Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging
Glycolysis is the metabolic pathway that converts glucose into pyruvate, whereas fermentation can then produce lactate from pyruvate. Here, we developed single fluorescent protein (FP)-based lactate and pyruvate indicators with low EC50 for hint detection of metabolic molecules and live cell imaging and named them “Green Lindoblum” and “Green Pegassos,” respectively.
Green Lindoblum (EC50 of 30 µM for lactate) and Green Pegassos (EC50 of 70 µM for pyruvate) produced a 5.2- and 3.3-fold change in fluorescence depth in response to lactate and pyruvate, respectively. Green Lindoblum measured lactate ranges in mouse plasma, and Green Pegassos together with D-serine dehydratase efficiently estimated D-serine ranges launched from mouse main cultured neurons and astrocytes by measuring pyruvate degree.
Furthermore, live cell imaging evaluation revealed their utility for dual-colour imaging, and the interaction between lactate, pyruvate, and Ca2+ in human induced pluripotent stem cell-derived cardiomyocytes. Therefore, Green Lindoblum and Green Pegassos might be helpful instruments that detect particular molecules in scientific use and monitor the interaction of metabolites and different associated molecules in various cell varieties.
Promoter Pull-Down Assay: A Biochemical Screen for DNA-Binding Proteins
Transcription components are ubiquitous proteins that affiliate with promoter DNA and regulate gene expression by way of quite a lot of mechanisms. Understanding transcriptional management mechanisms requires in-depth investigation of the binding of transcription components to the promoters they regulate.
There are many in vivo and in vitro strategies for testing the binding of a identified protein to a promoter, reminiscent of chromatin immunoprecipitation and electrophoretic mobility shift assays. However, for these experiments, one will need to have a protein candidate to check and is just not capable of determine unknown proteins sure to a selected promoter.
Thus, the promoter pull-down assay was developed to fill this void. This technique makes use of DNA as bait to seize proteins that bind to a particular promoter, reminiscent of transcription components, from mobile lysates. Coupled with different experiments, the promoter pull-down assay vastly improves the repertoire of strategies obtainable for defining regulatory complexes that affect transcription.
Target-Based Identification and Optimization of 5-Indazol-5-yl Pyridones as Toll-like Receptor 7 and 8 Antagonists Using a Biochemical TLR8 Antagonist Competition Assay
Inappropriate activation of endosomal TLR7 and TLR8 happens in a number of autoimmune illnesses, particularly systemic lupus erythematosus (SLE). Herein, the event of a TLR8 antagonist competitors assay and its software for hit era of twin TLR7/Eight antagonists are reported.
The structure-guided optimization of the pyridone hit <b>3</b> utilizing this biochemical assay together with mobile and TLR8 cocrystal structural information resulted within the identification of a extremely potent and selective TLR7/Eight antagonist (<b>27</b>) with <i>in vivo</i> efficacy.
The two key steps for optimization have been (i) a core morph guided by a TLR7 sequence alignment to attain a twin TLR7/Eight antagonism profile and (ii) introduction of a fluorine within the piperidine ring to scale back its basicity, leading to enticing oral pharmacokinetic (PK) properties and improved TLR8 binding affinity.
Hunting monolignol transporters: membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce
Monolignol transport throughout lignification is {a partially} solved puzzle: each the mechanism(s) and the transported type of monolignols are unknown in creating xylem of timber. We examined a speculation of an energetic, plasma membrane (PM)-localized transport of monolignol monomers, dimers, and/or glucosidic types with membrane vesicles ready of creating xylem and lignin-forming tissue-cultured cells of Norway spruce (Picea abies L. Karst.), in addition to of management supplies, non-lignifying Norway spruce phloem and tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported each coniferin and p-coumaryl alcohol glucoside, however inhibitor assays prompt this transport being over the tonoplast.
Also membrane vesicles ready from the lignin-forming spruce cells confirmed coniferin transport, however the Km for coniferin was a lot increased than these of xylem and BY-2 cells. Liquid chromatography-mass spectrometry (LC-MS/MS) evaluation of membrane proteins remoted from spruce creating xylem, phloem and lignin-forming cultured cells revealed a number of transporters.
These have been in comparison with a transporter gene set that was gained by a correlation evaluation with a particular set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays confirmed no help for the ABC-transporter-mediated monolignol transport however level to secondary energetic transporters (reminiscent of MFS or MATE transporters). In distinction, proteomic and co-expression analyses counsel a job for ABC transporters and MFS transporters.
Protective Mechanism of Acacia saligna Butanol Extract and Its Nano-Formulations in opposition to Ulcerative Colitis in Rats as Revealed by way of Biochemical and Metabolomic Assays
Ulcerative colitis (UC) is a relapsing inflammatory illness of unknown etiology. The elevated danger of most cancers in UC sufferers warrants for the event of novel drug remedies. Herein, this work issues with the investigation of the protecting results of Acacia saligna butanol extract (ASBE) and its nanoformulations on UC in a rat mannequin and its underlying mechanism. Colitis was induced by gradual intrarectal infusion of two mL of 4% (v/v in 0.9% saline) acetic acid. Colon samples have been evaluated macroscopically, microscopically, and assayed for pro-inflammatory cytokine ranges.
To monitor related metabolic adjustments in acetic acid-induced UC mannequin, serum samples have been analyzed for main metabolites utilizing GC-MS adopted by multivariate information analyses. Treatment with ASBE attenuated acetic acid-induced UC as revealed by discount of colon weight, ulcer space, and ulcer index.
ASBE remedy additionally decreased Cyclooxygenase-2 (COX-2), Prostaglandin E2 (PGE2) & Interleukin-1β (IL-1β) ranges within the infected colon. The nano-formulation of ASBE confirmed higher safety than the crude extract in opposition to ulcer indices, elevated PGE2 manufacturing, and histopathological alterations reminiscent of intestinal mucosal lesions and inflammatory infiltration.
Distinct metabolite adjustments have been recorded in colitis rats together with a lower in oleamide and arachidonic acid together with elevated ranges of lactic acid, fructose, and pyroglutamic acid.
Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| DLR-bACE1-Mu-48T | DL Develop | 48T | 489 EUR |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) in samples from serum, plasma, tissue homogenates, cerebrospinal fluid or other biological fluids. |
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Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| DLR-bACE1-Mu-96T | DL Develop | 96T | 635 EUR |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) in samples from serum, plasma, tissue homogenates, cerebrospinal fluid or other biological fluids. |
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Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| DLR-bACE1-Ra-48T | DL Develop | 48T | 508 EUR |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) in samples from serum, plasma, cerebrospinal fluid or other biological fluids. |
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Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| DLR-bACE1-Ra-96T | DL Develop | 96T | 661 EUR |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) in samples from serum, plasma, cerebrospinal fluid or other biological fluids. |
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Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Hu-48Tests | Reddot Biotech | 48 Tests | 500 EUR |
Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Hu-96Tests | Reddot Biotech | 96 Tests | 692 EUR |
Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Mu-48Tests | Reddot Biotech | 48 Tests | 511 EUR |
Mouse Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Mu-96Tests | Reddot Biotech | 96 Tests | 709 EUR |
Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Ra-48Tests | Reddot Biotech | 48 Tests | 534 EUR |
Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit |
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| RDR-bACE1-Ra-96Tests | Reddot Biotech | 96 Tests | 742 EUR |
Bace1/ Rat Bace1 ELISA Kit |
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| ELI-02505r | Lifescience Market | 96 Tests | 886 EUR |
Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed |
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| ELISA-1 | Alpha Diagnostics | 1 | 202 EUR |
BACE1 |
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| RA21010 | Neuromics | 50 ug | 435 EUR |
Sheep BACE1 ELISA Kit |
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| ESB0322 | Abclonal | 96Tests | 521 EUR |
Mouse bACE1 ELISA Kit |
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| EMB0322 | Abclonal | 96Tests | 521 EUR |
Anserini bACE1 ELISA Kit |
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| EAB0322 | Abclonal | 96Tests | 521 EUR |
Rabbit BACE1 ELISA Kit |
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| ERTB0322 | Abclonal | 96Tests | 521 EUR |
Monkey BACE1 ELISA Kit |
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| EMKB0322 | Abclonal | 96Tests | 521 EUR |
Porcine BACE1 ELISA Kit |
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| EPB0322 | Abclonal | 96Tests | 521 EUR |
Rat BACE1 ELISA Kit |
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| ERB0322 | Abclonal | 96Tests | 521 EUR |
Human BACE1 ELISA Kit |
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| EHB0322 | Abclonal | 96Tests | 521 EUR |
Canine BACE1 ELISA Kit |
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| ECB0322 | Abclonal | 96Tests | 521 EUR |
Chicken BACE1 ELISA Kit |
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| ECKB0322 | Abclonal | 96Tests | 521 EUR |
Goat BACE1 ELISA Kit |
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| EGTB0322 | Abclonal | 96Tests | 521 EUR |
Bovine BACE1 ELISA Kit |
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| EBB0322 | Abclonal | 96Tests | 521 EUR |
Human BACE1 ELISA Kit |
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| ELA-E0718h | Lifescience Market | 96 Tests | 824 EUR |
BACE1 ELISA KIT|Human |
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| EF006629 | Lifescience Market | 96 Tests | 689 EUR |
BACE1 Antibody |
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| 1-CSB-PA002524GA01HU | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
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BACE1 Antibody |
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| 1-CSB-PA002524LA01HU | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
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BACE1 Antibody |
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| 1-CSB-PA008418 | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000 |
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BACE1 Antibody |
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| 1-CSB-PA150463 | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:5000, WB:1:500-1:2000 |
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BACE1 Antibody |
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| 1-CSB-PA173556 | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:15-1:50 |
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BACE1 protein |
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| E62B011 | EnoGene | 20ug | 343 EUR |
BACE1 Antibody |
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| 35650-100ul | SAB | 100ul | 252 EUR |
bACE1 siRNA |
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| 20-abx908826 | Abbexa |
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bACE1 siRNA |
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| 20-abx908827 | Abbexa |
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bACE1 siRNA |
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| 20-abx900582 | Abbexa |
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BACE1 Antibody |
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| ABD7939 | Lifescience Market | 100 ug | 438 EUR |
BACE1 Antibody |
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| ABD7999 | Lifescience Market | 100 ug | 438 EUR |
BACE1 Antibody |
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| DF7939 | Affbiotech | 200ul | 304 EUR |
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Description: BACE1 Antibody detects endogenous levels of total BACE1. |
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BACE1 antibody |
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| 70R-15953 | Fitzgerald | 50 ul | 435 EUR |
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Description: Rabbit polyclonal BACE1 antibody |
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BACE1 antibody |
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| 20R-BR014 | Fitzgerald | 50 ug | 656 EUR |
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Description: Rabbit polyclonal BACE1 antibody |
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BACE1 antibody |
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| 20R-BR015 | Fitzgerald | 50 ug | 656 EUR |
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Description: Rabbit polyclonal BACE1 antibody |
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BACE1 antibody |
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| 70R-7276 | Fitzgerald | 50 ug | 467 EUR |
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Description: Rabbit polyclonal BACE1 antibody raised against the N terminal of BACE1 |
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Bace1 antibody |
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| 70R-8662 | Fitzgerald | 50 ug | 467 EUR |
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Description: Affinity purified rabbit polyclonal Bace1 antibody |
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BACE1 Antibody |
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| 6651-100 | Biovision | 349 EUR | |
BACE1 Antibody |
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| 6651-30T | Biovision | 146 EUR | |
anti-BACE1 |
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| YF-PA17994 | Abfrontier | 100 ug | 403 EUR |
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Description: Rabbit polyclonal to BACE1 |
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BACE1 Antibody |
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| AF6650 | Affbiotech | 100ul | 350 EUR |
Guinea Pig BACE1 ELISA Kit |
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| EGB0322 | Abclonal | 96Tests | 521 EUR |
BACE1 ELISA Kit (Human) (OKCD06723) |
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| OKCD06723 | Aviva Systems Biology | 96 Wells | 753 EUR |
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Description: Description of target: Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein. BACE1, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi.Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Four transcript variants encoding different isoforms have been described for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.11ng/mL |
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BACE1 ELISA Kit (Mouse) (OKCD06724) |
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| OKCD06724 | Aviva Systems Biology | 96 Wells | 779 EUR |
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Description: Description of target: Bace1 is responsible for the proteolytic processing of the amyloid precursor protein (APP). Bace1 cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 12.1pg/mL |
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BACE1 ELISA Kit (Mouse) (OKEH03206) |
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| OKEH03206 | Aviva Systems Biology | 96 Wells | 662 EUR |
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Description: Description of target: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.39 ng/mL |
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BACE1 ELISA Kit (Human) (OKAN05558) |
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| OKAN05558 | Aviva Systems Biology | 96 Wells | 792 EUR |
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Description: Description of target: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.11 ng/mL |
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BACE1 ELISA Kit (Mouse) (OKAN05559) |
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| OKAN05559 | Aviva Systems Biology | 96 Wells | 792 EUR |
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Description: Description of target: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. Homozygous knockout mice for this gene exhibit a wide range of nervous system defects, growth retardation, metabolic abnormalities, and increased neonatal lethality.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 14.1 pg/mL |
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BACE1 ELISA Kit (Rat) (OKCD02313) |
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| OKCD02313 | Aviva Systems Biology | 96 Wells | 818 EUR |
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Description: Description of target: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. ;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 6.7 pg/mL |
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BACE1 cloning plasmid |
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| CSB-CL002524HU-10ug | Cusabio | 10ug | 531 EUR |
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Description: A cloning plasmid for the BACE1 gene. |
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BACE1-IN-1 |
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| HY-100182 | MedChemExpress | 5mg | 2465 EUR |
Anti-BACE1 Antibody |
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| STJ193186 | St John's Laboratory | 200 µl | 197 EUR |
Anti-BACE1 antibody |
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| STJ29911 | St John's Laboratory | 100 µl | 277 EUR |
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Description: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. |
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Polyclonal BACE1 Antibody |
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| APG03188G | Leading Biology | 0.1mg | 484 EUR |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BACE1 . This antibody is tested and proven to work in the following applications: |
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bACE1 (pS498) Antibody |
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| abx148520-100ug | Abbexa | 100 ug | 439 EUR |
BACE1 Rabbit pAb |
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| A5266-100ul | Abclonal | 100 ul | 308 EUR |
BACE1 Rabbit pAb |
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| A5266-200ul | Abclonal | 200 ul | 459 EUR |
BACE1 Rabbit pAb |
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| A5266-20ul | Abclonal | 20 ul | 183 EUR |
BACE1 Rabbit pAb |
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| A5266-50ul | Abclonal | 50 ul | 223 EUR |
anti- BACE1 antibody |
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| FNab00781 | FN Test | 100µg | 548.75 EUR |
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Description: Antibody raised against BACE1 |
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BACE1 Conjugated Antibody |
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| C35650 | SAB | 100ul | 397 EUR |
Recombinant Human BACE1 |
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| P0562 | FN Test | 100ug | 522.36 EUR |
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Description: Recombinant Human protein for BACE1 |
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BACE1 Polyclonal Antibody |
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| ABP-PAB-31005 | Allele Biotech | 50 ug | Ask for price |
BACE1 Polyclonal Antibody |
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| ABP-PAB-31006 | Allele Biotech | 50 ug | Ask for price |
BACE1 Blocking Peptide |
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| DF7939-BP | Affbiotech | 1mg | 195 EUR |
BACE1 Blocking Peptide |
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| 33R-3496 | Fitzgerald | 100 ug | 180 EUR |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of BACE1 antibody, catalog no. 70R-7276 |
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BACE1 Recombinant Protein |
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| 96-050 | ProSci | 0.1 mg | 516.5 EUR |
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Description: Beta-secretase 1 (BACE1) is also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. |
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BACE1 Recombinant Protein |
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| 96-051 | ProSci | 0.1 mg | 464 EUR |
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Description: Beta-secretase 1 (BACE1) is also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. |
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BACE1 Recombinant Protein |
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| 96-052 | ProSci | 0.2 mg | 464 EUR |
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Description: Beta-secretase 1 (BACE1) is also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. |
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Anti-BACE1 antibody |
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| PAab00781 | Lifescience Market | 100 ug | 386 EUR |
T&A Cloning Kits |
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| FYC001-20P | Yeastern Biotech | 1 vial | Ask for price |
Necrosis vs Apoptosis Kits |
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| KF17371 | Neuromics | 50-100 Tests | 435 EUR |
Necrosis vs Apoptosis Kits |
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| KF17372 | Neuromics | 100-200 Tests | 768 EUR |
Basic Cytotoxicity Test Kits |
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| CT17001 | Neuromics | 125 Tests | 409 EUR |
Basic Cytotoxicity Test Kits |
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| CT17002 | Neuromics | 250 Tests | 650 EUR |
Total Cytotoxicity Test Kits |
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| CT17003 | Neuromics | 125 Tests | 513 EUR |
Total Cytotoxicity Test Kits |
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| CT17004 | Neuromics | 250 Tests | 813 EUR |
Pipette Stand Starter Kits |
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| P7700-S1 | Benchmark Scientific | 1 PC | 507.83 EUR |
Pipette Stand Starter Kits |
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| P7700-S2 | Benchmark Scientific | 1 PC | 526.1 EUR |
BACE1 Antibody, HRP conjugated |
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| 1-CSB-PA002524LB01HU | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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BACE1 Antibody, FITC conjugated |
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| 1-CSB-PA002524LC01HU | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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BACE1 Antibody, Biotin conjugated |
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| 1-CSB-PA002524LD01HU | Cusabio |
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Description: A polyclonal antibody against BACE1. Recognizes BACE1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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Rat BACE1 shRNA Plasmid |
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| 20-abx985469 | Abbexa |
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Mouse BACE1 shRNA Plasmid |
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| 20-abx973511 | Abbexa |
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Human BACE1 shRNA Plasmid |
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| 20-abx958402 | Abbexa |
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BACE1 recombinant monoclonal antibody |
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| A5095 | Bimake | 100ul X 3 | 595 EUR |
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Description: A recombinant monoclonal antibody from rabbit against human BACE1 for WB,ELISA |
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BACE1 Recombinant Protein (Mouse) |
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| RP118622 | ABM | 100 ug | Ask for price |
BACE1 Recombinant Protein (Mouse) |
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| RP118625 | ABM | 100 ug | Ask for price |
BACE1 Recombinant Protein (Rat) |
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| RP191804 | ABM | 100 ug | Ask for price |
BACE1 Recombinant Protein (Human) |
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| RP002572 | ABM | 100 ug | Ask for price |
Phospho-BACE1 (Thr252) Antibody |
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| AF3650 | Affbiotech | 100ul | 450 EUR |
Rat Neural Stem Cell Kits |
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| PC37123 | Neuromics | 1 X 10(6) *minimum order 6 vials* | 957 EUR |
MitoPT™ TMRE & TMRM Kits |
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| KF17358 | Neuromics | 500 Test | 479 EUR |
MitoPT™ TMRE & TMRM Kits |
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| KF17359 | Neuromics | 500 Test | 479 EUR |
HISTAR DETECTION SYSTEM Kits (OKSA11259) |
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| OKSA11259 | Aviva Systems Biology | 50 Tests | 565 EUR |
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Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
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HISTAR DETECTION SYSTEM Kits (OKSA11260) |
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| OKSA11260 | Aviva Systems Biology | 150 Tests | 1335 EUR |
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Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
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HISTAR DETECTION SYSTEM Kits (OKSA11261) |
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| OKSA11261 | Aviva Systems Biology | 500 Tests | 2019 EUR |
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Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity: |
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Rat Bace1/ Beta-secretase 1 ELISA Kit |
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| E0115Ra | Sunlong | 1 Kit | 571 EUR |
Mouse Bace1/ Beta-secretase 1 ELISA Kit |
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| E0159Mo | Sunlong | 1 Kit | 571 EUR |
Human BACE1/ Beta-secretase 1 ELISA Kit |
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| E0249Hu | Sunlong | 1 Kit | 571 EUR |
BACE1 ELISA Kit (Human) : 96 Wells (OKEH00554) |
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| OKEH00554 | Aviva Systems Biology | 96 Wells | 662 EUR |
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Description: Description of target: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015];Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.31 ng/mL |
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Mouse Bace1(Beta-secretase 1) ELISA Kit |
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| EM0448 | FN Test | 96T | 524.1 EUR |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml |
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Mouse Beta- secretase 1, Bace1 ELISA KIT |
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| ELI-02502m | Lifescience Market | 96 Tests | 865 EUR |
Human Beta- secretase 1, BACE1 ELISA KIT |
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| ELI-02503h | Lifescience Market | 96 Tests | 824 EUR |
Bovine Beta- secretase 1, BACE1 ELISA KIT |
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| ELI-02504b | Lifescience Market | 96 Tests | 928 EUR |
Nori® Porcine 3-Plex ELISA Kits-Interferons |
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| GR224009 | Genorise Scientific | 96-well | 503 EUR |
Nori® Porcine 4-Plex ELISA Kits-Immunoglobulins |
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| GR224017 | Genorise Scientific | 96-well | 545 EUR |
Nori® Porcine 6-Plex ELISA Kits-BMP |
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| GR224025 | Genorise Scientific | 96-well | 629 EUR |
Nori® Porcine 6-Plex ELISA Kits-CCL |
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| GR224026 | Genorise Scientific | 96-well | 629 EUR |
Nori® Porcine 6-Plex ELISA Kits-MMP |
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| GR224027 | Genorise Scientific | 96-well | 629 EUR |
Nori® Porcine 6-Plex ELISA Kits-TLR |
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| GR224028 | Genorise Scientific | 96-well | 629 EUR |
Nori® Porcine 6-Plex ELISA Kits-Hormones |
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| GR224029 | Genorise Scientific | 96-well | 629 EUR |
Nori® Porcine 6-Plex ELISA Kits-SAA |
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| GR224030 | Genorise Scientific | 96-well | 629 EUR |
Nori® Porcine 6-Plex ELISA Kits-S100 |
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| GR224031 | Genorise Scientific | 96-well | 629 EUR |
Nori® Porcine 6-Plex ELISA Kits-CXCL |
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| GR224032 | Genorise Scientific | 96-well | 629 EUR |
Nori® Equine 3-Plex ELISA Kits-Interferons |
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| GR225009 | Genorise Scientific | 96-well | 503 EUR |
Nori® Equine 4-Plex ELISA Kits-Immunoglobulins |
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| GR225017 | Genorise Scientific | 96-well | 545 EUR |
Nori® Equine 6-Plex ELISA Kits-BMP |
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| GR225025 | Genorise Scientific | 96-well | 629 EUR |
Nori® Equine 6-Plex ELISA Kits-CCL |
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| GR225026 | Genorise Scientific | 96-well | 629 EUR |
Nori® Equine 6-Plex ELISA Kits-MMP |
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| GR225027 | Genorise Scientific | 96-well | 629 EUR |
Nori® Equine 6-Plex ELISA Kits-TLR |
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| GR225028 | Genorise Scientific | 96-well | 629 EUR |
Nori® Equine 6-Plex ELISA Kits-Hormones |
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| GR225029 | Genorise Scientific | 96-well | 629 EUR |
Nori® Equine 6-Plex ELISA Kits-SAA |
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| GR225030 | Genorise Scientific | 96-well | 629 EUR |
Nori® Equine 6-Plex ELISA Kits-S100 |
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| GR225031 | Genorise Scientific | 96-well | 629 EUR |
Nori® Equine 6-Plex ELISA Kits-CXCL |
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| GR225032 | Genorise Scientific | 96-well | 629 EUR |
Nori® Canine 3-Plex ELISA Kits-Interferons |
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| GR226009 | Genorise Scientific | 96-well | 503 EUR |
Nori® Canine 4-Plex ELISA Kits-Immunoglobulins |
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| GR226017 | Genorise Scientific | 96-well | 545 EUR |
Nori® Canine 6-Plex ELISA Kits-BMP |
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| GR226025 | Genorise Scientific | 96-well | 629 EUR |
Nori® Canine 6-Plex ELISA Kits-CCL |
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| GR226026 | Genorise Scientific | 96-well | 629 EUR |
Nori® Canine 6-Plex ELISA Kits-MMP |
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| GR226027 | Genorise Scientific | 96-well | 629 EUR |
Nori® Canine 6-Plex ELISA Kits-TLR |
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| GR226028 | Genorise Scientific | 96-well | 629 EUR |
Nori® Canine 6-Plex ELISA Kits-Hormones |
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| GR226029 | Genorise Scientific | 96-well | 629 EUR |
Nori® Canine 6-Plex ELISA Kits-SAA |
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| GR226030 | Genorise Scientific | 96-well | 629 EUR |
Nori® Canine 6-Plex ELISA Kits-S100 |
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| GR226031 | Genorise Scientific | 96-well | 629 EUR |
Nori® Canine 6-Plex ELISA Kits-CXCL |
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| GR226032 | Genorise Scientific | 96-well | 629 EUR |
Nori® Bovine 3-Plex ELISA Kits-Interferons |
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| GR228009 | Genorise Scientific | 96-well | 503 EUR |
Nori® Bovine 4-Plex ELISA Kits-Immunoglobulins |
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| GR228017 | Genorise Scientific | 96-well | 545 EUR |
Nori® Bovine 6-Plex ELISA Kits-BMP |
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| GR228025 | Genorise Scientific | 96-well | 629 EUR |
Nori® Bovine 6-Plex ELISA Kits-CCL |
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| GR228026 | Genorise Scientific | 96-well | 629 EUR |
Nori® Bovine 6-Plex ELISA Kits-MMP |
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| GR228027 | Genorise Scientific | 96-well | 629 EUR |
Nori® Bovine 6-Plex ELISA Kits-TLR |
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| GR228028 | Genorise Scientific | 96-well | 629 EUR |
Nori® Bovine 6-Plex ELISA Kits-Hormones |
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| GR228029 | Genorise Scientific | 96-well | 629 EUR |
Nori® Bovine 6-Plex ELISA Kits-SAA |
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| GR228030 | Genorise Scientific | 96-well | 629 EUR |
Nori® Bovine 6-Plex ELISA Kits-S100 |
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| GR228031 | Genorise Scientific | 96-well | 629 EUR |
Nori® Bovine 6-Plex ELISA Kits-CXCL |
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| GR228032 | Genorise Scientific | 96-well | 629 EUR |
Recombinant Human BACE1/ASP2 Protein |
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| RP00165 | Abclonal | 20 μg | 183 EUR |
Polyclonal BACE1 / BACE Antibody (Internal) |
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| APR11484G | Leading Biology | 0.05mg | 484 EUR |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BACE1 / BACE (Internal). This antibody is tested and proven to work in the following applications: |
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Polyclonal BACE1 Antibody (N-term) |
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| APG03026G | Leading Biology | 0.1ml | 484 EUR |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BACE1 (N-term). This antibody is tested and proven to work in the following applications: |
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Treatment with nano extract restored metabolite ranges to regular and means that cytokine ranges have been regulated by nano extract in UC. Conclusion: ASBE nano extract mitigated in opposition to acetic acid-induced colitis in rats, and the underlying mechanism might be attributed to the modulatory results of ASBE on the inflammatory cascades. The applicability of metabolomics developed on this rat mannequin appears to be essential for evaluating the anti-inflammatory mechanisms of latest therapeutics for acute colitis.
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