When people talk about the end use of wood pulp, they usually don’t think of 3D cell culture. However, UPM has successfully developed GrowDex, an animal-free hydrogel extracted from birch wood, over the past decade. Birch is first processed into wood pulp along with pulp fibers, and then the wood pulp is used to make nanocellulose (NFC) hydrogels.

Since the beginning of the millennium, nanotechnology and its benefits have attracted widespread attention. Today, this technology has produced artificial meat, 3D printed organs and cosmetics that do not require animal testing. Although UPM started the research and development of nanocellulose and its applications very early, the real potential of nanocellulose did not appear until a long time later.

Innovative new materials

According to EU directives, animal products should be stopped as much as possible in the development of new drugs and cosmetics tests. Pekka Hurskainen, vice president of strategy and new business development at UPM, said, “We believe that this kind of hydrogel made of wood material has market demand.” The products of our competitors are generally developed through artificial synthesis or based on animals. , But our products are different. ”Hurskainen explained that“ our wood comes from sustainable and responsible forests, which is one of the reasons why our customers like us. Our raw materials are also certified by FSC® and PEFCTM. “

Innovation history

2008: UPM, together with other partners VTT and Aalto University, established the Finnish Nanocellulose Research Center in Otaniemi, Finland. In the same year, UPM collaborated with the University of Helsinki on the first cell culture experiment-as the beginning of GrowDex biomedical applications.

2012: The first scientific article related to GrowDex is released, and related research is in progress.

2014: GrowDex hydrogel products are launched.

3D cell culture case

Cell culture refers to the process of growing cells outside the natural growth environment and under controlled laboratory conditions, using 2D and 3D technologies. “3D is becoming more and more popular because it can simulate the natural environment of cells more than 2D culture. And it is generally believed that 3D culture is more biologically meaningful.

This is a new type of cell culture method. Hurskainen explained: “We evaluated the business case and the market potential of different applications and found that 3D cell culture is the most promising. “This is the key to UPM’s decision to focus on the application of GrowDex in the biopharmaceutical market.

Hurskainen added: “The cell culture market is a huge market worth about 12 billion euros, and is still growing rapidly. At the same time, the 3D cell culture field is currently worth 3 million euros, maintaining a steady growth of 20-30%.”

Biomedical applications

GrowDex hydrogels are biocompatible and provide an ideal environment to support cell growth and conduct cell research. Cells, spheroids (small tumors), and biopsy samples can all be cultured in GrowDex and used for research, such as drug response for new drug development or personalized drugs for cancer treatment.

Hydrogels can also be used for 3D printing, providing a way to create tissues or structures by combining with cells. Using human cells to create skin models for testing drugs, chemicals or cosmetics can replace animal experiments and provide more applicable data. In addition, the combination of GrowDex and cells into a scaffold can be used in tissue engineering in the future to manufacture human implants for tissue repair and regeneration.

3D is the future trend

3D cell culture has a wide range of applications, including in the field of veterinary medicine. UPM has identified the huge potential of hydrogels in medical innovation and is currently focusing on research in human applications.

“Pharmaceutical companies, biotechnology companies, research institutions, and hospitals all use cell culture. It is mainly used for cell research, development of different disease models, safety testing, drug development, regenerative medicine, and tissue engineering.” Hurskainen said. “In the existing application fields, the most important ones are cancer research, drug screening, stem cell and regenerative medicine,” he added. “In the long run, natural clinical applications such as wound healing, implants, and diagnosis are all Potential applications for hydrogels. “

Rt-Pcr kits

RTq-A569-100D Leuconostoc RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Leuconostoc .
RTq-A569-150D Leuconostoc RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Leuconostoc .
RTq-A569-50D Leuconostoc RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Leuconostoc .
RTq-A571-100D Pediococcus RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Pediococcus .
RTq-A571-150D Pediococcus RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Pediococcus .
RTq-A571-50D Pediococcus RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Pediococcus .
RTq-A635-100D Halobacillus RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Halobacillus .
RTq-A635-150D Halobacillus RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Halobacillus .
RTq-A635-50D Halobacillus RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Halobacillus .
RTq-H433-100R Enterovirus RT PCR kit | Bioingentech | 100T: 776.40 EUR Description: A Real-Time PCR kit for detection of Enterovirus .
RTq-H433-150R Enterovirus RT PCR kit | Bioingentech | 150T: 877.90 EUR Description: A Real-Time PCR kit for detection of Enterovirus .
RTq-H433-50R Enterovirus RT PCR kit | Bioingentech | 50T: 631.40 EUR Description: A Real-Time PCR kit for detection of Enterovirus .
RTq-H453-100R Mengovirus RT PCR kit | Bioingentech | 100T: 776.40 EUR Description: A Real-Time PCR kit for detection of Mengovirus .
RTq-H453-150R Mengovirus RT PCR kit | Bioingentech | 150T: 877.90 EUR Description: A Real-Time PCR kit for detection of Mengovirus .
RTq-H453-50R Mengovirus RT PCR kit | Bioingentech | 50T: 631.40 EUR Description: A Real-Time PCR kit for detection of Mengovirus .
RTq-H491-100R Astrovirus RT PCR kit | Bioingentech | 100T: 776.40 EUR Description: A Real-Time PCR kit for detection of Astrovirus .
RTq-H491-150R Astrovirus RT PCR kit | Bioingentech | 150T: 877.90 EUR Description: A Real-Time PCR kit for detection of Astrovirus .
RTq-H491-50R Astrovirus RT PCR kit | Bioingentech | 50T: 631.40 EUR Description: A Real-Time PCR kit for detection of Astrovirus .
RTq-H493-100R Calicivirus RT PCR kit | Bioingentech | 100T: 776.40 EUR Description: A Real-Time PCR kit for detection of Calicivirus .
RTq-H493-150R Calicivirus RT PCR kit | Bioingentech | 150T: 877.90 EUR Description: A Real-Time PCR kit for detection of Calicivirus .
RTq-H493-50R Calicivirus RT PCR kit | Bioingentech | 50T: 631.40 EUR Description: A Real-Time PCR kit for detection of Calicivirus .
RTq-H501-100D Adenovirus RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Adenovirus .
RTq-H501-150D Adenovirus RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Adenovirus .
RTq-H501-50D Adenovirus RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Adenovirus .
RTq-H515-100D Polyomavirus RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Polyomavirus .
RTq-H515-150D Polyomavirus RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Polyomavirus .
RTq-H515-50D Polyomavirus RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Polyomavirus .
RTq-H630-100R Sapovirus RT PCR kit | Bioingentech | 100T: 776.40 EUR Description: A Real-Time PCR kit for detection of Sapovirus .
RTq-H630-150R Sapovirus RT PCR kit | Bioingentech | 150T: 877.90 EUR Description: A Real-Time PCR kit for detection of Sapovirus .
RTq-H630-50R Sapovirus RT PCR kit | Bioingentech | 50T: 631.40 EUR Description: A Real-Time PCR kit for detection of Sapovirus .
RTq-H642-100R Parechovirus RT PCR kit | Bioingentech | 100T: 776.40 EUR Description: A Real-Time PCR kit for detection of Parechovirus .
RTq-H642-150R Parechovirus RT PCR kit | Bioingentech | 150T: 877.90 EUR Description: A Real-Time PCR kit for detection of Parechovirus .
RTq-H642-50R Parechovirus RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Parechovirus .
RTq-H643-100R Metapneumovirus RT PCR kit | Bioingentech | 100T: 776.40 EUR Description: A Real-Time PCR kit for detection of Metapneumovirus .
RTq-H643-150R Metapneumovirus RT PCR kit | Bioingentech | 150T: 877.90 EUR Description: A Real-Time PCR kit for detection of Metapneumovirus .
RTq-H643-50R Metapneumovirus RT PCR kit | Bioingentech | 50T: 631.40 EUR Description: A Real-Time PCR kit for detection of Metapneumovirus .
RTq-H683-100D Entamoeba RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Entamoeba .
RTq-H683-150D Entamoeba RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Entamoeba .
RTq-H683-50D Entamoeba RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Entamoeba .
RTq-H684-100D Acanthamoeba RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Acanthamoeba .
RTq-H684-150D Acanthamoeba RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Acanthamoeba .
RTq-H684-50D Acanthamoeba RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Acanthamoeba .
RTq-H722-100D Echovirus RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Echovirus .
RTq-H722-150D Echovirus RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Echovirus .
RTq-H722-50R Echovirus RT PCR kit | Bioingentech | 50T: 631.40 EUR Description: A Real-Time PCR kit for detection of Echovirus .
9K-002-0036 Wise RT/PCR Premix (96Rx) | BBI Biotech | 96Rx: 581.14 EUR
9K-005-0011 One Step RT PCR Kit | BBI Biotech | 100Rxn, 100prep: 267.50 EUR
G174 One-Step RT-PCR Kit | ABM | 100 x 50 ul reactions: 233.00 EUR
G281 OneScriptTwo-Step RT-PCR Kit | ABM | 25 reactions: 101.00 EUR
G282 OneScriptTwo-Step RT-PCR Kit | ABM | 100 reactions: 177.00 EUR
G928 RT-PCR Control Template Kit | ABM | 100 rxns: 205.00 EUR
RTq-V631-100D Angiostrongylus cantonensis RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Angiostrongylus cantonensis .
RTq-V631-150D Angiostrongylus cantonensis RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Angiostrongylus cantonensis .
RTq-V631-50D Angiostrongylus cantonensis RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Angiostrongylus cantonensis .
RTq-V634-100R Visna virus RT PCR kit | Bioingentech | 100T: 776.40 EUR Description: A Real-Time PCR kit for detection of Visna virus .
RTq-V634-150R Visna virus RT PCR kit | Bioingentech | 150T: 877.90 EUR Description: A Real-Time PCR kit for detection of Visna virus .
RTq-V634-50R Visna virus RT PCR kit | Bioingentech | 50T: 631.40 EUR Description: A Real-Time PCR kit for detection of Visna virus .
RTq-V726-100D Porcine adenovirus RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Porcine adenovirus .
RTq-V726-150D Porcine adenovirus RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Porcine adenovirus .
RTq-V726-50D Porcine adenovirus RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Porcine adenovirus .
RTq-V727-100D Swinepox virus RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Swinepox virus .
RTq-V727-150D Swinepox virus RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Swinepox virus .
RTq-V727-50D Swinepox virus RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Swinepox virus .
RTq-V729-100D Porcine enterovirus RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Porcine enterovirus .
RTq-V729-150D Porcine enterovirus RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Porcine enterovirus .
RTq-V729-50R Porcine enterovirus RT PCR kit | Bioingentech | 50T: 631.40 EUR Description: A Real-Time PCR kit for detection of Porcine enterovirus .
RTq-V730-100D Bovine parvovirus RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Bovine parvovirus .
RTq-V730-150D Bovine parvovirus RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Bovine parvovirus .
RTq-V730-50D Bovine parvovirus RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Bovine parvovirus .
RTq-VA030-100D Candida albicans RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Candida albicans .
RTq-VA030-150D Candida albicans RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Candida albicans .
RTq-VA030-50D Candida albicans RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Candida albicans .
RTq-VA206-100D Streptococcus uberis RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Streptococcus uberis .
RTq-VA206-150D Streptococcus uberis RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Streptococcus uberis .
RTq-VA206-50D Streptococcus uberis RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Streptococcus uberis .
RTq-VA208-100D Streptococcus agalactiae RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Streptococcus agalactiae .
RTq-VA208-150D Streptococcus agalactiae RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Streptococcus agalactiae .
RTq-VA208-50D Streptococcus agalactiae RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Streptococcus agalactiae .
RTq-VH002-100D Aspergillus fumigatus RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Aspergillus fumigatus .
RTq-VH002-150D Aspergillus fumigatus RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Aspergillus fumigatus .
RTq-VH002-50D Aspergillus fumigatus RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Aspergillus fumigatus .
RTq-VH005-100D Clostridium difficile RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Clostridium difficile .
RTq-VH005-150D Clostridium difficile RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Clostridium difficile .
RTq-VH005-50D Clostridium difficile RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Clostridium difficile .
RTq-VH007-100D Fusarium sp. RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Fusarium sp. .
RTq-VH007-150D Fusarium sp. RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Fusarium sp. .
RTq-VH007-50D Fusarium sp. RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Fusarium sp. .
RTq-VH013-100D Plasmodium sp. RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Plasmodium sp. .
RTq-VH013-150D Plasmodium sp. RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Plasmodium sp. .
RTq-VH013-50D Plasmodium sp. RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Plasmodium sp. .
RTq-VH019-100D Pasteurella multocida RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Pasteurella multocida .
RTq-VH019-150D Pasteurella multocida RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Pasteurella multocida .
RTq-VH019-50D Pasteurella multocida RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Pasteurella multocida .
RTq-VH024-100D Borrelia burgdorferi RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Borrelia burgdorferi .
RTq-VH024-150D Borrelia burgdorferi RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Borrelia burgdorferi .
RTq-VH024-50D Borrelia burgdorferi RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Borrelia burgdorferi .
RTq-VH025-100D Salmonella sp. RT PCR kit | Bioingentech | 100T: 628.50 EUR Description: A Real-Time PCR kit for detection of Salmonella sp. .
RTq-VH025-150D Salmonella sp. RT PCR kit | Bioingentech | 150T: 701.00 EUR Description: A Real-Time PCR kit for detection of Salmonella sp. .
RTq-VH025-50D Salmonella sp. RT PCR kit | Bioingentech | 50T: 532.80 EUR Description: A Real-Time PCR kit for detection of Salmonella sp. .

Tissue Cell Culture Related Products

Lifchem is the leading next day supplier of normal human cell culture products:

1. • Water Baths − Lab Companion
2. • Serological Pipettes
3. • FBS
4. • Syringe Filters
5. • Gloves
6. • Bunsen Burners
7. • Centrifuge Tubes
8. • Centrifuges
9. • Chairs
10. • Cryogenics
11. • Freezers
12. • Gloves
13. • Hoods
14. • Water−Jacketed CO2 Incubators
15. • Inverted Microscopes
16. • Pipette Controllers
17. • Refrigerators
18. • Serum
19. • Shakers
20. • Sterilizers
21. • AMRESCO Reagents
22. • Single Channel Pipettors
23. • Multi Channel Pipettors
24. • Electronic Pipettors
25. • Culture Hybridomas for Antibody Purification
26. • Cell Counting Kits

Prepare to subculture the cells when the culture has reached around 80% confluence.

The following protocol is for adherent cells; for suspension cells, start at step 6.

Protocol

1. Pre-warm the complete growth media given by the mobile line datasheet at a 37oC
   water bath. Pre-warm the Trypsin-EDTA (TM050) to room temperature.
2. Carefully aspirate the culture media in the culture vessel without bothering the
   cell monolayer.
3. Insert pre-warmed Trypsin-EDTA to the culture vessel. Gently rock the culture vessel
   To ensure complete coverage of this Trypsin-EDTA over the cells. (Table 1 provides
   Guidelines to the volumes of Trypsin-EDTA needed for a range of culture vessels).
4. Celebrate the cells under a microscope to confirm They’re dissociating from each
   other and are rounding up. Gently tap the culture vessel from several sides to
   promote cell detachment. Cells that are difficult to detach can be put in 37oC for
   Several moments to facilitate dispersal.
5. When majority of the cells have detached, add an equal quantity of the complete
   Media into the culture vessel to neutralize the trypsin-EDTA. Gently swirl or pipette
   The culture suspension to ensure the neutralization is complete.
6. Transfer the culture suspension into a sterile centrifuge tube.
7. Centrifuge the cell suspension at 1500 rpm for 3 minutes. The actual centrifuge
   Duration and speed may vary depending upon the cell type.
8. Aspirate the supernatant after checking all cells are pulled down into the pellet.
   Re-suspend the cell pellet in pre-warmed fresh complete media (adjust volume as
   necessary according to Table 1).
9. Pre-warm new culture vessels to 37 °C*. Seed cells in the recommended seeding density.
10. Set the newly seeded culture vessel at a 37oC*, 5% CO2 incubator. Incubate for at least
    24 – 48 hours before processing the cells for downstream experiments.
11. Renew the culture media every 2-3 days if the cells have not attained 80% confluency.
    Table 1: Volume of Trypsin-EDTA and Culture Media for Different Culture Vessels
    Culture Wares Trypsin-EDTA Volume Complete Media Volume
    12- well plate 0.5 ml/ well 2.0 ml/ well
    6- well plate 1.0 ml/ well 3.0 ml/ well
    T-25 flask 3.0 ml/ flask 7.0 ml/ flask
    T-75 flask 5.0 ml/ flask 20.0 ml/ flask
    100 mm dish 3.0 ml/ dish 10.0 ml/ dish
    150 mm dish 5.0 ml/ dish 20.0 ml/ dish

Welcome

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Azelastine Hydrochloride

Chemical Name:
(＼)-1-(2H)-phthalazinone,4-[(4-chlorophenyl) methyl]-2-(hexahydro-1-methyl-1H-azepin- 4-yl)-, monohydrochloride
CAS Number: [79307-93-0]
Molecular formula: C22H24ClN3O.HCl
Molecular Weight: 418.37
Quality Standard: Enterprise Standard

Hefei Lifang Fine Chemicals Co., Ltd is mainly producing drug midbody and fine chemicals, is located HighTea.Ind.DevelopZone Hefei Anhui.P.R.China.
Our company belongs to high-tech area. A high-tech team make our company capable of developing new chemicals and pharmacy technics. One of the advantages of our company is the self-contained equipments for medicine synthesis and special reactions, such as fluorating, hydriding, actinic, high temperature and press. These will further our development.
We pursue the finest technology, as we push to become a scale fine chemical research, manufacturing and trading enterprise in china.
We offer quality and serviles, uphold a good management system and company culture. we welcome your business.

Taq Polymerase 500 units / 44 USD