Development and validation of a quantitative real time PCR assay for BK virus.

Several studies have shown that BK viral load in plasma and urine are reliable markers for the detection of BK virus associated nephropathy (BKVAN) in renal transplant patients. Zeptometrix developed a quantitative real time PCR assay based on TaqMan technology for the measurement of BK viral load in plasma and urine.

Considering the high similarity of the nucleotide sequence of the BK virus (BKV) with the JC virus (JCV), we designed this assay to specifically amplify BKV. We determined the viral DNA recovery rate on manual (QIAGEN’s QIAamp DNA Blood Mini Kit) and automated (BioMerieux’s NucliSENS EasyMAG) extraction methods. Cepheid GenExpert was used as control.

The comparison showed a higher viral DNA recovery rate on the automated extraction (61-76% in plasma and 52-65% in urine) as compared to the manual method (49-52% in plasma and 33-56% in urine). Quantitation of the viral load was performed using an external standard curve that was constructed with serial dilution of a plasmid containing the full length of the BKV genome. Commercially available quantitative BKV standards showed good correlation with the plasmid standard. The reproducibility of the assay was determined based on the Ct values of the amplified products as well as in BK copies per milliliter of sample. This assay is linear over a 7 log range (10 to 1 × 10(7) copies per reaction), no cross-reactivity was detected with the closest-related polyomavirus JCV, as well as other viruses that may be found in immunocompromised patients, and human genomic DNA. The limit of detection of the assay is 300 copies per milliliter in both plasma and urine and the limit of quantitation is 1000 copies per milliliter using the Zeptometrix NATtrol BK Virus Linearity Panel (Gentaur Poland).

This real time PCR assay provides a reliable and sensitive method for the quantitation of BKV in plasma and urine samples.

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NATtrol S. pneumoniae Stock (Quantitative) (1mL)
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Description: Please contact Gentaur in order to receive the datasheet of the product.
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NATRSV-6C-IVD	Zeptometrix	6 X 0.5 mL	242 EUR
NATtrol GBS Positive Control (6 x 0.25 mL)
NATSAG-6L	Zeptometrix	6 x 0.25 mL	272 EUR
NATtrol GBS Positive Control (6 X 0.5 mL)
NATSAG-6MC	Zeptometrix	6 X 0.5 mL	313 EUR
NATtrol GBS Positive Control (6 x 0.5 mL)
NATSAG-6MC-IVD	Zeptometrix	6 x 0.5 mL	333 EUR
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Description: Please contact Gentaur in order to receive the datasheet of the product.
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Description: Please contact Gentaur in order to receive the datasheet of the product.
NATtrol GBS Negative Control (6 X 0.5 mL)
MDZ054	Zeptometrix	6 X 0.5 mL	378.24 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.
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NATMRSA-6MC-IVD	Zeptometrix	6 X 0.5 mL	335 EUR
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Description: Please contact Gentaur in order to receive the datasheet of the product.
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NATtrol S. aureus (MRSA) Stock (Quantitative) (1mL)
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NATCT/NGNEG-6MC	Zeptometrix	6 X 1 mL	220 EUR
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NATCJE-GP	Zeptometrix	0.5 mL	55 EUR
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NATtrol CT/NG/TV Positive Control (6 x 1.0mL)
NATCTNGTV-POS	Zeptometrix	6 x 1.0mL	368 EUR
NATtrol Human Parvovirus B19 (10,000 IU/mL) (1 mL)
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NATPARVO-0002	Zeptometrix	1 mL	592 EUR
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NATPARVO-0003	Zeptometrix	1 mL	1177 EUR
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NATADV40-GP	Zeptometrix	0.5 mL	55 EUR
NATtrol Adenovirus Type 41 (Stool Matrix) (0.5 mL)
NATADV41-GP	Zeptometrix	0.5 mL	55 EUR
NATtrol Mouse Hepatitis Virus (MHV) Stock (1 mL)
NATMHV-ST	Zeptometrix	1 mL	1319 EUR
NATtrol Strep A Verification Panel (24 x 0.1mL)
NATSAVP1-C	Zeptometrix	24 x 0.1mL	707 EUR
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NATtrol Coronavirus NL63 Stock (Qualitative) (1 mL)
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NATtrol Coronavirus OC43 Stock (Qualitative) (1 mL)
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NATEHI(DS4)-GP	Zeptometrix	0.5 mL	55 EUR
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NATtrol Coronavirus SARS Stock (Qualitative) (1 mL)
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NATtrol MRSA/SA Negative Control (6 X 0.5 mL)
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NATMSSE-6MC-IVD	Zeptometrix	6 X 0.5 mL	327 EUR
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Description: Please contact Gentaur in order to receive the datasheet of the product.
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NATCXVA9-6MC	Zeptometrix	6 X 0.5 mL	220 EUR
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Description: Please contact Gentaur in order to receive the datasheet of the product.
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Description: Please contact Gentaur in order to receive the datasheet of the product.
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Description: Please contact Gentaur in order to receive the datasheet of the product.
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MDZ001	Zeptometrix	6 X 0.75 mL	549 EUR
NATtrol Echovirus Type 11 Stock (Qualitative) (1 mL)
NATECHO11-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Echovirus Type 6 Stock (Qualitative) (1 mL)
NATECHO6-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Influenza/RSV Positive Control (6 x 0.25mL)
NATFLURSV-6L	Zeptometrix	6 x 0.25mL	272 EUR
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NATHSV-6L	Zeptometrix	6 x 0.25 mL	272 EUR
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NATtrol Adenovirus Type 01 Stock (Qualitative) (1 mL)
NATADV1-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Adenovirus Type 03 Stock (Qualitative) (1 mL)
NATADV3-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Adenovirus Type 31 Stock (Qualitative) (1 mL)
NATADV31-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Adenovirus Type 04 Stock (Qualitative) (1 mL)
NATADV4-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Adenovirus Type 40 Stock (Qualitative) (1 mL)
NATADV40-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Adenovirus Type 41 Stock (Qualitative) (1 mL)
NATADV41-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Adenovirus Type 05 Stock (Qualitative) (1 mL)
NATADV5-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Influenza B Virus Stock (Qualitative) (1 mL)
NATFLUB-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Influenza/RSV Verification Panel (21 x 0.5mL)
NATFRVP-C	Zeptometrix	21 x 0.5mL	862 EUR
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NATtrol Norovirus Group II (Recombinant) Stock (1 mL)
NATNOVII-ST	Zeptometrix	1 mL	1281 EUR
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NATCT/NGNEG-6MC-IVD	Zeptometrix	6 X 1.25 mL	240 EUR
NATtrol CT/NG Negative Control Pack (6 X 1.25 mL)
MDZ004	Zeptometrix	6 X 1.25 mL	299.62 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.
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NATCDI-6L	Zeptometrix	6 x 0.25 mL	272 EUR
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NATRPP-1	Zeptometrix	6 x 0.25 mL	514 EUR
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MDZ050	Zeptometrix	6 X 0.5 mL	205 EUR
NATtrol Adenovirus Type 1 Stock (Quantitative) (1 mL)
NATADV1-STQ	Zeptometrix	1 mL	857 EUR
NATtrol Adenovirus Type 3 Stock (Quantitative) (1 mL)
NATADV3-STQ	Zeptometrix	1 mL	857 EUR
NATtrol Adenovirus Type 07A Stock (Qualitative) (1 mL)
NATADV7A-ST	Zeptometrix	1 mL	1281 EUR
NATtrol SARS-CoV-2 (recombinant) Stock (1 x 1.0mL)
0831042	Zeptometrix	-	693 EUR
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NATCXVA9-6C-IVD	Zeptometrix	6 X 0.5 mL	240 EUR
NATtrol Influenza A/B Positive Control (6 x 0.5mL)
NATFLUAB-6C	Zeptometrix	6 x 0.5mL	211 EUR
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NATFLURSV-6C	Zeptometrix	6 x 0.5 mL	221 EUR
NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)
MDZ045	Zeptometrix	6 X 0.5 mL	272.16 EUR
Description: Please contact Gentaur in order to receive the datasheet of the product.
NATtrol Norovirus GII Positive Control (6 X 0.5 mL)
MDZ051	Zeptometrix	6 X 0.5 mL	205 EUR
NATtrol Epstein-Barr Virus Stock (Quantitative) (1 mL)
NATEBV-STQ	Zeptometrix	1 mL	857 EUR
NATtrol Influenza A H1N1pdm Stock (Quantitative) (1 mL)
NATFLUAH1(2009)-STQ	Zeptometrix	1 mL	857 EUR
NATtrol Respiratory Panel 2.1 (RP2.1) Controls (12 x 0.3mL)
NATRPC2.1-BIO	Zeptometrix	12 x 0.3mL	529 EUR
NATtrol MTB Verification Panel (5 x 0.6mL, 6 x 1.6mL)
NATMTBP-C	Zeptometrix	5 x 0.6mL, 6 x 1.6mL	613 EUR
NATtrol Coxsackievirus Type A9 Stock (Qualitative) (1 mL)
NATCXVA9-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Coxsackievirus Type B3 Stock (Qualitative) (1 mL)
NATCXVB3-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Coxsackievirus Type B4 Stock (Qualitative) (1 mL)
NATCXVB4-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Coxsackievirus Type B5 Stock (Qualitative) (1 mL)
NATCXVB5-ST	Zeptometrix	1 mL	1281 EUR
NATtrol Norovirus GI/GII Positive Control (6 x 0.125mL)
NATNOV-6MC	Zeptometrix	6 x 0.125mL	205 EUR

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