Description: Immortalized renal proximal tubule (RPT) cell line derived from primary rabbit kidney proximal tubule cell cultures which were transfected with the pRSV-T vector containing SV40 early region genes.The renal proximal tubule is part of the nephron (structural and functional unit of the kidney) containing the tubular fluid filtered through the glomerulus. The renal proximal tubule contains the components, proximal convulated tubule, loop of henle and the distal convulated tubule. Damage to the tubule can lead to kidney diseases such as Diabetes or Glomerular Disease.

Description: Immortalised Human Renal Proximal Tubule Cell Lines are Immortalised through infection with recombinant lentiviruses carrying SV40 Large T antigen

Description: The TH1 cells are derived from primary human renal proximal tubule epithelial cells (RPTECs) Immortalised by two lentiviral vectors carrying the human telomerase (hTERT) and the SV40 T antigen (Tag).Both the hTERT and Tag genes in the lentiviral vectors are flanked by loxP sites. Transient Cre expression in TH1 cells leads to efficient proviral deletion and upregulation of some renal specific activities. Removal of the genes for cell immortalization also significantly decreases the rate of cell proliferation and ultimately leads to cell senescence, suggesting that the cell cycle control remains intact in TH1 cells despite continuous passage of these cells in culture.

Description: Immortalised renal proximal tubule (RPT) cell line derived from primary rabbit kidney proximal tubule cell cultures which were transfected with the pRSV-T vector containing SV40 early region genes.The renal proximal tubule is part of the nephron (structural and functional unit of the kidney) containing the tubular fluid filtered through the glomerulus. The renal proximal tubule contains the components, proximal convulated tubule, loop of henle and the distal convulated tubule. Damage to the tubule can lead to kidney diseases such as Diabetes or Glomerular Disease.

Description: Renal proximal tubular epithelial cells (RPTEpiC) play a crucial role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. They are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases. RPTEpiC can produce inflammatory mediators such as cytokines or chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8. RPTEpiC express IL-2R alpha and MHC class II antigens during inflammation after renal transplantation or in crescentic glomerulonephritis, indicating the capacity to participate in the pathogenesis of immune renal injury. To be able to study the relationship between proximal tubular cells and a variety of renal diseases, the RPTEpiC culture provides a useful in vitro model.

Description: Rat Kidney Proximal Tubular Epithelial Cells from Gentaur are isolated from tissue of 5-day-old neonatal laboratory Sprague–Dawley Rats. Rat Kidney Proximal Tubular Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Kidney Proximal Tubular Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Kidney Proximal Tubular Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Description: Renal proximal tubular epithelial cells (PTEpiC) play a crucial role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. They are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases. PTEpiC can produce inflammatory mediators such as cytokines and chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8. PTEpiC express IL-2R alpha and MHC class II antigens during inflammation after renal transplantation or in crescentic glomerulonephritis indicating the capacity to participate in the pathogenesis of immune renal injury. To be able to study the relationship between tubular cells and a variety of renal diseases, the PTEpiC culture provides a useful in vitro model.HRPTEpiC from Gentaur Research Laboratories are isolated from human kidney. HRPTEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HRPTEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HRPTEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRPTEpiC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Description: C57BL/6 Mouse Primary Proximal Tubular Epithelial Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Proximal Tubular Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium for 3-7 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Proximal Tubular Epithelial Cells can be used in assays of cell to cell adhesion and migration. Standard biochemical procedures performed with epithelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.