-abx153594
  • EUR 6642.00
  • EUR 3542.00
  • EUR 825.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx575998-96tests 96 tests
EUR 707

Rat ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx576388-96tests 96 tests
EUR 668

Cow ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx513940-96tests 96 tests
EUR 911

Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx513942-96tests 96 tests
EUR 668

Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

20-abx258362
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Pig ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx361975-96tests 96 tests
EUR 825

Rabbit ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx363169-96tests 96 tests
EUR 825

Monkey ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx360194-96tests 96 tests
EUR 825

Chicken ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx356774-96tests 96 tests
EUR 825

Rat ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

20-abx155141
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx250281-96tests 96 tests
EUR 707

Adam10 sgRNA CRISPR Lentivector set (Mouse)

K4401401 3 x 1.0 ug
EUR 339

ADAM10 sgRNA CRISPR Lentivector set (Human)

K0041801 3 x 1.0 ug
EUR 339

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

abx147972-100ug 100 ug
EUR 439

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

20-abx125489
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

abx038455-100ug 100 ug
EUR 391

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

abx412491-01mg 0.1 mg
EUR 509

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

20-abx301124
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

20-abx323613
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

20-abx241176
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

20-abx214006
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Mast/stem Cell Growth Factor Receptor Kit (Kits) Antibody

20-abx116620
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Rabbit ANTI 14-3-3 ISOFORM PANEL Kits (OKSA11258)

OKSA11258 1 u
EUR 1236
Description: Description of target: RABBIT ANTI 14-3-3 ISOFORM PANEL;Species reactivity: Sheep;Application: ELISA, IHC-FFPE, WB;Assay info: ;Sensitivity:

Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit

CN-03469H1 96T
EUR 452

Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit

CN-03469H2 48T
EUR 299

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Hu-10x96wellstestplate 10x96-wells test plate
EUR 4273.35
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human A Disintegrin And Metalloprotease 10 (ADAM10) in serum, plasma, tissue homogenates and other biological fluids.

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Hu-1x48wellstestplate 1x48-wells test plate
EUR 439.57
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human A Disintegrin And Metalloprotease 10 (ADAM10) in serum, plasma, tissue homogenates and other biological fluids.

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Hu-1x96wellstestplate 1x96-wells test plate
EUR 585.1
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human A Disintegrin And Metalloprotease 10 (ADAM10) in serum, plasma, tissue homogenates and other biological fluids.

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Hu-5x96wellstestplate 5x96-wells test plate
EUR 2332.95
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human A Disintegrin And Metalloprotease 10 (ADAM10) in serum, plasma, tissue homogenates and other biological fluids.

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

4-SEA766Hu
  • EUR 4324.00
  • EUR 2283.00
  • EUR 586.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human A Disintegrin And Metalloprotease 10 (ADAM10) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Mu-10x96wellstestplate 10x96-wells test plate
EUR 4391.16
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse A Disintegrin And Metalloprotease 10 (ADAM10) in tissue homogenates, cell lysates and other biological fluids.

Mouse A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Mu-1x48wellstestplate 1x48-wells test plate
EUR 449.27
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse A Disintegrin And Metalloprotease 10 (ADAM10) in tissue homogenates, cell lysates and other biological fluids.

Mouse A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Mu-1x96wellstestplate 1x96-wells test plate
EUR 598.96
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse A Disintegrin And Metalloprotease 10 (ADAM10) in tissue homogenates, cell lysates and other biological fluids.

Mouse A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Mu-5x96wellstestplate 5x96-wells test plate
EUR 2395.32
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse A Disintegrin And Metalloprotease 10 (ADAM10) in tissue homogenates, cell lysates and other biological fluids.

Mouse A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

4-SEA766Mu
  • EUR 4442.00
  • EUR 2346.00
  • EUR 599.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse A Disintegrin And Metalloprotease 10 (ADAM10) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Ra-10x96wellstestplate 10x96-wells test plate
EUR 4626.78
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat A Disintegrin And Metalloprotease 10 (ADAM10) in serum, plasma, tissue homogenates and other biological fluids.

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Ra-1x48wellstestplate 1x48-wells test plate
EUR 468.68
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat A Disintegrin And Metalloprotease 10 (ADAM10) in serum, plasma, tissue homogenates and other biological fluids.

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Ra-1x96wellstestplate 1x96-wells test plate
EUR 626.68
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat A Disintegrin And Metalloprotease 10 (ADAM10) in serum, plasma, tissue homogenates and other biological fluids.

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

SEA766Ra-5x96wellstestplate 5x96-wells test plate
EUR 2520.06
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat A Disintegrin And Metalloprotease 10 (ADAM10) in serum, plasma, tissue homogenates and other biological fluids.

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

4-SEA766Ra
  • EUR 4677.00
  • EUR 2471.00
  • EUR 627.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat A Disintegrin And Metalloprotease 10 (ADAM10) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human A Disintegrin And Metalloprotease 10, ADAM10 ELISA Kit

CSB-E11172h-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human A Disintegrin And Metalloprotease 10, ADAM10 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human A Disintegrin And Metalloprotease 10, ADAM10 ELISA Kit

1-CSB-E11172h
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human A Disintegrin And Metalloprotease 10, ADAM10 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human A Disintegrin And Metalloprotease 10(ADAM10)ELISA Kit

GA-E0785HM-48T 48T
EUR 289

Human A Disintegrin And Metalloprotease 10(ADAM10)ELISA Kit

GA-E0785HM-96T 96T
EUR 466

Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit

201-12-0769 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Mouse A Disintegrin And Metalloprotease 10 ELISA Kit (ADAM10)

RK02570 96 Tests
EUR 521

Rat A Disintegrin And Metalloprotease 10 ELISA Kit (ADAM10)

RK03467 96 Tests
EUR 521

Human A Disintegrin And Metalloprotease 10 ELISA Kit (ADAM10)

RK00811 96 Tests
EUR 521

Human A Disintegrin And Metalloprotease 10(ADAM10)ELISA Kit

QY-E02774 96T
EUR 361

Rat A Disintegrin And Metalloprotease 10(ADAM10)ELISA kit

QY-E10418 96T
EUR 361

Mouse A Disintegrin And Metalloprotease 10(ADAM10)ELISA kit

QY-E20025 96T
EUR 361

Human Anti-Chikungunya virus (CHIKV) IgM capture ELISA kits, 96 tests, qualitative

910-705-CHM 1 Kit
EUR 834

ADAM10 3'UTR Luciferase Stable Cell Line

TU000297 1.0 ml
EUR 1521

Adam10 3'UTR Luciferase Stable Cell Line

TU101352 1.0 ml Ask for price

Adam10 3'UTR GFP Stable Cell Line

TU151352 1.0 ml Ask for price
We present the event of biochemical assays in normal buffers for wild-type isocitrate dehydrogenase 1 (IDH1), diacylglycerol kinase zeta (DGKζ), and p300 histone acetyltransferase (P300) to show the suitability of this technique for a broad vary of high-throughput lead discovery assays. A proof-of-concept pilot display of ∼3k compounds can be proven for IDH1 and in comparison with a beforehand reported fluorescence-based assay. We had been capable of get hold of dependable knowledge at a velocity amenable for high-throughput screening of large-scale compound libraries.

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