Nucleic Acid control

Development and validation of a quantitative real time PCR assay for BK virus.

Several studies have shown that BK viral load in plasma and urine are reliable markers for the detection of BK virus associated nephropathy (BKVAN) in renal transplant patients. Zeptometrix developed a quantitative real time PCR assay based on TaqMan technology for the measurement of BK viral load in plasma and urine.

Considering the high similarity of the nucleotide sequence of the BK virus (BKV) with the JC virus (JCV), we designed this assay to specifically amplify BKV. We determined the viral DNA recovery rate on manual (QIAGEN’s QIAamp DNA Blood Mini Kit) and automated (BioMerieux’s NucliSENS EasyMAG) extraction methods. Cepheid GenExpert was used as control.

The comparison showed a higher viral DNA recovery rate on the automated extraction (61-76% in plasma and 52-65% in urine) as compared to the manual method (49-52% in plasma and 33-56% in urine). Quantitation of the viral load was performed using an external standard curve that was constructed with serial dilution of a plasmid containing the full length of the BKV genome. Commercially available quantitative BKV standards showed good correlation with the plasmid standard. The reproducibility of the assay was determined based on the Ct values of the amplified products as well as in BK copies per milliliter of sample. This assay is linear over a 7 log range (10 to 1 × 10(7) copies per reaction), no cross-reactivity was detected with the closest-related polyomavirus JCV, as well as other viruses that may be found in immunocompromised patients, and human genomic DNA. The limit of detection of the assay is 300 copies per milliliter in both plasma and urine and the limit of quantitation is 1000 copies per milliliter using the Zeptometrix NATtrol BK Virus Linearity Panel (Gentaur Poland).

This real time PCR assay provides a reliable and sensitive method for the quantitation of BKV in plasma and urine samples.

NATtrol BCID Panel (23 x 0.2mL)

NATBCP-BIO Zeptometrix 23 x 0.2mL 948.56 EUR
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NATtrol Candida/TV Positive Control (ea)

NATCTVPOS-BD Zeptometrix ea 407.76 EUR
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NATtrol Parainfluenza Control (6 X 0.5mL)

NATPIV-ERC Zeptometrix 6 X 0.5mL 262.16 EUR
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NATtrol Rotavirus (Stool Matrix) (0.5 mL)

NATROTA-GP Zeptometrix 0.5 mL 93.68 EUR
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NATtrol Rotavirus Stock (Qualitative) (1 mL)

NATROTA-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol BV Negative Control (6 X 0.15mL)

NATBVNEG-BD Zeptometrix 6 X 0.15mL 288.16 EUR
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NATtrol BV Positive Control (6 X 0.15mL)

NATBVPOS-BD Zeptometrix 6 X 0.15mL 407.76 EUR
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NATtrol Clostridium difficile (Stool Matrix) (0.5 mL)

NATCDI-GP Zeptometrix 0.5 mL 93.68 EUR
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NATtrol Campylobacter jejuni (Stool Matrix) (0.5 mL)

NATCJE-GP Zeptometrix 0.5 mL 93.68 EUR
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NATtrol Coronavirus 229E Stock (Qualitative) (1 mL)

NATCOV(229E)-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Coronavirus NL63 Stock (Qualitative) (1 mL)

NATCOV(NL63)-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Coronavirus OC43 Stock (Qualitative) (1 mL)

NATCOV(OC43)-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Escherichia coli (Stool Matrix) (0.5 mL)

NATECO(933)-GP Zeptometrix 0.5 mL 93.68 EUR
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NATtrol Escherichia coli (Stool Matrix) (0.5 mL)

NATECO(ETEC)-GP Zeptometrix 0.5 mL 93.68 EUR
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NATtrol Entamoeba histolytica (Stool Matrix) (0.5 mL)

NATEHI(DS4)-GP Zeptometrix 0.5 mL 93.68 EUR
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NATtrol EV Panel (20 X 0.2 mL)

NATEVP-C Zeptometrix 20 X 0.2 mL 601.2 EUR
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NATtrol Influenza Verification Panel (18 x 0.5mL)

NATFVP(XP)-C Zeptometrix 18 x 0.5mL 645.92 EUR
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NATtrol GI Controls (12 x 0.2 mL)

NATGIC-BIO Zeptometrix 12 x 0.2 mL 441.04 EUR
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NATtrol MRSA Verification Panel (6 X 0.5mL)

NATMRSANP-C Zeptometrix 6 X 0.5mL 398.4 EUR
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NATtrol MRSA Panel (4 X 0.5 mL)

NATMRSAP-C Zeptometrix 4 X 0.5 mL 284 EUR
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NATtrol Norovirus Negative Control (6 x 0.125mL)

NATROTA-6MC Zeptometrix 6 x 0.125mL 213.28 EUR
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NATtrol RP Controls (12 x 0.25 mL)

NATRPC-NNS Zeptometrix 12 x 0.25 mL 454.56 EUR
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NATtrol Respiratory Panel 2 (RP2) Controls (Ea)

NATRPC2-BIO Zeptometrix Ea 458.72 EUR
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NATtrol RSV Positive Control (6 x 0.5mL)

NATRSV-6C Zeptometrix 6 x 0.5mL 232 EUR
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NATtrol Respiratory Verification Panel (20 x 0.2mL)

NATRVP-GMK Zeptometrix 20 x 0.2mL 1018.24 EUR
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NATtrol Respiratory Verification Panel (20 x 0.25mL)

NATRVP-QIA Zeptometrix 20 x 0.25mL 772.8 EUR
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NATtrol Shigella sonnei (Stool Matrix) (0.5 mL)

NATSSO-GP Zeptometrix 0.5 mL 93.68 EUR
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NATtrol Salmonella typhimurium (Stool Matrix) (0.5 mL)

NATSTY-GP Zeptometrix 0.5 mL 93.68 EUR
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NATtrol Vaginal Panel (24 x 0.5 mL)

NATVP-BD Zeptometrix 24 x 0.5 mL 1003.68 EUR
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NATtrol Zika Virus (PRVABC59) Stock (Qualitative) (1mL)

NATZIKV(PRV)-ST Zeptometrix 1mL 1106.64 EUR
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NATtrol Zika Virus Stock (Qualitative) (1 mL)

NATZIKV-ST Zeptometrix 1 mL 1106.64 EUR
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NATtrol Zika Virus Stock (Qualitative) (1 mL)

TEST Zeptometrix 1 mL 1106.64 EUR
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NATtrol RSV Positive Control (6 X 0.5 mL)

MDZ047 Zeptometrix 6 X 0.5 mL 232 EUR
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NATtrol Norovirus Negative Control (6 X 0.5 mL)

MDZ052 Zeptometrix 6 X 0.5 mL 213.28 EUR
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NATtrol GBS Positive Control (6 x 0.5 mL)

MDZ053 Zeptometrix 6 x 0.5 mL 315.2 EUR
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NATtrol GBS Negative Control (6 X 0.5 mL)

MDZ054 Zeptometrix 6 X 0.5 mL 315.2 EUR
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NATtrol EV Positive Control (6 X 0.2 mL)

MDZ055 Zeptometrix 6 X 0.2 mL 216.4 EUR
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NATtrol EV Negative Control (6 X 0.2 mL)

MDZ056 Zeptometrix 6 X 0.2 mL 216.4 EUR
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NATtrol MRSA Positive Control (6 X 0.5 mL)

MDZ059 Zeptometrix 6 X 0.5 mL 315.2 EUR
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NATtrol SA Positive Control (6 X 0.5 mL)

MDZ061 Zeptometrix 6 X 0.5 mL 315.2 EUR
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NATtrol Adenovirus Type 01 Stock (Qualitative) (1 mL)

NATADV1-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Adenovirus Type 03 Stock (Qualitative) (1 mL)

NATADV3-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Adenovirus Type 31 Stock (Qualitative) (1 mL)

NATADV31-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Adenovirus Type 04 Stock (Qualitative) (1 mL)

NATADV4-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Adenovirus Type 40 (Stool Matrix) (0.5 mL)

NATADV40-GP Zeptometrix 0.5 mL 93.68 EUR
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NATtrol Adenovirus Type 40 Stock (Qualitative) (1 mL)

NATADV40-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Adenovirus Type 41 (Stool Matrix) (0.5 mL)

NATADV41-GP Zeptometrix 0.5 mL 93.68 EUR
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NATtrol Adenovirus Type 41 Stock (Qualitative) (1 mL)

NATADV41-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Adenovirus Type 05 Stock (Qualitative) (1 mL)

NATADV5-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Adenovirus Type 07A Stock (Qualitative) (1 mL)

NATADV7A-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol AdV/hMPV/HRV Control (6 X 0.5mL)

NATAMR-ERC Zeptometrix 6 X 0.5mL 232 EUR
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NATtrol BC/GP Panel (10 X 0.75 mL)

NATBC/GP-NNS Zeptometrix 10 X 0.75 mL 626.16 EUR
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NATtrol BC/GN Panel (12 X 0.75 mL)

NATBCGN-NNS Zeptometrix 12 X 0.75 mL 726 EUR
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NATtrol CT/NG Panel (17 X 1.2 mL)

NATCT/NGP-C Zeptometrix 17 X 1.2 mL 873.68 EUR
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NATtrol Influenza/RSV Negative Control (6 x 0.5mL)

NATCXVA9-6C Zeptometrix 6 x 0.5mL 226.8 EUR
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NATtrol Influenza Negative Control (6 X 0.5 mL)

NATCXVA9-6MC Zeptometrix 6 X 0.5 mL 232 EUR
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NATtrol Coxsackievirus Type A9 Stock (Qualitative) (1 mL)

NATCXVA9-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Coxsackievirus Type B3 Stock (Qualitative) (1 mL)

NATCXVB3-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Coxsackievirus Type B4 Stock (Qualitative) (1 mL)

NATCXVB4-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Coxsackievirus Type B5 Stock (Qualitative) (1 mL)

NATCXVB5-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Echovirus Type 11 Stock (Qualitative) (1 mL)

NATECHO11-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Echovirus Type 6 Stock (Qualitative) (1 mL)

NATECHO6-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol EV Negative Control (6 X 0.2 mL)

NATEVNEG-6MC Zeptometrix 6 X 0.2 mL 216.4 EUR
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NATtrol EV Positive Control (6 X 0.2 mL)

NATEVPOS-6MC Zeptometrix 6 X 0.2 mL 216.4 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Influenza B Virus Stock (Qualitative) (1 mL)

NATFLUB-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Influenza/RSV Positive Control (6 x 0.25mL)

NATFLURSV-6L Zeptometrix 6 x 0.25mL 268.4 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Influenza/RSV Verification Panel (21 x 0.5mL)

NATFRVP-C Zeptometrix 21 x 0.5mL 765.52 EUR
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NATtrol Influenza Verification Panel (7 x 1.0 mL)

NATFVP-JAN Zeptometrix 7 x 1.0 mL 474.32 EUR
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NATtrol Flu Verification Panel (7 X 0.5 mL)

NATFVP-NNS Zeptometrix 7 X 0.5 mL 394.24 EUR
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NATtrol Human Metapneumovirus (HMPV) Stock (Qualitative) (1 mL)

NATHMPV-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol GBS Negative Control (6 X 0.5 mL)

NATLAC-6MC Zeptometrix 6 X 0.5 mL 315.2 EUR
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NATtrol Meningitis/Encephalitis Panel (14 x 0.4 mL)

NATMEP-BIO Zeptometrix 14 x 0.4 mL 636.56 EUR
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NATtrol Mouse Hepatitis Virus (MHV) Stock (1 mL)

NATMHV-ST Zeptometrix 1 mL 1170.08 EUR
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NATtrol MRSA Positive Control (6 X 0.5 mL)

NATMRSA-6MC Zeptometrix 6 X 0.5 mL 307.92 EUR
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NATtrol MRSA/SA Panel (5 X 0.5 mL)

NATMRSA/SAP-C Zeptometrix 5 X 0.5 mL 340.16 EUR
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NATtrol SA Positive Control (6 X 0.5 mL)

NATMSSA-6MC Zeptometrix 6 X 0.5 mL 307.92 EUR
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NATtrol Norovirus GI Positive Control (6 x 0.125mL)

NATNOVI-6MC Zeptometrix 6 x 0.125mL 213.28 EUR
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NATtrol Norovirus Group I (Recombinant) Stock (1 mL)

NATNOVI-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol Norovirus GII Positive Control (6 x 0.125mL)

NATNOVII-6MC Zeptometrix 6 x 0.125mL 213.28 EUR
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NATtrol Norovirus Group II (Recombinant) Stock (1 mL)

NATNOVII-ST Zeptometrix 1 mL 1137.84 EUR
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NATtrol GBS Positive Control (6 x 0.25 mL)

NATSAG-6L Zeptometrix 6 x 0.25 mL 268.4 EUR
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NATtrol GBS Positive Control (6 X 0.5 mL)

NATSAG-6MC Zeptometrix 6 X 0.5 mL 315.2 EUR
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NATtrol Strep A Verification Panel (24 x 0.1mL)

NATSAVP1-C Zeptometrix 24 x 0.1mL 632.4 EUR
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NATtrol Strep A Negative Control (6 x 0.5mL)

NATSDG-6MC Zeptometrix 6 x 0.5mL 226.8 EUR
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NATtrol Strep A Positive Control (6 x 0.5mL)

NATSPY-6MC Zeptometrix 6 x 0.5mL 226.8 EUR
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NATtrol T.vaginalis Verification Panel (17 x 0.7 mL)

NATTVGP-C Zeptometrix 17 x 0.7 mL 645.92 EUR
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NATtrol T.vaginalis Negative Control (6 x 1.2 mL)

NATTVNEG-6MC Zeptometrix 6 x 1.2 mL 330.8 EUR
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NATtrol T.vaginalis Positive Control (6 x 1.2 mL)

NATTVPOS-6MC Zeptometrix 6 x 1.2 mL 330.8 EUR
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NATtrol RP Multimarker Control Pack (6 X 0.75 mL)

MDZ001 Zeptometrix 6 X 0.75 mL 493.04 EUR
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NATtrol Clostridium difficile Positive Control (6 X 0.5 mL)

MDZ040 Zeptometrix 6 X 0.5 mL 323.52 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Clostridium difficile Negative Control (6 X 0.5 mL)

MDZ041 Zeptometrix 6 X 0.5 mL 323.52 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)

MDZ045 Zeptometrix 6 X 0.5 mL 226.8 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Norovirus GI Positive Control (6 X 0.5 mL)

MDZ050 Zeptometrix 6 X 0.5 mL 213.28 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Norovirus GII Positive Control (6 X 0.5 mL)

MDZ051 Zeptometrix 6 X 0.5 mL 213.28 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol MRSA/SA Negative Control (6 X 0.5 mL)

MDZ060 Zeptometrix 6 X 0.5 mL 315.2 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol BK Virus Linearity Panel (6 X 0.25 mL)

NATBK-LIN Zeptometrix 6 X 0.25 mL 529.44 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol C. difficile Positive Control (6 x 0.25 mL)

NATCDI-6L Zeptometrix 6 x 0.25 mL 268.4 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Clostridium difficile Positive Control (6 x 0.5 mL)

NATCDI-6MC Zeptometrix 6 x 0.5 mL 315.2 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

GenExpert Cepheid