Reconstitution and biochemical assays of an active human histone pre-mRNA 3′-end processing machinery

Reconstitution and biochemical assays of an active human histone pre-mRNA 3'-end processing machinery
In animal cells, replication-dependent histone pre-mRNAs are processed on the 3′-end by an endonucleolytic cleavage carried out by the U7 snRNP, a machinery that comprises the U7 snRNA and many protein subunits. Studies on the composition of this machinery and understanding of its position in 3′-end processing had been enormously facilitated by the event of an in vitro system using nuclear extracts from mammalian cells 35 years in the past and later from Drosophila cells.
Most lately, recombinant expression and purification of the elements of the machinery have enabled the total reconstitution of an active machinery and its complicated with a mannequin pre-mRNA substrate, utilizing 13 proteins and 2 RNAs, and the willpower of the construction of this active machinery. This chapter presents protocols for making ready nuclear extracts containing endogenous processing machinery, for assembling semi-recombinant and totally reconstituted machineries, and for histone pre-mRNA 3′-end processing assays with these samples.

Optimization of biochemical sulfide potential (BSP) assay for anaerobic biodegradability evaluation

The anaerobic biodegradability evaluation (biodegradation extent and kinetics) of natural wastes is vital for optimum design and evaluating therapy efficiencies for anaerobic therapy applied sciences. The biochemical sulfide potential (BSP) assay has beforehand demonstrated the benefits of its time effectivity and measurement accuracy for biologically assessing substrate degradability, whereas its software is proscribed by undefined operational parameters. In this research, the BSP assay was additional optimized via a scientific investigation of a vital parameter, inoculum-to-substrate ratio (ISR), and the relevant kinetic mannequin to unravel the potential use of BSP assays for anaerobic waste therapy.
Under two collection of experimental eventualities, the widespread ISR ranges of 0.5-4.0 (based mostly on the standard BMP assay) and excessive ISRs (as little as 0.1) had been studied, by which the benefit of a BSP assay on excessive ISRs was highlighted. Meanwhile, the underlying trigger and mechanism for biodegradability discrepancies beneath totally different ISRs (0.1-6.0) had been additional investigated.
Reconstitution and biochemical assays of an active human histone pre-mRNA 3'-end processing machinery
The extracellular polymeric substance (EPS) characterization of residual organics and the two-substrate first-order hydrolysis mannequin analyses revealed that the hydrolysis course of of slowly-biodegradable organics fraction was hindered beneath improper ISR circumstances. Furthermore, the Cone mannequin was evaluated as extra acceptable for biodegradation kinetics evaluation in BSP assays among the many 5 widespread kinetic fashions (i.e., Exponential, Fitzhugh, Cone, Transference, and modified Gompertz fashions). Overall, the outcomes present basic steerage on designing constant BSP assays and put a step ahead in standardizing the BSP assay for anaerobic biodegradability assessments.

Assessment of structural protein expression by FTIR and biochemical assays as biomarkers of metabolites response in gastric and colon most cancers

Colon and gastric cancers are the widespread benign varieties of cancers that are synchronous and metachronous neoplasms. In phrases of the development and progress of the illness, metabolic processes and differentiation in protein buildings have an vital position in for therapy of the illness. In this research we proposed to analyze the metabolic course of and the differentiation of protein secondary construction amongst colon and gastric most cancers in addition to wholesome controls utilizing biochemistry and Fourier Transform InfraRed spectroscopy (FTIR) strategies. For this goal, we measured blood serum of 133 sufferers, which had been performed upon oncology division (45 colon most cancers, 45 gastric most cancers and 43 management people).
The obtained spectroscopic outcomes and biochemical assays confirmed vital discount within the quantity of useful teams in most cancers teams opposite with whole protein measurements and construction of protein variations between colon and gastric cancers. Differentiations had been seen in serum ranges of CEA, CA-125, CA-15-3, CA-19-9 AFP (Alpha fetoprotein) of gastric and colon most cancers sufferers in addition to in amide III and secondly described amide I areas. Our findings recommend that amide I bonds in colon most cancers cells will be useful in prognosis of colon most cancers.
Indeed, our outcomes confirmed that metabolic processes had been greater in gastric most cancers group than in colon most cancers. Hence, FTIR spectroscopy and curve-fitting evaluation of amide I profile will be efficiently utilized as instruments for figuring out quantitative and qualitative adjustments of proteins in human cancerous blood serum. However, what is essential, in PCA evaluation we see, that the scatter plot of PC1 (variability 80%) and PC2 (variability 15%) present that the information associated to the management and two most cancers teams are clustered along with totally different magnitudes and instructions.

High-Throughput Label-Free Biochemical Assays Using Infrared Matrix-Assisted Desorption Electrospray Ionization Mass Spectrometry

Mass spectrometry (MS) can present excessive sensitivity and specificity for biochemical assays with out the requirement of labels, eliminating the danger of assay interference. However, its use had been restricted to lower-throughput assays because of the want for chromatography to beat ion suppression from the pattern matrix.
Direct evaluation with out chromatography has the potential for top throughput if sensitivity is ample regardless of the presence of a matrix. Here, we report and show a novel direct evaluation high-throughput MS system based mostly on infrared matrix-assisted desorption electrospray ionization (IR-MALDESI) that has a possible acquisition price of 33 spectra/s.

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Hu-48Tests Reddot Biotech 48 Tests 500 EUR

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Hu-96Tests Reddot Biotech 96 Tests 692 EUR

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Ra-48Tests Reddot Biotech 48 Tests 534 EUR

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

RDR-ADAM10-Ra-96Tests Reddot Biotech 96 Tests 742 EUR

Adam10/ Rat Adam10 ELISA Kit

ELI-02616r Lifescience Market 96 Tests 886 EUR

Anti-ADAM10/ADAM10 Antibody

PA1535 BosterBio 100ug/vial 294 EUR

ADAM10

PR27192 Neuromics 2 ug 191 EUR

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 Alpha Diagnostics 1 202 EUR

ADAM10 Antibody

1-CSB-PA001273LA01HU Cusabio
  • 317.00 EUR
  • 335.00 EUR
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

ADAM10 Antibody

1-CSB-PA000013 Cusabio
  • 222.00 EUR
  • 195.00 EUR
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

ADAM10 Antibody

1-CSB-PA069457 Cusabio
  • 317.00 EUR
  • 244.00 EUR
  • 100ul
  • 50ul
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

ADAM10 Antibody

1-CSB-PA909378 Cusabio
  • 317.00 EUR
  • 244.00 EUR
  • 100ul
  • 50ul
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

ADAM10 (Human)

GT15078 Neuromics 100 ug 526 EUR

ADAM10 (Mouse)

GT15079 Neuromics 100 ug 526 EUR

ADAM10 Antibody

24044-100ul SAB 100ul 390 EUR

ADAM10 Antibody

36038-100ul SAB 100ul 252 EUR

ADAM10 siRNA

20-abx906699 Abbexa
  • 551.00 EUR
  • 732.00 EUR
  • 15 nmol
  • 30 nmol

ADAM10 siRNA

20-abx906700 Abbexa
  • 551.00 EUR
  • 732.00 EUR
  • 15 nmol
  • 30 nmol

ADAM10 Antibody

49473-100ul SAB 100ul 333 EUR

ADAM10 Antibody

49473-50ul SAB 50ul 239 EUR

ADAM10 Antibody

AF5294 Affbiotech 200ul 304 EUR
Description: ADAM10 Antibody detects endogenous levels of total ADAM10.

ADAM10 Antibody

ABF5294 Lifescience Market 100 ug 438 EUR

ADAM10 Antibody

2051-002mg ProSci 0.02 mg 171.82 EUR
Description: ADAM10 Antibody: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

ADAM10 Antibody

2051-01mg ProSci 0.1 mg 436.42 EUR
Description: ADAM10 Antibody: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

ADAM10 Peptide

2051P ProSci 0.05 mg 164.75 EUR
Description: (CT) ADAM10 peptide

ADAM10 antibody

10R-1576 Fitzgerald 100 ug 512 EUR
Description: Mouse monoclonal ADAM10 antibody

ADAM10 antibody

70R-11689 Fitzgerald 100 ug 447 EUR
Description: Rabbit polyclonal ADAM10 antibody

ADAM10 antibody

70R-14074 Fitzgerald 100 ug 322 EUR
Description: Affinity purified Rabbit polyclonal ADAM10 antibody

ADAM10 Antibody

3201-100 Biovision 338 EUR

Sheep ADAM10 ELISA Kit

ESA0734 Abclonal 96Tests 521 EUR

Mouse ADAM10 ELISA Kit

EMA0734 Abclonal 96Tests 521 EUR

Anserini ADAM10 ELISA Kit

EAA0734 Abclonal 96Tests 521 EUR

Rabbit ADAM10 ELISA Kit

ERTA0734 Abclonal 96Tests 521 EUR

Monkey ADAM10 ELISA Kit

EMKA0734 Abclonal 96Tests 521 EUR

Porcine ADAM10 ELISA Kit

EPA0734 Abclonal 96Tests 521 EUR

Rat ADAM10 ELISA Kit

ERA0734 Abclonal 96Tests 521 EUR

Human ADAM10 ELISA Kit

EHA0734 Abclonal 96Tests 521 EUR

Canine ADAM10 ELISA Kit

ECA0734 Abclonal 96Tests 521 EUR

Chicken ADAM10 ELISA Kit

ECKA0734 Abclonal 96Tests 521 EUR

Goat ADAM10 ELISA Kit

EGTA0734 Abclonal 96Tests 521 EUR

Bovine ADAM10 ELISA Kit

EBA0734 Abclonal 96Tests 521 EUR

Human ADAM10 ELISA Kit

ELA-E0766h Lifescience Market 96 Tests 824 EUR

Mouse Adam10 ELISA KIT

ELI-02614m Lifescience Market 96 Tests 865 EUR

Bovine ADAM10 ELISA KIT

ELI-02617b Lifescience Market 96 Tests 928 EUR

Human ADAM10 ELISA KIT

ELI-02618h Lifescience Market 96 Tests 824 EUR

ADAM10 ELISA KIT|Human

EF001085 Lifescience Market 96 Tests 689 EUR

Guinea Pig ADAM10 ELISA Kit

EGA0734 Abclonal 96Tests 521 EUR

ADAM10 ELISA Kit (Human) (OKCD06783)

OKCD06783 Aviva Systems Biology 96 Wells 753 EUR
Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 28pg/mL

ADAM10 ELISA Kit (Rat) (OKCD06785)

OKCD06785 Aviva Systems Biology 96 Wells 818 EUR
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ELISA;Assay info: ;Sensitivity: < 27pg/mL

ADAM10 ELISA Kit (Human) (OKAN05106)

OKAN05106 Aviva Systems Biology 96 Wells 792 EUR
Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 28 pg/mL

ADAM10 ELISA Kit (Rat) (OKAN05931)

OKAN05931 Aviva Systems Biology 96 Wells 792 EUR
Description: Description of target: may act as a disintegrin-metalloprotease related to the reprolysin family [RGD, Feb 2006];Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 27 pg/mL

ADAM10 ELISA Kit (Rat) (OKEH06386)

OKEH06386 Aviva Systems Biology 96 Wells 662 EUR
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.082 ng/mL

ADAM10 ELISA Kit (Mouse) (OKEH06827)

OKEH06827 Aviva Systems Biology 96 Wells 779 EUR
Description: Description of target: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero with multiple defects of the developing central nervous system, somites, and cardiovascular system.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.081 ng/mL

ADAM10 ELISA Kit (Pig) (OKEH07468)

OKEH07468 Aviva Systems Biology 96 Wells 1092 EUR
Description: Description of target: ;Species reactivity: Pig;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity:

ADAM10 cloning plasmid

CSB-CL001273HU-10ug Cusabio 10ug 376 EUR
Description: A cloning plasmid for the ADAM10 gene.

ADAM10 Polyclonal Antibody

ES1010-100ul ELK Biotech 100ul 279 EUR
Description: A Rabbit Polyclonal antibody against ADAM10 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

ADAM10 Polyclonal Antibody

ES1010-50ul ELK Biotech 50ul 207 EUR
Description: A Rabbit Polyclonal antibody against ADAM10 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Anti-ADAM10 antibody

STJ112469 St John's Laboratory 100 µl 277 EUR
Description: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.

ADAM10 (Human), CF

PR15036CF Neuromics 20 ug 526 EUR

Anti-ADAM10 Antibody

A00566-1 BosterBio 100ug/vial 294 EUR

ADAM10 Polyclonal Antibody

ABP50011-003ml Abbkine 0.03ml 158 EUR
Description: A polyclonal antibody for detection of ADAM10 from Human. This ADAM10 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ADAM10 at AA range: 170-250

ADAM10 Polyclonal Antibody

ABP50011-01ml Abbkine 0.1ml 289 EUR
Description: A polyclonal antibody for detection of ADAM10 from Human. This ADAM10 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ADAM10 at AA range: 170-250

ADAM10 Polyclonal Antibody

ABP50011-02ml Abbkine 0.2ml 414 EUR
Description: A polyclonal antibody for detection of ADAM10 from Human. This ADAM10 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ADAM10 at AA range: 170-250

ADAM10 Polyclonal Antibody

A58010 EpiGentek 100 µg 570.55 EUR
Description: reagents widely cited

ADAM10 Rabbit pAb

A10438-100ul Abclonal 100 ul 308 EUR

ADAM10 Rabbit pAb

A10438-200ul Abclonal 200 ul 459 EUR

ADAM10 Rabbit pAb

A10438-20ul Abclonal 20 ul 183 EUR

ADAM10 Rabbit pAb

A10438-50ul Abclonal 50 ul 223 EUR

Human ADAM10 Protein

20-abx262305 Abbexa
  • 328.00 EUR
  • 5924.00 EUR
  • 230.00 EUR
  • 10 ug
  • 1 mg
  • 2 µg

Anti-ADAM10 antibody

STJ90014 St John's Laboratory 200 µl 197 EUR
Description: Rabbit polyclonal to ADAM10.

ADAM10 Conjugated Antibody

C36038 SAB 100ul 397 EUR

ADAM10 Conjugated Antibody

C49473 SAB 100ul 397 EUR

ADAM10 Blocking Peptide

AF5294-BP Affbiotech 1mg 195 EUR

T&A Cloning Kits

FYC001-20P Yeastern Biotech 1 vial Ask for price

Necrosis vs Apoptosis Kits

KF17371 Neuromics 50-100 Tests 435 EUR

Necrosis vs Apoptosis Kits

KF17372 Neuromics 100-200 Tests 768 EUR

Basic Cytotoxicity Test Kits

CT17001 Neuromics 125 Tests 409 EUR

Basic Cytotoxicity Test Kits

CT17002 Neuromics 250 Tests 650 EUR

Total Cytotoxicity Test Kits

CT17003 Neuromics 125 Tests 513 EUR

Total Cytotoxicity Test Kits

CT17004 Neuromics 250 Tests 813 EUR

Pipette Stand Starter Kits

P7700-S1 Benchmark Scientific 1 PC 507.83 EUR

Pipette Stand Starter Kits

P7700-S2 Benchmark Scientific 1 PC 526.1 EUR

ADAM10 Antibody, HRP conjugated

1-CSB-PA001273LB01HU Cusabio
  • 317.00 EUR
  • 335.00 EUR
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ADAM10 Antibody, FITC conjugated

1-CSB-PA001273LC01HU Cusabio
  • 317.00 EUR
  • 335.00 EUR
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ADAM10 Antibody, Biotin conjugated

1-CSB-PA001273LD01HU Cusabio
  • 317.00 EUR
  • 335.00 EUR
  • 100ug
  • 50ug
Description: A polyclonal antibody against ADAM10. Recognizes ADAM10 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Mouse ADAM10 shRNA Plasmid

20-abx969028 Abbexa
  • 801.00 EUR
  • 1121.00 EUR
  • 150 µg
  • 300 µg

Human ADAM10 shRNA Plasmid

20-abx950066 Abbexa
  • 801.00 EUR
  • 1121.00 EUR
  • 150 µg
  • 300 µg

ADAM10 recombinant monoclonal antibody

A5298 Bimake 100ul X 3 595 EUR
Description: A recombinant monoclonal antibody from rabbit against human ADAM10 for WB,ELISA

ADAM10 Recombinant Protein (Mouse)

RP114161 ABM 100 ug Ask for price

ADAM10 Recombinant Protein (Human)

RP036424 ABM 100 ug Ask for price

Rat Neural Stem Cell Kits

PC37123 Neuromics 1 X 10(6) *minimum order 6 vials* 957 EUR

MitoPT™ TMRE & TMRM Kits

KF17358 Neuromics 500 Test 479 EUR

MitoPT™ TMRE & TMRM Kits

KF17359 Neuromics 500 Test 479 EUR

HISTAR DETECTION SYSTEM Kits (OKSA11259)

OKSA11259 Aviva Systems Biology 50 Tests 565 EUR
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11260)

OKSA11260 Aviva Systems Biology 150 Tests 1335 EUR
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11261)

OKSA11261 Aviva Systems Biology 500 Tests 2019 EUR
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

ADAM10 ELISA Kit (Human) : 96 Wells (OKEH02627)

OKEH02627 Aviva Systems Biology 96 Wells 740 EUR
Description: Description of target: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.045 ng/mL

ADAM10 Chemi-Luminescent ELISA Kit (Rat) (OKCD03691)

OKCD03691 Aviva Systems Biology 96 Wells 1066 EUR
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.078 ng/mL

Adam10 High Sensitivity ELISA Kit (Mouse) (OKCD01334)

OKCD01334 Aviva Systems Biology 96 Wells 844 EUR
Description: Description of target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) at '687-Lys-|-Leu-688'. Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 3.7 pg/mL

Nori® Porcine 3-Plex ELISA Kits-Interferons

GR224009 Genorise Scientific 96-well 503 EUR

Nori® Porcine 4-Plex ELISA Kits-Immunoglobulins

GR224017 Genorise Scientific 96-well 545 EUR

Nori® Porcine 6-Plex ELISA Kits-BMP

GR224025 Genorise Scientific 96-well 629 EUR

Nori® Porcine 6-Plex ELISA Kits-CCL

GR224026 Genorise Scientific 96-well 629 EUR

Nori® Porcine 6-Plex ELISA Kits-MMP

GR224027 Genorise Scientific 96-well 629 EUR

Nori® Porcine 6-Plex ELISA Kits-TLR

GR224028 Genorise Scientific 96-well 629 EUR

Nori® Porcine 6-Plex ELISA Kits-Hormones

GR224029 Genorise Scientific 96-well 629 EUR

Nori® Porcine 6-Plex ELISA Kits-SAA

GR224030 Genorise Scientific 96-well 629 EUR

Nori® Porcine 6-Plex ELISA Kits-S100

GR224031 Genorise Scientific 96-well 629 EUR

Nori® Porcine 6-Plex ELISA Kits-CXCL

GR224032 Genorise Scientific 96-well 629 EUR

Nori® Equine 3-Plex ELISA Kits-Interferons

GR225009 Genorise Scientific 96-well 503 EUR

Nori® Equine 4-Plex ELISA Kits-Immunoglobulins

GR225017 Genorise Scientific 96-well 545 EUR

Nori® Equine 6-Plex ELISA Kits-BMP

GR225025 Genorise Scientific 96-well 629 EUR

Nori® Equine 6-Plex ELISA Kits-CCL

GR225026 Genorise Scientific 96-well 629 EUR

Nori® Equine 6-Plex ELISA Kits-MMP

GR225027 Genorise Scientific 96-well 629 EUR

Nori® Equine 6-Plex ELISA Kits-TLR

GR225028 Genorise Scientific 96-well 629 EUR

Nori® Equine 6-Plex ELISA Kits-Hormones

GR225029 Genorise Scientific 96-well 629 EUR

Nori® Equine 6-Plex ELISA Kits-SAA

GR225030 Genorise Scientific 96-well 629 EUR

Nori® Equine 6-Plex ELISA Kits-S100

GR225031 Genorise Scientific 96-well 629 EUR

Nori® Equine 6-Plex ELISA Kits-CXCL

GR225032 Genorise Scientific 96-well 629 EUR

Nori® Canine 3-Plex ELISA Kits-Interferons

GR226009 Genorise Scientific 96-well 503 EUR

Nori® Canine 4-Plex ELISA Kits-Immunoglobulins

GR226017 Genorise Scientific 96-well 545 EUR

Nori® Canine 6-Plex ELISA Kits-BMP

GR226025 Genorise Scientific 96-well 629 EUR

Nori® Canine 6-Plex ELISA Kits-CCL

GR226026 Genorise Scientific 96-well 629 EUR

Nori® Canine 6-Plex ELISA Kits-MMP

GR226027 Genorise Scientific 96-well 629 EUR

Nori® Canine 6-Plex ELISA Kits-TLR

GR226028 Genorise Scientific 96-well 629 EUR

Nori® Canine 6-Plex ELISA Kits-Hormones

GR226029 Genorise Scientific 96-well 629 EUR

Nori® Canine 6-Plex ELISA Kits-SAA

GR226030 Genorise Scientific 96-well 629 EUR

Nori® Canine 6-Plex ELISA Kits-S100

GR226031 Genorise Scientific 96-well 629 EUR

Nori® Canine 6-Plex ELISA Kits-CXCL

GR226032 Genorise Scientific 96-well 629 EUR

Nori® Bovine 3-Plex ELISA Kits-Interferons

GR228009 Genorise Scientific 96-well 503 EUR

Nori® Bovine 4-Plex ELISA Kits-Immunoglobulins

GR228017 Genorise Scientific 96-well 545 EUR

Nori® Bovine 6-Plex ELISA Kits-BMP

GR228025 Genorise Scientific 96-well 629 EUR

Nori® Bovine 6-Plex ELISA Kits-CCL

GR228026 Genorise Scientific 96-well 629 EUR

Nori® Bovine 6-Plex ELISA Kits-MMP

GR228027 Genorise Scientific 96-well 629 EUR

Nori® Bovine 6-Plex ELISA Kits-TLR

GR228028 Genorise Scientific 96-well 629 EUR

Nori® Bovine 6-Plex ELISA Kits-Hormones

GR228029 Genorise Scientific 96-well 629 EUR

Nori® Bovine 6-Plex ELISA Kits-SAA

GR228030 Genorise Scientific 96-well 629 EUR

Nori® Bovine 6-Plex ELISA Kits-S100

GR228031 Genorise Scientific 96-well 629 EUR

Nori® Bovine 6-Plex ELISA Kits-CXCL

GR228032 Genorise Scientific 96-well 629 EUR

Anti-ADAM10 Rabbit Monoclonal Antibody

M00566 BosterBio 100ug/vial 397 EUR
Description: Rabbit Monoclonal ADAM10 Antibody. Validated in IP, WB and tested in Human, Mouse, Rat.

ADAM10, Human Recombinant Protein, E.Coli

CG1002-002 GenDepot 2 ug 226 EUR

ADAM10, Human Recombinant Protein, E.Coli

CG1002-010 GenDepot 10 ug 300 EUR

ADAM10 Polyclonal Antibody, Biotin Conjugated

A58011 EpiGentek 100 µg 570.55 EUR
Description: Ask the seller for details

ADAM10 Polyclonal Antibody, FITC Conjugated

A58012 EpiGentek 100 µg 570.55 EUR
Description: The best epigenetics products

ADAM10 Polyclonal Antibody, HRP Conjugated

A58013 EpiGentek 100 µg 570.55 EUR
Description: kits suitable for this type of research

Adam10 ORF Vector (Mouse) (pORF)

ORF038055 ABM 1.0 ug DNA 506 EUR

ADAM10 ORF Vector (Human) (pORF)

ORF012142 ABM 1.0 ug DNA 354 EUR

MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11298)

OKSA11298 Aviva Systems Biology 500 Tests 956 EUR
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11300)

OKSA11300 Aviva Systems Biology 500 Tests 956 EUR
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

CellQuanti-Blue Cell Viability Assay Kits

CQBL-05K BioAssay Systems 5000 221 EUR
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 5000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-Blue Cell Viability Assay Kits

CQBL-10K BioAssay Systems 10000 340 EUR
Description: Homogeneous assay for cell viability, proliferation, cytotoxcity, high-throµghput screening for anticancer agents. Fluorimetric method (530nm/590nm). Kit size: 10000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

CellQuanti-MTT Cell Viability Assay Kits

CQMT-500 BioAssay Systems 500 210 EUR
Description: Colorimetric (570nm) assay for cell viability, proliferation, cytotoxcity, HTS for anticancer agents. Kit size: 500 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

Human ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx575998-96tests Abbexa 96 tests 707 EUR

Monkey ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx360194-96tests Abbexa 96 tests 825 EUR

Pig ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx361975-96tests Abbexa 96 tests 825 EUR

Rabbit ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx363169-96tests Abbexa 96 tests 825 EUR

Chicken ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx356774-96tests Abbexa 96 tests 825 EUR

Cow ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx513940-96tests Abbexa 96 tests 911 EUR

Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx513942-96tests Abbexa 96 tests 668 EUR

Rat ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx576388-96tests Abbexa 96 tests 668 EUR

Human ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

20-abx150542 Abbexa
  • 6642.00 EUR
  • 3542.00 EUR
  • 825.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

abx250281-96tests Abbexa 96 tests 707 EUR

Rat ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

20-abx155141 Abbexa
  • 7237.00 EUR
  • 3855.00 EUR
  • 895.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

20-abx153594 Abbexa
  • 6642.00 EUR
  • 3542.00 EUR
  • 825.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit

20-abx258362 Abbexa
  • 7378.00 EUR
  • 3933.00 EUR
  • 911.00 EUR
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Nori® Porcine 6-Plex ELISA Kits-Growth Factors

GR224024 Genorise Scientific 96-well 629 EUR
We present the event of biochemical assays in normal buffers for wild-type isocitrate dehydrogenase 1 (IDH1), diacylglycerol kinase zeta (DGKζ), and p300 histone acetyltransferase (P300) to show the suitability of this technique for a broad vary of high-throughput lead discovery assays. A proof-of-concept pilot display of ∼3k compounds can be proven for IDH1 and in comparison with a beforehand reported fluorescence-based assay. We had been capable of get hold of dependable knowledge at a velocity amenable for high-throughput screening of large-scale compound libraries.

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