Nucleic Acid control

Development and validation of a quantitative real time PCR assay for BK virus.

Several studies have shown that BK viral load in plasma and urine are reliable markers for the detection of BK virus associated nephropathy (BKVAN) in renal transplant patients. Zeptometrix developed a quantitative real time PCR assay based on TaqMan technology for the measurement of BK viral load in plasma and urine.

Considering the high similarity of the nucleotide sequence of the BK virus (BKV) with the JC virus (JCV), we designed this assay to specifically amplify BKV. We determined the viral DNA recovery rate on manual (QIAGEN’s QIAamp DNA Blood Mini Kit) and automated (BioMerieux’s NucliSENS EasyMAG) extraction methods. Cepheid GenExpert was used as control.

The comparison showed a higher viral DNA recovery rate on the automated extraction (61-76% in plasma and 52-65% in urine) as compared to the manual method (49-52% in plasma and 33-56% in urine). Quantitation of the viral load was performed using an external standard curve that was constructed with serial dilution of a plasmid containing the full length of the BKV genome. Commercially available quantitative BKV standards showed good correlation with the plasmid standard. The reproducibility of the assay was determined based on the Ct values of the amplified products as well as in BK copies per milliliter of sample. This assay is linear over a 7 log range (10 to 1 × 10(7) copies per reaction), no cross-reactivity was detected with the closest-related polyomavirus JCV, as well as other viruses that may be found in immunocompromised patients, and human genomic DNA. The limit of detection of the assay is 300 copies per milliliter in both plasma and urine and the limit of quantitation is 1000 copies per milliliter using the Zeptometrix NATtrol BK Virus Linearity Panel (Gentaur Poland).

This real time PCR assay provides a reliable and sensitive method for the quantitation of BKV in plasma and urine samples.

NATTROL CHIKUNGUNYA VIR STOCK

NATCHIKV-ST Zeptometrix 1.0mL 1365.41 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol GI Verification Panel (EA)

NATGIP-QIA Zeptometrix EA 1002 EUR

NATtrol Parainfluenza Control (6 X 0.5mL)

NATPIV-ERC Zeptometrix 6 X 0.5mL 314.59 EUR
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NATtrol Rotavirus (Stool Matrix) (0.5 mL)

NATROTA-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Rotavirus Stock (Qualitative) (1 mL)

NATROTA-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol BCID Panel (23 x 0.2mL)

NATBCP-BIO Zeptometrix 23 x 0.2mL 1138.27 EUR
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NATtrol Candida/TV Positive Control (ea)

NATCTVPOS-BD Zeptometrix ea 489.31 EUR
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NATtrol BCID2 Panel (35 x 0.2mL)

NATBCP2-BIO Zeptometrix 35 x 0.2mL 1108 EUR

NATtrol Cytomegalovirus Stock (Quantitative) (1 mL)

NATCMV-STQ Zeptometrix 1 mL 816 EUR

NATtrol Influenza B Stock (Quantitative) (1mL)

NATFLUB-STQ Zeptometrix 1 mL 779 EUR

NATtrol H. influenzae Stock (Quantitative) (1mL)

NATHIN-STQ Zeptometrix 1 mL 816 EUR

NATtrol K. pneumoniae Stock (Quantitative) (1mL)

NATKPN-STQ Zeptometrix 1 mL 816 EUR

NATtrol M. catarrhalis Stock (Quantitative) (1mL)

NATMCA-STQ Zeptometrix 1 mL 816 EUR

NATtrol S. pneumoniae Stock (Quantitative) (1mL)

NATSPN-STQ Zeptometrix 1 mL 816 EUR

NATtrol Escherichia coli (Stool Matrix) (0.5 mL)

NATECO(933)-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Escherichia coli (Stool Matrix) (0.5 mL)

NATECO(ETEC)-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Entamoeba histolytica (Stool Matrix) (0.5 mL)

NATEHI(DS4)-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol EV Panel (20 X 0.2 mL)

NATEVP-C Zeptometrix 20 X 0.2 mL 721.44 EUR
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NATtrol Influenza Verification Panel (18 x 0.5mL)

NATFVP(XP)-C Zeptometrix 18 x 0.5mL 775.1 EUR
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NATtrol GI Controls (12 x 0.2 mL)

NATGIC-BIO Zeptometrix 12 x 0.2 mL 529.25 EUR
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NATtrol MRSA Verification Panel (6 X 0.5mL)

NATMRSANP-C Zeptometrix 6 X 0.5mL 478.08 EUR
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NATtrol MRSA Panel (4 X 0.5 mL)

NATMRSAP-C Zeptometrix 4 X 0.5 mL 340.8 EUR
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NATtrol Norovirus Negative Control (6 x 0.125mL)

NATROTA-6MC Zeptometrix 6 x 0.125mL 255.94 EUR
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NATtrol RP Controls (12 x 0.25 mL)

NATRPC-NNS Zeptometrix 12 x 0.25 mL 545.47 EUR
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NATtrol Respiratory Panel 2 (RP2) Controls (Ea)

NATRPC2-BIO Zeptometrix Ea 550.46 EUR
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NATtrol RSV Positive Control (6 x 0.5mL)

NATRSV-6C Zeptometrix 6 x 0.5mL 278.4 EUR
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NATtrol Respiratory Verification Panel (20 x 0.2mL)

NATRVP-GMK Zeptometrix 20 x 0.2mL 1221.89 EUR
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NATtrol Respiratory Verification Panel (20 x 0.25mL)

NATRVP-QIA Zeptometrix 20 x 0.25mL 927.36 EUR
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NATtrol Shigella sonnei (Stool Matrix) (0.5 mL)

NATSSO-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Salmonella typhimurium (Stool Matrix) (0.5 mL)

NATSTY-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Vaginal Panel (24 x 0.5 mL)

NATVP-BD Zeptometrix 24 x 0.5 mL 1204.42 EUR
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NATtrol Zika Virus (PRVABC59) Stock (Qualitative) (1mL)

NATZIKV(PRV)-ST Zeptometrix 1mL 1327.97 EUR
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NATtrol Zika Virus Stock (Qualitative) (1 mL)

NATZIKV-ST Zeptometrix 1 mL 1327.97 EUR
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NATtrol BV Negative Control (6 X 0.15mL)

NATBVNEG-BD Zeptometrix 6 X 0.15mL 345.79 EUR
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NATtrol BV Positive Control (6 X 0.15mL)

NATBVPOS-BD Zeptometrix 6 X 0.15mL 489.31 EUR
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NATtrol Clostridium difficile (Stool Matrix) (0.5 mL)

NATCDI-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Campylobacter jejuni (Stool Matrix) (0.5 mL)

NATCJE-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Coronavirus 229E Stock (Qualitative) (1 mL)

NATCOV(229E)-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Coronavirus NL63 Stock (Qualitative) (1 mL)

NATCOV(NL63)-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Coronavirus OC43 Stock (Qualitative) (1 mL)

NATCOV(OC43)-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Zika Virus Stock (Qualitative) (1 mL)

TEST Zeptometrix 1 mL 1327.97 EUR
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NATtrol Influenza AH3 Stock (Quantitative) (1 mL)

NATFLUAH3-STQ Zeptometrix 1 mL 779 EUR

NATtrol MERS-CoV Stock (Qualitative) (1 mL)

NATMERS-ST Zeptometrix 1 mL 583 EUR

NATtrol MRSA Positive Control (6 x 0.35mL)

NATMRSA-6L Zeptometrix 6 x 0.35mL 264 EUR

NATtrol Coronavirus SARS Stock (Qualitative) (1 mL)

NATSARS-ST Zeptometrix 1 mL 608 EUR

NATtrol S. aureus (MRSA) Stock (Quantitative) (1mL)

NATSAU-STQ Zeptometrix 1 mL 816 EUR

NATtrol RSV Positive Control (6 X 0.5 mL)

MDZ047 Zeptometrix 6 X 0.5 mL 278.4 EUR
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NATtrol Norovirus Negative Control (6 X 0.5 mL)

MDZ052 Zeptometrix 6 X 0.5 mL 255.94 EUR
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NATtrol GBS Positive Control (6 x 0.5 mL)

MDZ053 Zeptometrix 6 x 0.5 mL 378.24 EUR
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NATtrol GBS Negative Control (6 X 0.5 mL)

MDZ054 Zeptometrix 6 X 0.5 mL 378.24 EUR
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NATtrol EV Positive Control (6 X 0.2 mL)

MDZ055 Zeptometrix 6 X 0.2 mL 259.68 EUR
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NATtrol EV Negative Control (6 X 0.2 mL)

MDZ056 Zeptometrix 6 X 0.2 mL 259.68 EUR
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NATtrol MRSA Positive Control (6 X 0.5 mL)

MDZ059 Zeptometrix 6 X 0.5 mL 378.24 EUR
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NATtrol SA Positive Control (6 X 0.5 mL)

MDZ061 Zeptometrix 6 X 0.5 mL 378.24 EUR
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NATtrol Echovirus Type 11 Stock (Qualitative) (1 mL)

NATECHO11-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Echovirus Type 6 Stock (Qualitative) (1 mL)

NATECHO6-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol EV Negative Control (6 X 0.2 mL)

NATEVNEG-6MC Zeptometrix 6 X 0.2 mL 259.68 EUR
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NATtrol EV Positive Control (6 X 0.2 mL)

NATEVPOS-6MC Zeptometrix 6 X 0.2 mL 259.68 EUR
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NATtrol Influenza B Virus Stock (Qualitative) (1 mL)

NATFLUB-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Influenza/RSV Positive Control (6 x 0.25mL)

NATFLURSV-6L Zeptometrix 6 x 0.25mL 322.08 EUR
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NATtrol Influenza/RSV Verification Panel (21 x 0.5mL)

NATFRVP-C Zeptometrix 21 x 0.5mL 918.62 EUR
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NATtrol Influenza Verification Panel (7 x 1.0 mL)

NATFVP-JAN Zeptometrix 7 x 1.0 mL 569.18 EUR
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NATtrol Flu Verification Panel (7 X 0.5 mL)

NATFVP-NNS Zeptometrix 7 X 0.5 mL 473.09 EUR
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NATtrol Human Metapneumovirus (HMPV) Stock (Qualitative) (1 mL)

NATHMPV-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol GBS Negative Control (6 X 0.5 mL)

NATLAC-6MC Zeptometrix 6 X 0.5 mL 378.24 EUR
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NATtrol Meningitis/Encephalitis Panel (14 x 0.4 mL)

NATMEP-BIO Zeptometrix 14 x 0.4 mL 763.87 EUR
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NATtrol Mouse Hepatitis Virus (MHV) Stock (1 mL)

NATMHV-ST Zeptometrix 1 mL 1404.1 EUR
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NATtrol MRSA Positive Control (6 X 0.5 mL)

NATMRSA-6MC Zeptometrix 6 X 0.5 mL 369.5 EUR
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NATtrol MRSA/SA Panel (5 X 0.5 mL)

NATMRSA/SAP-C Zeptometrix 5 X 0.5 mL 408.19 EUR
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NATtrol SA Positive Control (6 X 0.5 mL)

NATMSSA-6MC Zeptometrix 6 X 0.5 mL 369.5 EUR
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NATtrol Norovirus GI Positive Control (6 x 0.125mL)

NATNOVI-6MC Zeptometrix 6 x 0.125mL 255.94 EUR
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NATtrol Norovirus Group I (Recombinant) Stock (1 mL)

NATNOVI-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Norovirus GII Positive Control (6 x 0.125mL)

NATNOVII-6MC Zeptometrix 6 x 0.125mL 255.94 EUR
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NATtrol Norovirus Group II (Recombinant) Stock (1 mL)

NATNOVII-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol GBS Positive Control (6 x 0.25 mL)

NATSAG-6L Zeptometrix 6 x 0.25 mL 322.08 EUR
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NATtrol Strep A Verification Panel (24 x 0.1mL)

NATSAVP1-C Zeptometrix 24 x 0.1mL 758.88 EUR
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NATtrol Strep A Negative Control (6 x 0.5mL)

NATSDG-6MC Zeptometrix 6 x 0.5mL 272.16 EUR
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NATtrol Strep A Positive Control (6 x 0.5mL)

NATSPY-6MC Zeptometrix 6 x 0.5mL 272.16 EUR
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NATtrol T.vaginalis Verification Panel (17 x 0.7 mL)

NATTVGP-C Zeptometrix 17 x 0.7 mL 775.1 EUR
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NATtrol T.vaginalis Negative Control (6 x 1.2 mL)

NATTVNEG-6MC Zeptometrix 6 x 1.2 mL 396.96 EUR
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NATtrol T.vaginalis Positive Control (6 x 1.2 mL)

NATTVPOS-6MC Zeptometrix 6 x 1.2 mL 396.96 EUR
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NATtrol Adenovirus Type 01 Stock (Qualitative) (1 mL)

NATADV1-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Adenovirus Type 03 Stock (Qualitative) (1 mL)

NATADV3-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Adenovirus Type 31 Stock (Qualitative) (1 mL)

NATADV31-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Adenovirus Type 04 Stock (Qualitative) (1 mL)

NATADV4-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Adenovirus Type 40 (Stool Matrix) (0.5 mL)

NATADV40-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Adenovirus Type 40 Stock (Qualitative) (1 mL)

NATADV40-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Adenovirus Type 41 (Stool Matrix) (0.5 mL)

NATADV41-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Adenovirus Type 41 Stock (Qualitative) (1 mL)

NATADV41-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Adenovirus Type 05 Stock (Qualitative) (1 mL)

NATADV5-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Adenovirus Type 07A Stock (Qualitative) (1 mL)

NATADV7A-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol AdV/hMPV/HRV Control (6 X 0.5mL)

NATAMR-ERC Zeptometrix 6 X 0.5mL 278.4 EUR
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NATtrol BC/GP Panel (10 X 0.75 mL)

NATBC/GP-NNS Zeptometrix 10 X 0.75 mL 751.39 EUR
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NATtrol BC/GN Panel (12 X 0.75 mL)

NATBCGN-NNS Zeptometrix 12 X 0.75 mL 871.2 EUR
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NATtrol CT/NG Panel (17 X 1.2 mL)

NATCT/NGP-C Zeptometrix 17 X 1.2 mL 1048.42 EUR
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NATtrol Influenza/RSV Negative Control (6 x 0.5mL)

NATCXVA9-6C Zeptometrix 6 x 0.5mL 272.16 EUR
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NATtrol Influenza Negative Control (6 X 0.5 mL)

NATCXVA9-6MC Zeptometrix 6 X 0.5 mL 278.4 EUR
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NATtrol Coxsackievirus Type A9 Stock (Qualitative) (1 mL)

NATCXVA9-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Coxsackievirus Type B3 Stock (Qualitative) (1 mL)

NATCXVB3-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Coxsackievirus Type B4 Stock (Qualitative) (1 mL)

NATCXVB4-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Coxsackievirus Type B5 Stock (Qualitative) (1 mL)

NATCXVB5-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Adenovirus Type 1 Stock (Quantitative) (1 mL)

NATADV1-STQ Zeptometrix 1 mL 816 EUR

NATtrol Adenovirus Type 3 Stock (Quantitative) (1 mL)

NATADV3-STQ Zeptometrix 1 mL 816 EUR

NATtrol Epstein-Barr Virus Stock (Quantitative) (1 mL)

NATEBV-STQ Zeptometrix 1 mL 816 EUR

NATtrol Influenza A H1N1pdm Stock (Quantitative) (1 mL)

NATFLUAH1(2009)-STQ Zeptometrix 1 mL 816 EUR

NATtrol Influenza A H1 Stock (Quantitative) (1 mL)

NATFLUAH1-STQ Zeptometrix 1 mL 779 EUR

NATtrol GBS Negative Control (6 X 0.5 mL)

NATLAC-6MC-IVD Zeptometrix 6 X 0.5 mL 301 EUR

NATtrol MRSA Positive Control (6 X 0.5 mL)

NATMRSA-6MC-IVD Zeptometrix 6 X 0.5 mL 292 EUR

NATtrol SA Positive Control (6 X 0.5 mL)

NATMSSA-6MC-IVD Zeptometrix 6 X 0.5 mL 292 EUR

NATtrol Norovirus Negative Control (6 X 0.5 mL)

NATROTA-6MC-IVD Zeptometrix 6 X 0.5 mL 195 EUR

NATtrol RSV Positive Control (6 X 0.5 mL)

NATRSV-6C-IVD Zeptometrix 6 X 0.5 mL 216 EUR

NATtrol GBS Positive Control (6 x 0.5 mL)

NATSAG-6MC-IVD Zeptometrix 6 x 0.5 mL 301 EUR

NATtrol Varicella-Zoster Virus Stock (Quantitative) (1 mL)

NATVZV-STQ Zeptometrix 1 mL 816 EUR

NATtrol GBS Positive Control (6 X 0.5 mL)

NATSAG-6MC Zeptometrix 6 X 0.5 mL 378.24 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Influenza A H1N1pdm Stock | NATFLUAH1(2009)-STQ

2-NATFLUAH1(29)-STQ Zeptometrix 1 1327 EUR

NATtrol RP Multimarker Control Pack (6 X 0.75 mL)

MDZ001 Zeptometrix 6 X 0.75 mL 591.65 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Clostridium difficile Positive Control (6 X 0.5 mL)

MDZ040 Zeptometrix 6 X 0.5 mL 388.22 EUR
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NATtrol Clostridium difficile Negative Control (6 X 0.5 mL)

MDZ041 Zeptometrix 6 X 0.5 mL 388.22 EUR
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NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)

MDZ045 Zeptometrix 6 X 0.5 mL 272.16 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Norovirus GI Positive Control (6 X 0.5 mL)

MDZ050 Zeptometrix 6 X 0.5 mL 255.94 EUR
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NATtrol Norovirus GII Positive Control (6 X 0.5 mL)

MDZ051 Zeptometrix 6 X 0.5 mL 255.94 EUR
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NATtrol MRSA/SA Negative Control (6 X 0.5 mL)

MDZ060 Zeptometrix 6 X 0.5 mL 378.24 EUR
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NATtrol Influenza A/B Positive Control (6 x 0.5mL)

NATFLUAB-6C Zeptometrix 6 x 0.5mL 272.16 EUR
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NATtrol Influenza A H1 Virus Stock (Qualitative) (1 mL)

NATFLUAH1-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Influenza A H3 Virus Stock (Qualitative) (1 mL)

NATFLUAH3-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Influenza/RSV Positive Control (6 x 0.5 mL)

NATFLURSV-6C Zeptometrix 6 x 0.5 mL 272.16 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Human Rhinovirus Type 1A Stock(Qualitative) (1 mL)

NATHRV-STN Zeptometrix 1 mL 1365.41 EUR
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NATtrol Meningitis/Encephalitis (ME) Controls (12 x 0.2 mL)

NATMEC-BIO Zeptometrix 12 x 0.2 mL 490.56 EUR
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NATtrol Mycoplasma genitalium External Run Control (6 x 0.25mL)

NATMGN-ERC Zeptometrix 6 x 0.25mL 349.54 EUR
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NATtrol MRSA/SA Negative Control (6 X 0.5 mL)

NATMSSE-6MC Zeptometrix 6 X 0.5 mL 369.5 EUR
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NATtrol Neisseria gonorrhoeae Positive Control (6 X 1 mL)

NATNG-6MC Zeptometrix 6 X 1 mL 396.96 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Norovirus GI/GII Positive Control (6 x 0.125mL)

NATNOV-6MC Zeptometrix 6 x 0.125mL 255.94 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Norovirus Group I (Recombinant) (Stool Matrix) (0.5 mL)

NATNOVI-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Norovirus Group II (Recombinant) (Stool Matrix) (0.5 mL)

NATNOVII-GP Zeptometrix 0.5 mL 112.42 EUR
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NATtrol Parainfluenza Virus Type 1 Stock (Qualitative) (1 mL)

NATPARA1-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Parainfluenza Virus Type 2 Stock (Qualitative) (1 mL)

NATPARA2-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Parainfluenza Virus Type 3 Stock (Qualitative) (1 mL)

NATPARA3-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Parainfluenza Virus Type 4 Stock (Qualitative) (1 mL)

NATPARA4-ST Zeptometrix 1 mL 1365.41 EUR
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NATtrol Human Parvovirus B19 (10,000 IU/mL) (1 mL)

NATPARVO-0001 Zeptometrix 1 mL 318.34 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Human Parvovirus B19 (100,000 IU/mL) (1 mL)

NATPARVO-0002 Zeptometrix 1 mL 630.34 EUR
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NATtrol Human Parvovirus B19 (1,000,000 IU/mL) (1 mL)

NATPARVO-0003 Zeptometrix 1 mL 1200.67 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Human Parvovirus B19 (10,000,000 IU/mL) (1 mL)

NATPARVO-0004 Zeptometrix 1 mL 1362.91 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Human Parvovirus B19 (100,000,000 IU/mL) (1 mL)

NATPARVO-0005 Zeptometrix 1 mL 1689.89 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Respiratory Pathogen Panel -1 (6 x 0.25 mL)

NATRPP-1 Zeptometrix 6 x 0.25 mL 557.95 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Respiratory Verification Panel (RVP) (20 X 0.6 mL)

NATRVP-IDI Zeptometrix 20 X 0.6 mL 1020.96 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.

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